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Extracting method for ursolic acid and application

An ursolic acid extraction technology, applied in the field of natural plant extraction, can solve the problems of long reflux extraction time and large amount of organic solvents, and achieve the effects of shortening extraction time, long extraction time and low extraction efficiency

Active Publication Date: 2018-11-23
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Based on this, it is necessary to provide a kind of ursolic acid and its

Method used

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  • Extracting method for ursolic acid and application
  • Extracting method for ursolic acid and application
  • Extracting method for ursolic acid and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Extraction of ursolic acid crude extract: take 1.0 g Cynomorium powder, mix ethanol with a concentration of 70% by volume and choline chloride-oxalic acid with a concentration of 4% by mass, wherein the solid-to-liquid ratio is 1:20; Ultrasonic extraction, the time of ultrasonic extraction is 20min, the speed of centrifugation is 10000rpm / min, the time is 10min, and the crude extract of ursolic acid is obtained.

[0040] The calculation formula of extraction rate:

[0041] Extraction of ursolic acid: Mix the above-mentioned crude ursolic acid extract with 8% sodium sulfate by mass percentage to obtain a mixed solution, place the mixed solution in a water bath and heat it to 30°C, and adjust the mixing by phosphate buffer The pH in the solution was adjusted to 5 to obtain ursolic acid.

[0042] The calculation formula of extraction efficiency:

[0043] E represents the extraction efficiency, Ct represents the concentration of ursolic acid in the upper phase, Vt repre...

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Abstract

The invention relates to an extracting method for ursolic acid and an application. The extracting method comprises the following steps: S1, taking songaria cynomorium herb and mixing with ethyl alcohol and a deep-eutectic solvent, ultrasonic-extracting, centrifuging, to obtain ursolic acid crude extract liquid; S2, enabling the ursolic acid crude extract liquid to be mixed with inorganic salt, toobtain mixed liquid, and adjusting a temperature and a pH value of the mixed liquid, to obtain the ursolic acid. The method is capable of using a salting out extraction system in a more moderate usingcondition, and reducing a potential risk of organic solvent extraction. In addition, the method is capable of further using the environment-friendly deep-eutectic solvent as an additive, and improving extraction efficiency of a product.

Description

technical field [0001] The invention relates to the technical field of extraction of natural plants, in particular to an extraction method and application of ursolic acid. Background technique [0002] Cynomorium songaricumRupr. is a perennial, all-parasitic seed plant with fleshy, brown plants that mostly parasitize the roots of Nitraria terrestris. Cynomorium is a commonly used traditional Chinese medicinal material, sweet and warm in nature, and has the functions of tonifying kidney and yang, benefiting essence and blood, moistening intestines and laxatives, and nourishing tendons. Studies have shown that Cynomorium has the functions of enhancing immunity, scavenging free radicals, anti-aging, anti-tumor, preventing and treating cardiovascular diseases, anti-stress, anti-gastric ulcer, and promoting sexual maturity of the body. Cynomorium contains flavonoids, triterpenoids, tannins, polysaccharides, amino acids, organic acids, steroids and other active ingredients. Amon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00A61P25/20A61P35/00A61P31/04A61P31/12A61P3/06A61P9/12A61P39/06A61P31/18
CPCA61P3/06A61P9/12A61P25/20A61P31/04A61P31/12A61P31/18A61P35/00A61P39/06C07J63/008
Inventor 谭志坚王朝云易永健周晚来汪洪鹰余旺杨媛茹
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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