Method and system for phase-contrast imaging of microfluidic channel rotating cells based on LED array

Abstract

The invention discloses a phase-contrast imaging method and system for microfluidic channel rotation cells based on LED arrays, which sequentially include: LED arrays, microfluidic channels, microscopes, and Cmos camera acquisition modules along the transmission direction of the optical path, and also includes rotational cell feature extraction and Identification module, cell rotation measurement module, cell blur removal module, image cell rotation correction module, rotation cell phase contrast imaging module; phase contrast imaging is the most effective method for transparent sample imaging, it can obtain samples that cannot be seen by ordinary intensity imaging The method of the present invention can eliminate the blurring caused by the rapid movement of cells in the microfluidic channel within one exposure time. In this invention method, LED is used as an asymmetric light source to analyze and study the phase contrast imaging of rotating cell images in the microfluidic channel. Under the condition of asymmetrical illumination, all moving cells in the microfluidic channel can be perfectly imaged, assisting our cells Morphological analysis and learning.

Description

technical field [0001] The invention belongs to the technical field of microscopic phase-contrast imaging, and in particular relates to a method and system for phase-contrast imaging of rotating cells in a microfluidic channel based on an LED array. Background technique [0002] Recent impressive growth has occurred in the state-of-the-art of laboratory-on-a-chip (LOC) devices, which allow the realization of compact structures at the microscopic scale, enabling the processing of samples such as reagents, microcells, etc. In this structure, optical microscopy applied to microfluidic devices has become an increasing concern for real-time observation of biochemical processes in microfluidic channels, counting, velocity measurement, etc. [0003] In biology and medicine, there are significant imaging in biological cell sample morphology analysis, structure, identification, etc., and in the analysis of biological samples to miniaturization, detail, living body, movement and the a...

AI Technical Summary

Problems solved by technology

[0004] In the microfluidic channel, most of the suspended sample cells have basically no rotation and movement under high response exposure time, but a small number of cells move too fast and rotate, resulting in the final phase contrast imaging, the rotating cells cannot be accurately carried out. Matching and Correction

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