Application of phenylpyruvate in preparation of immunopotentiator

An immune enhancer, phenylpyruvate technology, applied in the application field of phenylpyruvate in the preparation of immune enhancer, can solve problems such as adverse effects, achieve the effects of stimulating lymphocyte transformation, high physiological activity, and broad application prospects

Active Publication Date: 2018-11-30
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved in the present invention is to overcome the adverse effects of the current mainstream market drugs for improving immunity levels, to provide a new application of phenylpyruvate as an immune enhancer, and to broaden the scope of application of phenylpyruvate, in order to apply phenylpyruvate Provide reference in the field of clinical immunotherapy

Method used

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  • Application of phenylpyruvate in preparation of immunopotentiator
  • Application of phenylpyruvate in preparation of immunopotentiator
  • Application of phenylpyruvate in preparation of immunopotentiator

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Effects of adding phenylpyruvate to common diets on the phagocytosis of peritoneal macrophages in mice

[0051] 1. Experimental method

[0052] In order to study the effect of phenylpyruvate on the immune function of mice, C57BL / 6J male mice were used as research objects, divided into 3 groups (1 control group and 2 treatment groups), reared in a single cage, and the number of repetitions was 8 , fed mice with common diets supplemented with 0% (control group), 0.2% and 0.4% phenylpyruvate, respectively, and phagocytized Escherichia coli in vitro by mouse peritoneal macrophages after 6 weeks. Specific steps include:

[0053] (1) Collect primary mouse peritoneal macrophages: wipe the operation table and pipette gun with alcohol cotton to prevent contamination by bacteria. Filter the HBSS (Hank's Balanced Salt Solution, Hank's Balanced Salt Solution) solution with 5% serum for later use. The mice were killed by neck dislocation, and 3 mL of the above HBSS solut...

Embodiment 2

[0058] Example 2 Effects of Adding Phenylpyruvate to Common Diets on the Proliferation of Spleen Lymphocytes in Mice

[0059] 1. Experimental method

[0060] In order to further study the effect of phenylpyruvate on the immune function of mice, C57BL / 6J male mice were used as research objects, divided into 3 groups (control group and 2 treatment groups), reared in a single cage, and the number of repetitions was 8 , mice were fed with normal diets supplemented with 0% (control group), 0.1% and 0.2% phenylpyruvate, respectively, and spleen lymphocyte proliferation test was carried out after 6 weeks. Specific steps include:

[0061] (1) After the mice were killed by neck dislocation, they were soaked in bromogeramine solution until soaked and then taken out. The spleens of the mice were dissected and separated, transferred to a small beaker containing a small amount of Hanks solution, washed 2 to 3 times, and then placed in On the cell sieve in the plate, use a grinding rod to...

Embodiment 3

[0066] Example 3 Effects of Adding Phenylpyruvate on the Wound Healing Ability of Mice in Common Diets

[0067] 1. In this experiment, by studying the effect of phenylpyruvate on tail wound healing of mice, it is used to illustrate the change of phenylpyruvate on the immune function. The specific test method is:

[0068] (1) The experiment took C57BL / 6J male mice as the research object, divided them into 3 groups (control group and 2 treatment groups), raised them in single cages, and the number of repetitions was 8. Added 0% (control group), 0.2 % and 0.4% phenylpyruvate diet fed mice.

[0069] (2) Subtract the tissues 1 mm away from the tail tip of the mice in the two treatment groups and the control group. In order to ensure that the wound surface of the tail tip of each mouse is as similar as possible, the scissors are cut perpendicular to the cut surface to form an incision. , and disinfect the wound with iodine and alcohol after the wound is formed. The wound healing ...

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Abstract

The invention discloses application of phenylpyruvate in preparation of an immunopotentiator. The present invention provides new application of the phenylpyruvate, a pharmaceutically acceptable salt or a prodrug of the phenylpyruvate in preparation of the immunopotentiator, or in preparation of a product for enhancing macrophage phagocytosis and / or promoting lymphocyte proliferation, or in preparation of products that promote wound healing. The invention finds that by adding of the phenylpyruvate to a common diet of mice, the immunity level of the mice can be significantly improved, wound healing is promoted, and the amount of the phenylpyruvate added is small, so that the diet can be directly added to animal drinking water, feeds, food or medicines, and has the advantages such as safeness, effectiveness, and no toxic side effects, the phenylpyruvate can be used in patients with low immune functions, can be used as an immunoadjuvant in therapeutic vaccines, and can be used as the immunopotentiator for wound healing, immunodeficiency treatment, and the like. A basis is provided for development of the phenylpyruvate as the immunoadjuvant or health care products, and the phenylpyruvate has a good prospect of popularization and application.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations. More specifically, it relates to the application of phenylpyruvate in the preparation of immune enhancer. Background technique [0002] The body's immunity is an important ability for the human body to defend against pathogenic invasion and disease. If the immune system, which is the line of health defense, is damaged, it may lead to low immunity, making the human body susceptible to pathogenic infection, various chronic diseases or cancer, mental retardation, slowed healing of windows, and even accelerated aging process. In recent years, with the intensification of economic globalization, the pace of human life has been accelerating. Due to factors such as long-term fatigue work, increased psychological pressure, and irregular life, the number of people with low immunity is increasing year by year. . According to China's big health data in 2015, the cost of treating cardio...

Claims

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Application Information

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IPC IPC(8): A61K31/192A61P17/02A61P37/04A23K20/111A23L33/10
CPCA61P17/02A61P37/04A23K20/111A23L33/10A61K31/192A23V2002/00A23V2200/324A23V2250/02
Inventor 束刚江青艳廖正睿朱晓彤王丽娜王松波袁业现余璐璐
Owner SOUTH CHINA AGRI UNIV
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