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Method for detecting content of iohexol in plasma of rhesus monkeys and application thereof to evaluation of influence of drug on GFR (Glomerular Filtration Rate)

A technology of iohexol and rhesus monkeys, which is applied in the field of drug evaluation, can solve the problems affecting the accuracy of glomerular filtration rate and large fluctuations in detection data, and achieves the advantages of method promotion, strong specificity, stability and accuracy Measured effect

Inactive Publication Date: 2018-11-16
SICHUAN PRIMED BIO TECH CO LTD
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Problems solved by technology

In addition, the RSDs of the test samples were all greater than 5%, and the test data fluctuated greatly, which affected the accuracy of glomerular filtration rate evaluation

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  • Method for detecting content of iohexol in plasma of rhesus monkeys and application thereof to evaluation of influence of drug on GFR (Glomerular Filtration Rate)
  • Method for detecting content of iohexol in plasma of rhesus monkeys and application thereof to evaluation of influence of drug on GFR (Glomerular Filtration Rate)
  • Method for detecting content of iohexol in plasma of rhesus monkeys and application thereof to evaluation of influence of drug on GFR (Glomerular Filtration Rate)

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[0060] (1) Preparation of standard solution for standard curve: Weigh 10 mg of iohexol standard product, accurately weigh it, place it in a 10 mL volumetric flask, add methanol to dissolve completely, dilute to the mark with methanol, and mix to obtain 1 mg / mL stock solution. Take the stock solution and dilute it step by step to obtain a series of concentration standard working solutions (1000, 800, 600, 400, 200, 100, 50, 25 μg / mL). The specific method is as follows: Take 1600 μL of 1 mg / mL stock solution and add it to 400 μL methanol, and vortex to obtain 800 μg / mL iohexol working solution; take 1000 μL of 800 μg / mL iohexol working solution and add it to 1000 μL methanol, Vortex and mix to obtain 400 μg / mL iohexol working solution; take 300 μL of 800 μg / mL iohexol working solution and add 300 μL of 400 μg / mL iohexol working solution, and vortex to obtain 600 μg / mL iohexol working solution Hexyl alcohol working solution; add 600 μL of 400 μg / mL iohexol working solution into...

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Abstract

The invention discloses an exclusive method for detecting the content of iohexol in plasma of rhesus monkeys and application thereof to evaluation of the influence of a drug on GFR (Glomerular Filtration Rate), and belongs to the field of drug evaluation. The exclusive method comprises the following steps: (1) standard product working solution and standard curve preparation: preparing standard production working solutions in various concentrations; (2) preparation of an internal standard production solution preparing an iohexol impurity I working solution; (3) sample pretreatment; (4) determination of iodexol: determining under the conditions that a C18 is used as a chromatography column, a mobile phase comprises a mobile phase A which is methanol and a mobile phase B which is phosphoric acid water, isocratic elution with elution time of 10 minutes , 20 to 40 DEG C in temperature and 0.2 to 1.5 mL / min in flowing speed, the detection wavelength of a chromatogram is 190 to 320 nm and thesampling volume is 1 to 20 mu L. The exclusive method disclosed by the invention is capable of detecting the content of the iohexol, is accurate and simple and is time-saving and labor-saving; the dosage of the iohexol required for determining and evaluating the GFR by utilizing the iodexol clearance rate is smaller, and the safety of kidney is greatly improved; the exclusive method is simple andreliable, the exclusiveness is strong, and effective experimental data can be provided for clinical application.

Description

technical field [0001] The invention relates to a method for detecting the iohexol content in rhesus monkey plasma and its application in evaluating the effect of drugs on GFR, in particular to a method for detecting the iohexol content in rhesus monkey plasma, which belongs to the field of drug evaluation. Background technique [0002] In recent years, chronic kidney disease (CKD) has become a serious threat to human health and life. In 2013, 347 million people around the world suffered from diabetes. WHO believes that by 2030, chronic kidney disease will be one of the main diseases leading to human death, causing serious social and economic burdens. The clinical manifestations of CKD are elevated glomerular filtration rate (GFR) and urinary microalbumin. A large number of evidences show that genetic factors, hyperglycemia, hypertension and hyperlipidemia can cause or promote the process of CKD. Although CKD is gradually being recognized as the leading cause of morbidity ...

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 杨真颜曾文龚立杨遵远梁艺男
Owner SICHUAN PRIMED BIO TECH CO LTD
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