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Porcine parvovirus (PPV) virus-like particles (VLPs) antibody detection kit as well as preparation method and application thereof

A parvovirus and antibody detection technology, applied in the directions of viruses/phages, biochemical equipment and methods, viruses, etc., can solve the problems of poor immunogenicity of a single protein or polypeptide, and achieve stable results, good specificity and repeatability, Sensitive effect

Inactive Publication Date: 2018-11-09
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the ELISA methods currently used to detect PPV antibodies use inactivated viruses, recombinant viruses, individual proteins or polypeptides as antigens, but there are safety risks in using intact virus particles as antigens, and the immunogenicity of individual proteins or polypeptides is relatively poor

Method used

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  • Porcine parvovirus (PPV) virus-like particles (VLPs) antibody detection kit as well as preparation method and application thereof
  • Porcine parvovirus (PPV) virus-like particles (VLPs) antibody detection kit as well as preparation method and application thereof

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Embodiment Construction

[0052] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the protection scope of the present invention.

[0053] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terminology used in the description of the present invention in this specification is only for the purpose of describing specific embodiments, and is not used to limit the present invention. The term "and / or" used in this specification includes any and all combinations o...

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Abstract

The invention discloses a porcine parvovirus (PPV) virus-like particles (VLPs) antibody detection kit as well as a preparation method and application thereof. The detection kit comprises an enzyme label plate pre-enveloped with PPV VLPs, a blocking solution, sample diluent, an enzyme conjugate, a concentrated washing solution, an enzyme substrate solution and a stop buffer. According to the preparation method, the kit for detecting PPV is established by using the PPV VLPs as an envelope antigen for the first time; the kit also can be used for quickly detecting the antibody level of the PPV inserum; the kit provided by the invention is high in detection sensitivity, good in specificity and reproducibility, and stable in results. Furthermore, the used VLPs are safe and harmless to an operator and the environment, and are free from infectivity. In addition, the kit provided by the invention is applied to the development of an escherichia coli expression system, thus having the advantagesof being economical and inexpensive.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a porcine parvovirus VLPs ELISA antibody detection kit and a preparation method and application thereof. Background technique [0002] Porcine Parvovirus (PPV) is a highly resistant and highly contagious virus, and it is one of the pathogens that cause reproductive disorders in sows. The main clinical features of porcine parvovirus disease are abortion in pregnant sows, Fetal death and mummification. The disease is associated with diseases such as multisystem weakness syndrome and porcine exudative dermatitis in weaned piglets. If susceptible adult pigs are exposed to the presence of PPV during mating or pregnancy, the virus can easily cross the placental barrier and infect embryos. and fetus. The survey found that the disease was distributed globally, especially harmful to pregnant sows and piglets, which hindered the industrialization of pig breeding and caused h...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569
CPCC07K14/005C12N15/70C12N2750/14322C12N2750/14323C12N2800/22G01N33/535G01N33/54306G01N33/56983G01N2333/015
Inventor 郭慧琛孙世琪白满元韩世充董虎宋品魏衍全张韵殷宏
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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