A kind of bacterial agent containing bacillus batavia hj-1 and application thereof

A technology of bacillus and bacterial agent, applied in the field of microbial remediation of heavy metal polluted soil, can solve the problems of damaged soil quality, unstable effect, difficult to reach remediation standards, etc.

Active Publication Date: 2021-03-02
HUNAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Most of the existing remediation technologies use a single organic passivator or inorganic passivator. With the increase of the risk of heavy metal pollution in rice field soil, the remediation effect of a single inorganic or organic passivator is often not ideal. Problems such as instability or damage to soil quality are difficult to meet repair standards

Method used

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  • A kind of bacterial agent containing bacillus batavia hj-1 and application thereof
  • A kind of bacterial agent containing bacillus batavia hj-1 and application thereof
  • A kind of bacterial agent containing bacillus batavia hj-1 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0081] Hydrogen sulfide production experiment of the present invention (lead acetate test paper verification experiment).

[0082] 1. Preliminary determination of hydrogen sulfide production by Bacillus bataviensis HJ-1

[0083] The activated strain HJ-1 was preliminarily screened for hydrogen sulfide production in LB medium by lead acetate test paper.

[0084] Results: The triangular flask was tightly sealed with a ground mouth. After the strain was inserted, it was first cultured on a shaker with a sealing film until the strain grew slightly, and then replaced with a ground sieve under aseptic conditions, and the lead acetate test paper was fixed and hung to continue the culture. The results showed that after overnight cultivation, the lead acetate test paper turned black, showing that the strain could produce hydrogen sulfide, resulting in black lead sulfide. see results figure 1 .

[0085] 2. CdS production experiment after culturing strain HJ-1 in cadmium solution.

...

Embodiment 2

[0088] Fourier transform infrared (FT-IR) spectroscopic analysis of strain HJ-1.

[0089] After the HJ-1 strain was cultured, it was freeze-dried and analyzed by Fourier transform infrared spectroscopy (FT-IR). See image 3 .

[0090] The results show that the wavelength is 3414cm -1 The bands indicate that the strain contains the groups -NH and -OH at wavelengths of 1649, 1545, 1454, 1237 and 1056 cm -1 The bands indicated that the strain contained -NH twist group or carbonyl group. The surface of this strain contains groups such as -NH, -OH and C-O.

Embodiment 3

[0092] Add cadmium (II), lead (II), and arsenic (III) mother solutions to the triangular flasks with sterile medium, so that the final concentrations of cadmium (II), lead (II), and arsenic (III) respectively reach 100 mg / L, under aseptic conditions, were inoculated with Batavia bacillus HJ-1 with 2% inoculum, and the control CK was not inoculated. After inoculation, shake (180rpm) culture at 30°C for 48h, then centrifuge at 6000rpm for 10min, and measure the content of cadmium(II), lead(II) and arsenic(III) in the supernatant respectively. The results are shown in Table 2.

[0093] Table 2. The adsorption effect of strain HJ-1 on cadmium (II), lead (II) and arsenic (III) in culture medium

[0094]

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Abstract

The invention discloses a bacterial agent containing Batavia bacillus HJ‑1 and an application thereof. The newly screened strain Bacillus Batavia HJ‑1 is a versatile and powerful strain. It has multiple functions such as passivation of heavy metals cadmium and / or lead and / or arsenic. The bacterial agent prepared by using this strain can be widely used in light to moderate cadmium, lead, and arsenic polluted paddy fields, and can reduce the soil active cadmium, lead, and arsenic in heavy metal polluted paddy fields by 52.2-53.3%, 51.0-58.1%, and 45.0-45.0%, respectively. 48.8%, the content of cadmium, lead and arsenic in polished rice decreased by 54.2-55.5%, 53.3-55.0% and 47.8-50.0% respectively. At the same time, it also increased the soil pH value by 0.7-1.13. This product has the functions of repairing acidified soil and fertilizing soil It has many functions such as improving the physical and chemical properties of soil, and has great promotion and application value.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of heavy metal-contaminated soil, and in particular relates to a bacterial agent containing Batavia bacillus HJ-1 and its application in lead, cadmium and arsenic compound polluted soil. Background technique [0002] Soil is a precious natural resource and a necessary condition for human survival. With the industrial "three wastes", urban waste, motor vehicle exhaust emissions, sewage irrigation and the use of inferior chemical fertilizers, soil heavy metal pollution is becoming more and more serious. According to the National Soil Pollution Survey Bulletin issued by the Ministry of Environmental Protection and the Ministry of Land and Resources in 2014, the results show that nearly 1.6 million km 2 The land is polluted by heavy metals, and the over-standard rates of Cd, Hg, As, Cu, and Pb are 7.0%, 1.6%, 2.7%, 2.1%, and 1.5%, respectively. [0003] Soil heavy metal pollution not o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/80B09C1/10C12R1/07
CPCB09C1/10B09C2101/00C05B13/02C12N1/20C05G3/80C05D9/00C05F5/008C05F11/00C05F11/02C05F11/08
Inventor 单世平郭照辉吴民熙程伟李一路张敏魏小武
Owner HUNAN INST OF MICROBIOLOGY
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