An antibacterial peptide from grouper and its application

Abstract

The invention discloses a grouper antimicrobial peptide. The antimicrobial peptide is grouper CD59 protein, and its amino acid sequence is as shown in SEQ ID NO.1, or the amino acid sequence shown in SEQ ID NO.1 is obtained through Substitution, deletion and / or addition of one or more amino acids and / or terminal modifications with equal or higher activity. The antimicrobial peptide of the grouper provided by the present invention can inhibit the growth activity of Vibrio alginolyticus through experiments, and can be applied to the preparation of fish, especially grouper immune preparations or feed additives, and has broad application prospects; The present invention obtains the CD59 gene sequence of the grouper for the first time, which not only enriches the gene pool of the grouper, but also can be applied to the preparation of recombinant proteins, antibacterial and antibacterial additives and fish immune preparations, and is the gene sequence of the grouper Physiological immunity research provides a basis for new practice.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering research and development, and in particular relates to an antimicrobial peptide of grouper and its application. Background technique [0002] Slanted grouper (Epinepheluscoioides) belongs to Perciformes (Perciformes), Serranidae (Serranidae), grouper subfamily (Epinephelinae), grouper (Epinephelus), is a warm water reef fish, in the Indian Ocean Widely distributed in tropical and subtropical waters of the Pacific Ocean. Grouper is one of the famous and precious marine fishes. Its meat is delicious, and it is loved by consumers all over the world, and its economic value is huge. However, due to the current rapid climate change disasters and seriously polluted water quality problems, the morbidity and mortality rates remain high, which has caused serious economic blows to the artificial breeding of grouper. Vibrio alginolyticus is a common pathogenic bacteria. After the grouper is infe...

AI Technical Summary

Problems solved by technology

Although some scholars have studied the CD59 protein in fishes such as zebrafish (Sun et al., 2013), Nile tilapia (Gan et al. 2015), large yellow croaker, rainbow trout and channel catfish (Liu et al, 2007; Papanastasiou et al. al,2007; Yeh et al,2007), but there is no report on the use of the grouper CD59 protein to inhibit the growth of Vibrio alginolyticus

Images