Two-photon stimulated emission depletion compound microscope

Abstract

The invention provides a two-photon stimulated emission depletion compound microscope. The compound microscope comprises a two-photon imaging unit and an STED imaging unit; the two-photon imaging unitcan be adopted for a relatively thick sample; the STED super-resolution imaging unit can be adopted for a region of interest on the surface layer of the sample; and excitation light and depletion light are accurately overlapped in three-dimensional distribution through light spots focused by an objective lens. The two-photon stimulated emission depletion compound microscope provided by the invention integrates two functions of STED imaging and two-photon imaging, so that the excitation light and the depletion light are accurately overlapped in three-dimensional distribution through the lightspots focused by the objective lens, and a powerful tool is provided for biomedical frontier research.

Description

technical field [0001] The invention relates to the technical field of micro-optical imaging, in particular to a two-photon stimulated emission loss compound microscope. Background technique [0002] The imaging resolution of optical super-resolution microscopy exceeds the limit of optical diffraction, and its imaging resolution is much higher than that of traditional optical microscopy. It is a research hotspot in recent years, and many types have emerged. Stimulated Emission Depletion (STED) microscopy is the far-field optical microscopy that was first proposed and directly overcomes the optical diffraction limit. It is based on laser confocal microscopy imaging. Compared with other types of super-resolution microscopy Microsurgery, with relatively fast imaging speed, can image living cells and detect finer structures in biomedical research. [0003] The resolution of STED imaging is high, but the imaging depth is very shallow. The resolution of two-photon microscopic ima...

AI Technical Summary

Problems solved by technology

[0003] The resolution of STED imaging is high, but the imaging depth is very shallow. The resolution of two-photon microscopic imaging is poor, but because of the use of near-infrared light, the imaging depth is deep. However, there is no one integrated STED imaging and two-photon imaging. Functional compound microscope, two-photon imaging can be used for thicker samples, and STED super-resolution imaging can be used for the area of ​​interest on the surface of the sample

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