Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

PCT-CRP dual card and preparation method thereof

A PCT-CRP, dual card technology, applied in the field of medical examination, can solve the problems of complicated operation and prone to hook effect in CRP

Inactive Publication Date: 2018-11-20
ZYBIO INC
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the patent No. 201210212105.4, a colloidal gold test strip for PCT / CRP joint detection is disclosed. In this patent, the detection of PCT and CRP is carried out by double-antibody sandwich method, but CRP is easy to detect. There is a hook effect, so it is necessary to manually dilute the test sample before the test. The operation is more complicated, and it cannot meet the urgent needs of doctors for the test results. Therefore, it is urgent to develop a fast, sensitive, convenient and easy-to-operate PCT-CRP Dual card, and can be detected without processing serum, whole blood and plasma

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PCT-CRP dual card and preparation method thereof
  • PCT-CRP dual card and preparation method thereof
  • PCT-CRP dual card and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Determination of the concentration of CRP and PCT monoclonal antibody 1 on the detection line of embodiment 1

[0076] 1. Experimental setup

[0077] Prepare anti-PCT, CRP antibody colloidal gold protein complexes according to the above process, evenly coat the colloidal gold protein complexes on the glass fiber, dry and set aside.

[0078] Take procalcitonin monoclonal antibody 1 coating solution and 0.1, 0.5, 1, 1.5, 2, 2.5, Appropriate amount of 3.0, 3.5, 4, 5, 6mg / mL C-reactive protein coating solution, and coat the coating solution on the detection line on the nitrocellulose membrane with a film spotter, the coating rate is 1μL / cm, 45°C dry.

[0079] Assemble the prepared binding pad and the coated nitrocellulose membrane into a large plate, set up a strip cutter to cut the large plate into 3mm wide semi-finished test strips, and use negative and positive reference products for detection. According to the color effect, the streaking effect of different coating c...

Embodiment 2

[0084] Embodiment 2 Determination of the order in which PCT and CRP packets are placed on the detection line

[0085] Reference: Coating CRP on the first detection line on the nitrocellulose membrane, coating PCT on the second detection line, using absorbent pads, binding pads, and sample pads on the bottom liner of the test strip to assemble, PCT / CRP Dilute the reference product with PBS buffer, a total of 8 gradients. Take 80 μL of the sample to be tested for detection, and after 15 minutes, read the signal value through the matching immunoquantitative analyzer.

[0086] Contrast: Coat PCT on the first test line on the nitrocellulose membrane, coat CRP on the second test line, use absorbent pads, binding pads, and sample pads to assemble on the bottom liner of the test strip, and PCT / CRP was diluted with PBS buffer with a reference product, a total of 8 gradients. Take 80 μL of the sample to be tested for detection, and after 15 minutes, read the signal value through the ...

Embodiment 3

[0090] Embodiment 3 A kind of method for preparing PCT-CRP duplex card, comprises the steps:

[0091] Preparation of the first test line, the second test line and the quality control line

[0092] (1) Configure coating buffer: configure 20mM PBS solution, add 0.02g / L sucrose and 0.005g / L sodium azide to the solution;

[0093] (2) Dilute the C-reactive protein to 1 mg / mL with the coating buffer in step (1) to form the first detection line drawing solution;

[0094] (3) Dilute the procalcitonin monoclonal antibody to 1 mg / mL with the coating buffer in step (1) to form the second detection line of the drawing solution;

[0095] (4) Dilute the goat anti-mouse IgG antibody to 1 mg / mL with the coating buffer in step (1) to form a drawing solution for the quality control line;

[0096] (5) Spray the drawing solution of the first detection line, the drawing solution of the second detection line and the drawing solution of the quality control line on the chromatographic membrane resp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a PCT-CRP dual card. The PCT-CRP dual card comprises a test strip substrate and a chromatographic membrane lapped on the test strip substrate, wherein a first detection line, asecond detection line and a quality control line are sequentially arranged on the chromatographic membrane. On one hand, the test strip detects PCT by a double-antibody sandwich method, and on the other hand, the test strip detects CRP by a competition method; sample dilution is not required; through simultaneous adjustment of the positions of the PCT detection line and the CRP detection line in adetection zone, interference of non-specific adsorption can be reduced; through an anti-human erythrocyte antibody in a sample pad, filtration of whole blood is achieved, and detection of a whole blood sample is achieved; in summary, the PCT-CRP dual card provided by the invention is convenient in detection, has a wider detection range, and is fast and sensitive.

Description

technical field [0001] The invention belongs to the field of medical testing, and in particular relates to a PCT-CRP reagent card and a preparation method thereof. Background technique [0002] Procalcitonin (PCT) is a glycoprotein with no hormone activity, consisting of 116 amino acids, with a relative molecular weight of 13KDa and a half-life of 25-30 hours. PCT has extensive and important clinical application value. The content of PCT in normal human body is less than 0.5ng / ml. The level of PCT is elevated. PCT reflects the active degree of systemic inflammatory response. [0003] C-reactive protein (CRP) is an acute phase reaction protein that can react with pneumococcal C polysaccharide to form a complex. The half-life is 19 hours. Serum CRP is synthesized by the liver. The content of CRP in normal people is very low (about 580ng / ml). When inflammatory diseases or tissue damage occur within 6 to 8 hours, the amount of serum or plasma C-reactive protein increases rapi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/4727G01N2333/4737
Inventor 李洪波张浦东秦川
Owner ZYBIO INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com