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HPLC analysis method for simultaneously determining concentration of curcumin and 5-fluorouracil in blood plasma

A technology for fluorouracil and curcumin, which is applied in the field of HPLC analysis for simultaneous determination of the concentrations of curcumin and 5-fluorouracil in animal plasma, can solve problems such as clinical use limitation, bone marrow suppression, gastrointestinal toxicity, etc., and save analysis time and cost. , improve the stability, the effect of simple pretreatment method

Active Publication Date: 2018-12-07
SOUTHWEST MEDICAL UNIVERISTY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

5-Fluorouracil (5-FU) is a pyrimidine anticancer drug, and it is also a first-line antineoplastic drug used clinically. It has good curative effect on various cancers (such as liver, stomach, pancreatic cancer and breast cancer). However, it has serious adverse reactions such as gastrointestinal toxicity and bone marrow suppression, which limit its clinical use.
[0008] Although compared with the HPLC method, the HPLC-MS / MS method reflects the complementary advantages of chromatography and mass spectrometry, and combines the high separation ability of chromatography for complex samples with high selectivity, high sensitivity and the advantages of providing relative molecular mass and structural information. The advantages of combining, but the biggest disadvantage is that the above analysis methods can only detect the content of curcumin and 5-fluorouracil in different chromatographic systems, but not analyzed together in the same chromatographic system

Method used

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  • HPLC analysis method for simultaneously determining concentration of curcumin and 5-fluorouracil in blood plasma
  • HPLC analysis method for simultaneously determining concentration of curcumin and 5-fluorouracil in blood plasma
  • HPLC analysis method for simultaneously determining concentration of curcumin and 5-fluorouracil in blood plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Determination of curcumin and 5-fluorouracil concentrations in the blood plasma of embodiment 1SD rats.

[0053] 1. Preparation of standard solution

[0054] Preparation of curcumin standard solution: Accurately weigh curcumin standard, dissolve in methanol solution and shake well to obtain 80 μg / ml curcumin standard solution.

[0055] Preparation of 5-fluorouracil standard solution: Accurately weigh 5-fluorouracil standard, dissolve in methanol solution and shake well to obtain 28.2500 μg / ml 5-fluorouracil standard solution.

[0056] The configuration of the mixed standard solution of curcumin and 5-fluorouracil: prepare the CU / 5-FU standard mixed solution according to the CU:5-FU molar ratio of 1:1. Namely: Accurately weigh 2000μg CU and 706.25μg 5-FU into a 50ml volumetric flask, add 50ml of methanol to dissolve, and obtain the mother liquor of CU / 5-FU mixture, in which the concentrations of CU and 5-FU are 40μg / ml and 14.1250μg / ml respectively. ml. Then dilute wi...

Embodiment 2

[0092] Example 2 Measured the metabolism of different ratios of curcumin and 5-fluorouracil mixture in SD rat plasma.

[0093] After SD rats were injected with CU / 5-FU mixture with a molar ratio of 1:1, 1:2, and 1:4 into the tail vein, the content of CU and 5-FU in plasma was detected by this analytical method.

[0094] (1) SD rats were fasted for 12 hours before administration (without water), and administered according to the experimentally designed administration scheme after grouping. After administration, blood was collected according to the sampling time of the experimental design. The blood samples were placed in heparin tubes, the whole blood was centrifuged at 5000rpm / min for 3min, and the supernatant plasma was divided into 100μl and stored in a -80°C refrigerator until assayed.

[0095] (2) Take 100 μL of rat plasma sample, and after processing with the method of “plasma sample pretreatment” described in Example 1, take 100 μL of the supernatant and transfer it int...

Embodiment 3

[0097] Example 3 Determination of the concentrations of curcumin and 5-fluorouracil in rabbit plasma.

[0098] 1. Preparation of standard solution

[0099] Preparation of curcumin standard solution: Accurately weigh the curcumin standard product, dissolve it in methanol solution and shake it well to obtain 80 μg / ml curcumin standard solution.

[0100] Preparation of 5-fluorouracil standard solution: Accurately weigh 5-fluorouracil standard, dissolve in methanol solution and shake well to obtain 28.2500 μg / ml 5-fluorouracil standard solution.

[0101] The configuration of the curcumin / 5-fluorouracil mixture standard solution: prepare the CU / 5-FU standard mixture solution according to the CU:5-FU molar ratio of 1:1. Namely: Accurately weigh 2000μg CU and 706.25μg 5-FU into a 50ml volumetric flask, add 50ml of methanol to dissolve, and obtain the mother liquor of CU / 5-FU mixture, in which the concentrations of CU and 5-FU are 40μg / ml and 14.1250μg / ml respectively. ml. Then dil...

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Abstract

The invention belongs to the technical field of pharmacokinetic analysis, and relates to an HPLC analysis method for simultaneously determining concentration of curcumin and 5-fluorouracil in blood plasma. The method includes the following steps of preparation of a standard substance solution, sample pretreatment, sample separation, detection of a peak area of a sample, and manufacturing of a standard curve. The method overcomes the difficulty that the difference in polarity between the curcumin and the 5-fluorouracil is large, and elution and separation are not easy to perform in a same chromatographic system, and can simultaneously determine the concentration of the curcumin and the 5-fluorouracil in the blood plasma.

Description

technical field [0001] The invention belongs to the technical field of pharmacokinetic analysis, and relates to an analysis and determination method of drugs in vivo, more specifically, relates to an HPLC analysis method for simultaneously determining the concentrations of curcumin and 5-fluorouracil in animal plasma. Background technique [0002] Curcumin (CU) is a phenolic pigment extracted from the rhizomes of ginger plants such as turmeric. Curcumin is an orange-yellow crystalline powder with a slightly bitter taste and is insoluble in water. Modern studies have found that curcumin has the effects of lowering blood fat, anti-tumor, anti-inflammatory, choleretic, and anti-oxidation, and has good practicability and application prospects in the field of anti-cancer. 5-Fluorouracil (5-FU) is a pyrimidine anticancer drug, and it is also a first-line antineoplastic drug used clinically. It has good curative effect on various cancers (such as liver, stomach, pancreatic cancer a...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/74G01N30/88
CPCG01N30/02G01N30/74G01N30/88G01N2030/009G01N2030/027G01N2030/8822
Inventor 赵领皮超魏郁梦徐婷梁靖赵仕杰杨红茹郭璞胡美左英
Owner SOUTHWEST MEDICAL UNIVERISTY
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