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Anti-aging ginkgo leaf composition, preparation method and applications thereof

A technology of delaying aging and ginkgo biloba, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of no in-depth research on the structure-activity relationship of functional ingredients, and no in-depth research on the mechanism of action. Improve DPPH scavenging ability, good anti-aging activity, effect of inhibiting skin aging

Pending Publication Date: 2018-11-30
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the physiological effects of Ginkgo biloba extract are closely related to its complex chemical components. The research on its active components at home and abroad mainly focuses on ginkgolides A, B, J and bilobalide, as well as ginkgo biflavanoids and isoginko biloba. Flavonoids, the main efficacy research focuses on inhibiting cell damage, anti-inflammatory and anti-oxidation, neuroprotection and promoting blood circulation, but there is no in-depth study on the structure-activity relationship between functional components and activities
In addition, according to the basis of its efficacy research at home and abroad, Ginkgo biloba, as a plant source for treating cardiovascular and cerebrovascular diseases, has a good prospect in delaying aging, but there is no in-depth research on its mechanism of delaying aging

Method used

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  • Anti-aging ginkgo leaf composition, preparation method and applications thereof
  • Anti-aging ginkgo leaf composition, preparation method and applications thereof
  • Anti-aging ginkgo leaf composition, preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Ginkgo biloba composition and its extraction method

[0039] (1) Ginkgo leaves are dried and pulverized, passed through a 100-mesh sieve, 15 times of 95% ethanol is added for ultrasonic extraction for 1 hour, extracted 3 times, the supernatants are combined and concentrated under reduced pressure at 50° C. to obtain the crude extract of Ginkgo leaves;

[0040] (2) the crude extract of Ginkgo biloba is diluted with 10 times of water, centrifuged, and the supernatant is adsorbed by DM130 macroporous adsorption resin, and 60% ethanol is the eluent to obtain the Ginkgo biloba eluate;

[0041] (3) Ginkgo biloba eluate was sequentially adsorbed with 732 cationic adsorption resin and HPD5000 macroporous adsorption resin, 60% ethanol was used as eluent, and concentrated under reduced pressure.

[0042] Active ingredient and content detection:

[0043] Weigh more than an appropriate amount of extract for redissolution, make it into a concentration of 1mg / mL with me...

Embodiment 2

[0061] Embodiment 2 Ginkgo biloba composition and extraction method thereof

[0062] (1) Ginkgo leaves are dried and pulverized, passed through a 80-mesh sieve, added 12 times of 90% ethanol for ultrasonic extraction for 1.5 hours, extracted twice, combined supernatants and concentrated under reduced pressure at 40°C to obtain the crude extract of Ginkgo leaves;

[0063] (2) the crude extract of Ginkgo biloba is diluted with 8 times of water, centrifuged, and the supernatant is adsorbed by DM130 macroporous adsorption resin, and 55% ethanol is the eluent to obtain the Ginkgo biloba eluate;

[0064] (3) Ginkgo biloba eluate was sequentially adsorbed with 732 cationic adsorption resin and HPD5000 macroporous adsorption resin, 55% ethanol was used as eluent, and concentrated under reduced pressure.

[0065] Active ingredient and content detection: same as Example 1, the results are as follows.

[0066] Lactones

Embodiment 3

[0067] Embodiment 3 Ginkgo biloba composition and its extraction method

[0068] (1) Ginkgo leaves are dried and pulverized, passed through a 120-mesh sieve, added 18 times of 100% ethanol for ultrasonic extraction for 0.5 h, extracted 4 times, combined supernatants and concentrated under reduced pressure at 55° C. to obtain the crude extract of Ginkgo biloba;

[0069] (2) the crude extract of Ginkgo biloba is diluted with 8 times of water, centrifuged, and the supernatant is adsorbed by DM130 macroporous adsorption resin, 65% ethanol is the eluent, and the Ginkgo biloba eluate obtained;

[0070] (3) Ginkgo biloba eluate was sequentially adsorbed with 732 cationic adsorption resin and HPD5000 macroporous adsorption resin, 65% ethanol was used as eluent, and concentrated under reduced pressure.

[0071] Active ingredient and content detection: same as Example 1, the results are as follows.

[0072] Lactones

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Abstract

The invention relates to a cosmetic product effect additive, and particularly provides an anti-aging ginkgo leaf composition, which contains, by weight, 50-95 parts of ginkgo leaf total lactone, 22-60parts of ginkgo leaf total flavone, and less than 0.0035 part of ginkgo leaf ginkgolic acid, wherein the ginkgo leaf total lactone is one or a plurality of materials selected from ginkgolide A, ginkgolide B, ginkgolide C, ginkgolide J and bilobalide, and the ginkgo leaf total flavone is one or a plurality of materials selected from rutin, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, isorhamnetin-3-O-glucoside and myricetin. According to the present invention, the composition can be obtained by mixing the monomers, and can further be obtained by extracting, separating and purifying ginkgo leaves; the effective components in the ginkgo leaves can be effectively enriched through the multi-stage purification with different resins so as to significantly improve the cell aging delaying effect of the composition; and the anti-aging ginkgo leaf composition can be widely used in various cosmetics.

Description

technical field [0001] The invention belongs to cosmetic additives, and relates to a ginkgo leaf composition and its extraction method and application, in particular to a ginkgo leaf composition for delaying aging, its extraction method and its application. Background technique [0002] Ginkgo biloba appeared on the earth as early as 345 million years ago, and it is a relic tree species unique to China in the Quaternary Ice Age. 70% of the resources of Ginkgo biloba are distributed in my country, and more than 50 countries around the world use Ginkgo biloba extract as a source of plant raw materials for the treatment of cardiovascular and cerebrovascular diseases. [0003] The chemical composition of Ginkgo biloba is quite complex. The main active ingredients in Ginkgo biloba are divided into three categories: flavonoids, ginkgolides and ginkgolic acid. At present, more than 34 flavonoids have been isolated from Ginkgo biloba, and more than 70 flavonoids have been initiall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9771A61Q19/08
CPCA61K2800/805A61K2800/82A61Q19/08A61K8/9771
Inventor 易帆李丽董银卯孟宏王笑月
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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