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A kind of rice spore culture medium and rice spore preparation method for fermenting and producing lovastatin

A lovastatin and culture medium technology, which is applied in the field of rice spore culture medium and rice spore preparation for lovastatin fermentation, can solve the problems of incomplete sterilization, difficult heat transfer, and bacterial contamination, and reduce bacterial contamination The effect of reducing the risk of infection, reducing the probability of infection, and reducing labor intensity

Active Publication Date: 2021-08-27
NEW FOUNDER HLDG DEV LLC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation steps of the method are cumbersome and there is a high risk of bacterial contamination
Because millet has a high bulk density, heat transfer is difficult, and corn steep liquor often contains a large number of bacillus, there is a high risk of incomplete sterilization when used as a solid medium for sterilization, which is very easy to cause bacterial contamination

Method used

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  • A kind of rice spore culture medium and rice spore preparation method for fermenting and producing lovastatin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] This embodiment relates to a kind of culture medium for preparing rice spores, and this culture medium is prepared by the following method:

[0052] A. Pretreatment of rice

[0053] 1) Weigh 200g of japonica rice (W1), add 100ml of purified water and soak for 2 hours;

[0054] 2) Pour the rice and water into the soup pot, stir-fry on the induction cooker, control the heating temperature of the induction cooker to 70°C, and stir-fry until there is no visible flowing water;

[0055] 3) Transfer the rice to the gauze and spread it flat, steam it at 90°C for 20 minutes, loosen it after cooling slightly, and dry the rice to 280g (W2) to obtain a water content of 40% after pretreatment ((W2-W1) / W1 ×100%) of rice;

[0056] B. Preparation of culture medium

[0057] Add 30 g of nutrient solution to the pretreated rice and mix evenly to obtain the culture medium.

[0058] Each 100 g of the nutrient solution includes 10 g of glucose, 10 g of yeast extract, and the balance is pur...

Embodiment 2

[0064] This embodiment relates to a kind of culture medium for preparing rice spores, and this culture medium is prepared by the following method:

[0065] A. Pretreatment of rice

[0066] 1) Weigh 200g of japonica rice (W1), add 140ml of purified water and soak for 6 hours;

[0067] 2) Pour the rice and water into the soup pot, stir-fry on the induction cooker, control the heating temperature of the induction cooker to 130°C, and stir-fry until there is no visible flowing water;

[0068] 3) Transfer the rice to the gauze and spread it flat, steam it at 105°C for 5 minutes, loosen it after cooling slightly, and air-dry the rice to 320g (W2) to obtain a water content of 60% after pretreatment ((W2-W1) / W1 ×100%) rice;

[0069] B. Preparation of culture medium

[0070] Add 10 g of nutrient solution to the pretreated rice and mix evenly to obtain the culture medium.

[0071] Each 100 g of the nutrient solution includes 10 g of glucose, 10 g of yeast extract, and the balance is...

Embodiment 3

[0077] This embodiment relates to a kind of culture medium for preparing rice spores, and this culture medium is prepared by the following method:

[0078] A. Pretreatment of rice

[0079] 1) Weigh 200g of japonica rice (W1), add 120ml of purified water and soak for 4 hours;

[0080] 2) Pour the rice and water into the soup pot, stir-fry on the induction cooker, control the heating temperature of the induction cooker to 100°C, and stir-fry until there is no visible flowing water;

[0081] 3) Transfer the rice to gauze and lay it flat, steam it at 100°C for 10 minutes, loosen it after a little cooling, and dry the rice to 300g (W2) to obtain a water content of 50% after pretreatment ((W2-W1) / W1 ×100%) rice;

[0082] B. Preparation of culture medium

[0083] Add 20 g of nutrient solution to the pretreated rice and mix evenly to obtain the culture medium.

[0084] Each 100 g of the nutrient solution includes 10 g of glucose, 10 g of yeast extract, and the balance is purified ...

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Abstract

The invention relates to a rice spore medium for fermenting and producing lovastatin. The medium is prepared by mixing rice and nutrient solution. Before the rice is mixed with the nutrient solution, the following pretreatment is carried out: 1) soak the rice with purified water; 2) stir-fry the soaked rice and water mixture until no visible flowing water; 3) stir-fry The good rice is steamed and then cooled, and aired until the moisture content in the rice is 40-60%, so as to obtain the pretreated rice. The culture medium of the invention is easy to sterilize, can greatly reduce the risk of bacterial contamination caused by incomplete sterilization of the culture medium, and can ensure the smooth supply of spores required for large-scale industrial continuous fermentation production of lovastatin. Moreover, the culture medium of the invention has simple components, is convenient to prepare, and can produce large amount and high-quality spores.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a rice spore medium for fermenting lovastatin and a preparation method of the rice spore. Background technique [0002] Lovastatin is a hydroxymethylglutaryl coenzyme A reductase inhibitor (HMG-CoA-RI), which competitively inhibits the activity of HMG-CoA reductase and blocks the synthesis of endogenous cholesterol, thereby regulating lipids. Lipid-lowering effect. Lovastatin was first discovered from the fermentation broth of Aspergillus terreus in 1979 and 1980 by Japan's Endo and Albort et al. It was developed by Merck Co of the United States, and it was approved for marketing by FDA in 1987. The first HMG-CoA-RI on the market. Since its launch, lovastatin has become the drug of choice for clinical treatment and prevention of hyperlipidemia, and in recent years, many new pharmacological activities have been discovered, such as anti-cancer, anti-oxidation, and improvement of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/06C12N3/00C12R1/66
CPCC12N3/00C12P17/06
Inventor 陈华桥张葵
Owner NEW FOUNDER HLDG DEV LLC