Fodder composite selenium-rich probiotic bacillus solid preparation and preparation method thereof

A compound bacillus and bacillus technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of long production cycle, high cost of organic selenium products, difficult large-scale production and popularization and application, etc. Achieve short production cycle, realize industrialized production, and reduce production cost

Inactive Publication Date: 2018-12-11
INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problems in the prior art are as follows: 1. At present, organic selenium products such as selenium-enriched agricultural products have the disad

Method used

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  • Fodder composite selenium-rich probiotic bacillus solid preparation and preparation method thereof
  • Fodder composite selenium-rich probiotic bacillus solid preparation and preparation method thereof
  • Fodder composite selenium-rich probiotic bacillus solid preparation and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0056] The preparation operation steps of a compound selenium-enriched probiotic Bacillus solid preparation for feeding are as follows:

[0057] (1) Activation of strains

[0058] The Bacillus licheniformis and Bacillus subtilis stored at -20 °C were inoculated on the YPD liquid medium with an inoculum of 3%, and cultured at 37 °C for 30-36 h, so that they were continuously activated for 3 to 5 times to obtain activated licheniform spores respectively. Bacillus and activated Bacillus subtilis. Liquid YPD medium is beef extract peptone medium (YPD), ingredients: peptone 10.0g, beef extract 3.0g, sodium chloride 5.0g, distilled water 1000ml; heat to dissolve, adjust pH to 7.0, after packaging, 121 ℃ High pressure steam sterilization for 20min, spare;

[0059] (2) Preparation of seed solution

[0060] The activated Bacillus licheniformis and the activated Bacillus subtilis were transferred to YPD liquid medium respectively, the transfer inoculum was 1%, and cultured at 37°C fo...

Embodiment 2

[0069] The preparation operation steps of a compound selenium-enriched probiotic Bacillus solid preparation for feeding are as follows:

[0070] (1) Activation of strains

[0071] Bacillus licheniformis and Bacillus subtilis stored at -20 °C were inoculated on YPD liquid medium with an inoculum of 5%, cultured at 37 °C for 30-36 h, and activated continuously for 3 to 5 times to obtain activated licheniform spores respectively. Bacillus and activated Bacillus subtilis;

[0072] (2) Preparation of seed solution

[0073] The activated Bacillus licheniformis and the activated Bacillus subtilis were transferred to YPD liquid medium, respectively, with a transfer inoculum of 3%, and cultured at 37 °C for 24 h to obtain Bacillus licheniformis seed liquid and Bacillus subtilis seed liquid, respectively;

[0074] (3) One fermentation

[0075] Add 56.94 mg of anhydrous sodium selenite to 1000 ml of YPD liquid medium and mix well. Mix and inoculate Bacillus licheniformis seed liquid ...

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Abstract

The invention relates to a fodder composite selenium-rich probiotic bacillus solid preparation and a preparation method thereof. Each gram of the composite selenium-rich probiotic bacillus solid preparation contains the following ingredients: Bacillus licheniformis with the content being 9.41'109 CFU/g, Bacillus subtilis with the content being 9.65'10<9> CFU/g, 48.25 microgrammes of organic selenium and 1.83 microgrammes of inorganic selenium. The preparation method of the fodder composite selenium-rich probiotic bacillus solid preparation comprises the following steps of (1) selection of theculture, (2) preparation of a beef extract-peptone medium, (3) activation of the culture and obtaining of seed liquid, (4) first fermentation, (5) secondary fermentation, (6) drying and (7) grinding and packaging. The composite selenium-rich probiotic bacillus preparation is green and environmentally friendly, not only contains the probiotic bacillus, but also is rich in organic selenium, and achieves the effect of joint actions by combining the double effects of organic selenium and the probiotic bacillus.

Description

technical field [0001] The invention belongs to the technical field of feed biological additives, in particular to a compound selenium-enriched probiotic Bacillus preparation for feed which adopts bacterial culture and trace element biotransformation. Background technique [0002] Selenium is an essential trace element for humans and animals, and has many functions such as improving immunity, anti-cancer, anti-free radicals, delaying aging, antagonizing toxic elements, and preventing certain endemic diseases. Selenium deficiency is a worldwide problem. There is a vast low-selenium area from northeast to southwest in China, and the intake of selenium in humans and animals in these areas is generally low. Therefore, selenium supplementation has become a hotspot in human and animal nutrition research. The currently widely used selenium supplement method is to add inorganic selenium. With the in-depth study of selenium, it is found that sodium selenite has low utilization rate...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/125C12R1/10
CPCA23K10/18C12N1/20
Inventor 杨家军徐晓英陈胜吴义景章薇惠文巧
Owner INST OF ANIMAL HUSBANDRY & VETERINARY MEDICINE ANHUI ACAD OF AGRI SCI
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