Paper chip and its preparation method and biomolecular detection method

Abstract

The invention discloses a paper chip, a biological molecule detection method adopting the paper chip, and a preparation method of the paper chip. The paper chip sequentially comprises an electrode layer, a flow distribution layer, and a sample feeding filtering layer, wherein the electrode layer is provided with a counter electrode, a reference electrode and a plurality of working electrodes, anda detection material is fixedly arranged at the surface of each working electrode; the flow distribution layer is overlapped on the electrode layer, and a reference electrode flow guide hole and a plurality of working electrode flow guide holes are formed in the flow distribution layer in a penetrating way, and are connected by a first flow guide channel; the sample feeding filtering layer is overlapped on the flow distribution layer, and is provided with a center flow distribution area and a plurality of flow distribution and guide holes, the center flow distribution area is positioned at theupper surface of the sample feeding filtering layer, the plurality of flow distribution and guide holes penetrate through the sample feeding filtering layer, the center flow distribution area and theplurality of flow distribution and guide holes are connected by a second flow guide channel, and a to-be-detected fluid flows into the center flow distribution area via a sample feeding channel; theto-be-detected fluid is guided into the working electrodes via the flow distribution and guide holes and the working electrode flow guide holes, and is guided into the counter electrode and the reference electrode via the reference electrode flow guide hole.

Description

technical field [0001] The embodiments of the present invention relate to the field of biological detection, in particular to a paper chip and a method for detecting biomolecules using the paper chip. Background technique [0002] The detection of biomolecules such as disease markers is an important means to understand the function of human organs and further evaluate human health. ) and other clinical indicators to evaluate ovarian reserve function. However, sex hormones such as FSH, LH, and E2 are extremely low in human blood, requiring high detection sensitivity. [0003] For the detection of FSH, LH and E2, there are currently mainly methods such as immunocolloidal gold test strips, immunobiochemical detectors, and microfluidic paper chips. Among them, the immunocolloidal gold test strip method is easy to operate and simple in structure, but it is qualitative or semi-quantitative detection, and the sensitivity is not high; the immunological biochemical detector can rea...

AI Technical Summary

Problems solved by technology

[0004] At present, most of the paper chips using the direct electrochemical detection method are drop-dropped samples, and the sample volume is large. In the process of developing the paper chip, different parameters will interfere with each other, and low-level sex hormones are easily interfered by electrode preparation modification conditions and environmental factors. Problems to be optimized in channel and micro-reaction area

Images