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A kind of adipose tissue ex vivo preservation solution and preparation method thereof

A technology for in vitro preservation and adipose tissue, which is applied in the field of cell engineering, can solve the problem of insufficient contact with the preservation solution, and achieve the effect of preventing damage

Active Publication Date: 2021-05-11
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides an in vitro preservation solution for adipose tissue, which can effectively solve the problem that the adipose tissue is suspended on the preservation solution and cannot be fully contacted with the preservation solution when the adipose tissue is preserved in vitro

Method used

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  • A kind of adipose tissue ex vivo preservation solution and preparation method thereof
  • A kind of adipose tissue ex vivo preservation solution and preparation method thereof
  • A kind of adipose tissue ex vivo preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] An adipose tissue in vitro preservation solution, the preservation solution contains 1mL of autologous platelet lysate, 2g of sodium hyaluronate, 3mL of human albumin, 1g of Vc, 0.2g of trehalose, 12mL of thermosensitive hydrogel, and 0.5g of penicillin in every 100mL of the preservation solution , streptomycin 0.3g, amphotericin B 0.02g, and the rest of the solute medium is phosphate buffer with a pH value of 7.4.

[0039] Among them, the preparation method of the temperature-sensitive hydrogel is: mix polyacrylamide, methacrylic acid, acrylic acid and chitosan, then add ammonium persulfate, stir evenly, and after standing for 15 hours, wash the reaction product three times to obtain the temperature-sensitive hydrogel. Glue; wherein, the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and ammonium persulfate is 8:1:0.6:0.15:1.

[0040] The preparation method of this preservation solution is:

[0041] Under aseptic conditions, the above-mention...

Embodiment 2

[0045] An adipose tissue in vitro preservation solution, which contains 0.5mL of autologous platelet lysate, 1mL of human serum albumin, 0.5g of Vc, 0.3g of trehalose, 20mL of thermosensitive hydrogel, 0.6g of penicillin, and streptomycin in every 100mL of the preservation solution. 0.5 g, 0.05 g of amphotericin B, and the rest of the solute medium is phosphate buffer solution with a pH value of 7.6.

[0046] Among them, the preparation method of the temperature-sensitive hydrogel is: mix polyacrylamide, methacrylic acid, acrylic acid and chitosan, then add sodium persulfate, stir evenly, and after standing for 15 hours, wash the reaction product three times to obtain the temperature-sensitive hydrogel. Glue; wherein, the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and sodium persulfate is 10:0.5:0.8:0.2:0.6.

[0047] The preparation method of this preservation solution is:

[0048] Under aseptic conditions, the above-mentioned components are unifor...

Embodiment 3

[0052] An adipose tissue in vitro preservation solution, the preservation solution contains 1.5mL of autologous platelet lysate, 5g of sodium hyaluronate, 5mL of human serum albumin, 0.1g of trehalose, 10mL of thermosensitive hydrogel, 0.2g of penicillin, and 0.1 g of amphotericin and 0.01 g of amphotericin B, and the remaining solute medium is phosphate buffer solution with a pH value of 7.2.

[0053] Among them, the preparation method of the temperature-sensitive hydrogel is: mix polyacrylamide, methacrylic acid, acrylic acid, and chitosan, then add lauroyl peroxide, stir evenly, and after standing for 15 hours, wash the reaction product 3 times to obtain temperature-sensitive water. Gel; wherein, the weight ratio of polyacrylamide, methacrylic acid, acrylic acid, chitosan and lauroyl peroxide is 6:0.8:1:0.3:1.

[0054] The preparation method of this preservation solution is:

[0055] Under aseptic conditions, the above-mentioned components are uniformly mixed to prepare an...

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Abstract

The invention discloses an adipose tissue in vitro preservation solution and a preparation method thereof. The preservation solution includes the following components in parts by weight: 0.5-1.5 parts of autologous platelet lysates, 0-5 parts of sodium hyaluronate, 1-5 parts of human albumin, 0-1 part of Vc, and 0.1-0.3 parts of trehalose , 10-20 parts of temperature-sensitive hydrogel, 0.3-1.5 parts of antibacterial drugs, and 80-90 parts of phosphate buffer solution; the preparation method is as follows: under sterile conditions, mix the components according to the formula to prepare the adipose tissue isolate body preservation solution. The preservation solution prepared by the method of the invention can uniformly disperse the adipose tissue into the preservation solution, make the adipose tissue fully contact with the preservation solution, and is beneficial to the long-term storage of the adipose tissue.

Description

technical field [0001] The invention belongs to the technical field of cell engineering, and in particular relates to an adipose tissue in vitro preservation solution and a preparation method thereof. Background technique [0002] Stem cells are a kind of pluripotent cells with self-renewing ability. With the deepening of stem cell research, more and more stem cells from different sources have become research hotspots in various research institutions, such as bone marrow source Mesenchymal stem cells, placenta-derived mesenchymal stem cells, dental pulp-derived mesenchymal stem cells, adipose-derived mesenchymal stem cells, etc. [0003] With the development of adipose tissue engineering, more and more attention has been paid to adipose-derived stem cells (also called adipose-derived vascular stromal cells), which are mainly used in medical cosmetology and other fields. Since Zuk et al. discovered fibroblast stem cells with multiple differentiation potentials in adipose tis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 高雪华孙雯海泉
Owner CHENGDU QINGKE BIOTECH
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