Two-photon stimulated emission depletion compound microscope

A technology of stimulated emission loss and microscopy, applied in microscopes, optics, optical components, etc., can solve the problems of shallow imaging depth, poor two-photon microscopy imaging resolution, and compound microscopes that have not yet been given.

Inactive Publication Date: 2018-12-18
SUZHOU GUOKE MEDICAL TECH DEV CO LTD +1
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Problems solved by technology

[0003] The resolution of STED imaging is high, but the imaging depth is very shallow. The resolution of two-photon microscopic imaging is poor, but because of the use of near-infrared light, the imaging depth is deep. However, there is no one integrated STED imaging and two-photon imaging. Functional compound microscope, two-photon imaging can be used for thicker samples, and STED super-resolution imaging can be used for the area of ​​interest on the surface of the sample

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  • Two-photon stimulated emission depletion compound microscope
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  • Two-photon stimulated emission depletion compound microscope

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[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0029] see figure 1 , is a schematic structural diagram of a two-photon stimulated emission depletion compound microscope 10 provided by an embodiment of the present invention, including: a two-photon imaging unit 100 and a STED imaging unit 200 . in:

[0030] The two-photon imaging unit 100 includes: a femtosecond laser 110, a first mirror M1, a second mirror M2, a first dichromatic mirror DM1, a second filter F2, a first lens L1 and a second lens L2 The be...

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Abstract

The invention provides a two-photon stimulated emission depletion compound microscope. The compound microscope comprises a two-photon imaging unit and an STED imaging unit; the two-photon imaging unitcan be adopted for a relatively thick sample; the STED super-resolution imaging unit can be adopted for a region of interest on the surface layer of the sample; and excitation light and depletion light are accurately overlapped in three-dimensional distribution through light spots focused by an objective lens. The two-photon stimulated emission depletion compound microscope provided by the invention integrates two functions of STED imaging and two-photon imaging, so that the excitation light and the depletion light are accurately overlapped in three-dimensional distribution through the lightspots focused by the objective lens, and a powerful tool is provided for biomedical frontier research.

Description

technical field [0001] The invention relates to the technical field of micro-optical imaging, in particular to a two-photon stimulated emission loss compound microscope. Background technique [0002] The imaging resolution of optical super-resolution microscopy exceeds the limit of optical diffraction, and its imaging resolution is much higher than that of traditional optical microscopy. It is a research hotspot in recent years, and many types have emerged. Stimulated Emission Depletion (STED) microscopy is the far-field optical microscopy that was first proposed and directly overcomes the optical diffraction limit. It is based on laser confocal microscopy imaging. Compared with other types of super-resolution microscopy Microsurgery, with relatively fast imaging speed, can image living cells and detect finer structures in biomedical research. [0003] The resolution of STED imaging is high, but the imaging depth is very shallow. The resolution of two-photon microscopic ima...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G02B21/06G02B21/08G02B21/00
CPCG02B21/0032G02B21/0036G02B21/0076G02B21/06G02B21/08G02B21/082G02B2207/114
Inventor 张运海魏通达昌剑杨皓旻季林
Owner SUZHOU GUOKE MEDICAL TECH DEV CO LTD
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