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Extraction method and application of adipose mesenchymal active cells of autologous germinal layer

An extraction method and germinal layer technology, applied in the field of medical cosmetology, can solve the problems of lack of adipose-derived mesenchymal active cells for beautification and weak proliferative ability of adipose-derived mesenchymal active cells, so as to reduce the formation of melanin, enhance activity, reduce side effects

Pending Publication Date: 2018-11-02
白晋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The object of the present invention is to provide a preparation method and application of adipose-derived mesenchymal active cell preparation, which is used to solve the problem of weak proliferative ability of adipose-derived mesenchymal active cells prepared by existing preparation methods and the lack of suitable adipose-derived mesenchymal active cells skin care

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  • Extraction method and application of adipose mesenchymal active cells of autologous germinal layer

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Embodiment 1

[0035] Example 1 Extraction method of autologous germinal layer adipose mesenchymal active cells of the present invention

[0036] The autologous germinal layer adipose mesenchymal active cell extraction method of the present invention comprises the following steps:

[0037] Step A: collecting adipose tissue, crushing it mechanically, and washing it with normal saline;

[0038] Step B: adding the broken adipose tissue to the buffer and stirring until clarified, removing damaged tissue, blood and red blood cells;

[0039] Step C: add the mixed enzyme solution of type I collagenase and type II collagenase with a mass concentration of 0.20% to the adipose tissue, mix evenly, culture on a shaking table, and centrifuge to obtain precipitated adipose mesenchymal active cells; wherein, the rotation speed of the shaking table culture is 100-200r / min, the time is 30-40min. The mass ratio of type I collagenase to type II collagenase is 1:1.

[0040] Step D: expand the precipitated ad...

Embodiment 2

[0046] Example 2: The adipose mesenchymal active cell preparation of the present invention is applied in plastic surgery.

[0047] Using DMEM medium containing 12% FBS, 1.5 μM dexamethasone, 12 μM insulin, 220 μM indomethacin, 1% penicillin / streptomycin, 0.5 mM IBMX under normal conditions (37 ° C, 5% CO 2 ) to continue culturing adipose tissue-derived adipose mesenchymal active cells. After culturing for 7-15 days, take the induced cultured cell clones, fix with methanol for 5 minutes, rinse with 40% alcohol, add oil red O dye to stain for 15 minutes, rinse with 75% alcohol, rinse with water, counterstain with hematoxylin for 1 minute, and observe the staining under a microscope In the case, oil red O staining was positive, indicating that there were lipid vesicles in the cells. According to this feature, it can be judged that the adipose mesenchymal active cells have shown a tendency to differentiate into mature adipocytes. Undifferentiated or partially differentiated adipo...

Embodiment 3

[0049] Embodiment 3: Adipose mesenchymal active cell preparation of the present invention

[0050] Using the adipose-derived mesenchymal active cells extracted in Example 1 as a raw material, according to the adipose-derived mesenchymal active cells: adipose tissue volume ratio (1:20), resuspend the adipose-derived mesenchymal active cells with physiological saline, and form a suspension by blowing evenly. liquid, transfer the cell suspension and filled fat tissue to the syringe respectively, connect the syringe containing the cell suspension and filled fat to the three-way tube, push back and forth until fully mixed, and form the adipose mesenchymal activity of the present invention cell preparations.

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Abstract

The invention discloses an extraction method and application of adipose mesenchymal active cells of an autologous germinal layer. The method comprises the following steps: step A: collecting adipose tissues, mechanically crushing and washing with a normal saline; step B: adding the crushed adipose tissues into a buffering solution and stirring until the solution is clarified; removing damaged tissues, blood and red blood cells; step C: adding the adipose tissues into a mixed enzyme solution of I type collagenase and type II collagenase, which has the mass concentration of 0.20 percent, and uniformly mixing; culturing in a shaking table and centrifuging to obtain precipitated adipose mesenchymal active cells. According to the extraction method and application of the adipose mesenchymal active cells, disclosed by the invention, blood cellular fluid is added into the prepared adipose mesenchymal active cells, so that the proliferation capability of the adipose mesenchymal active cells isrelatively strong; an adipose mesenchymal active cell preparation can be used for a plastic surgery, is especially put into a region under treatment and has good wrinkle-removing and beautifying effects.

Description

technical field [0001] The invention relates to the technical field of medical cosmetology, in particular to a preparation method and application of an adipose mesenchymal active cell preparation. Background technique [0002] Stem cells are a kind of cell population with self-renewal, high proliferation and multi-directional differentiation potential, and are the "ancestor cells" and "universal cells" that form various tissues and organs of the human body. According to their developmental stage, they can be divided into embryonic stem cells and adult stem cells, and according to their differentiation potential, they can be further divided into totipotent stem cells, pluripotent stem cells and unipotent stem cells. [0003] Stem cells can produce and secrete a large number of bioactive factors, which can effectively regulate body cell signal transduction, activate human stem cells, and then physiologically repair or replace damaged, diseased and aging cells in the body. For...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/077A61L27/36A61L27/38
CPCA61L27/3604A61L27/3804C12N5/0653C12N5/0667A61L2430/34C12N2500/38C12N2500/40C12N2501/01C12N2501/33C12N2506/1384C12N2501/855
Inventor 白晋
Owner 白晋
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