Extraction method and application of adipose mesenchymal active cells of autologous germinal layer

An extraction method and germinal layer technology, applied in the field of medical cosmetology, can solve the problems of lack of adipose-derived mesenchymal active cells for beautification and weak proliferative ability of adipose-derived mesenchymal active cells, so as to reduce the formation of melanin, enhance activity, reduce side effects
CN108728409APending Publication Date: 2018-11-02白晋
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
白晋
Publication Date
2018-11-02

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Abstract

The invention discloses an extraction method and application of adipose mesenchymal active cells of an autologous germinal layer. The method comprises the following steps: step A: collecting adipose tissues, mechanically crushing and washing with a normal saline; step B: adding the crushed adipose tissues into a buffering solution and stirring until the solution is clarified; removing damaged tissues, blood and red blood cells; step C: adding the adipose tissues into a mixed enzyme solution of I type collagenase and type II collagenase, which has the mass concentration of 0.20 percent, and uniformly mixing; culturing in a shaking table and centrifuging to obtain precipitated adipose mesenchymal active cells. According to the extraction method and application of the adipose mesenchymal active cells, disclosed by the invention, blood cellular fluid is added into the prepared adipose mesenchymal active cells, so that the proliferation capability of the adipose mesenchymal active cells isrelatively strong; an adipose mesenchymal active cell preparation can be used for a plastic surgery, is especially put into a region under treatment and has good wrinkle-removing and beautifying effects.
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Description

technical field

[0001] The invention relates to the technical field of medical cosmetology, in particular to a preparation method and application of an adipose mesenchymal active cell preparation. Background technique

[0002] Stem cells are a kind of cell population with self-renewal, high proliferation and multi-directional differentiation potential, and are the "ancestor cells" and "universal cells" that form various tissues and organs of the human body. According to their developmental stage, they can be divided into embryonic stem cells and adult stem cells, and according to their differentiation potential, they can be further divided into totipotent stem cells, pluripotent stem cells and unipotent stem cells.

[0003] Stem cells can produce and secrete a large number of bioactive factors, which can effectively regulate body cell signal transduction, activate human stem cells, and then physiologically repair or replace damaged, diseased and aging cells in the body. For...

Claims

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