One for detecting al 3+ fluorescent probes and kits
A technology of fluorescent probes and probes, which is applied in the direction of fluorescence/phosphorescence, luminescent materials, measuring devices, etc., and can solve problems such as inability to distinguish trivalent ions and poor selectivity of fluorescent probes
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Embodiment 1
[0035] Al 3+ The configuration method of the ion concentration standard color card is shown in the following steps.
[0036] (1) Clean the bacteria bottle clean.
[0037] (2) Due to the needs of the experiment, pre-match different concentrations of Al 3+ Ions, concentrations from large to small, label paper on the strain bottle.
[0038] (3) Turning a good probe solution (10 -4 MOL / L to 10 -5 Mol / L) Different amounts of Al 3+ Ion, and use the hand to raise the ultraviolet lamp, carefully observe the color of the solution, found that the alpha 3+ The ion concentration is gradually increased, and the blue-green fluorescent of the solution is gradually enhanced, thereby obtaining Al 3+ Ion concentration standard color card.
Embodiment 2
[0040] Probe of the present invention for Al 3+ Ions have good options.
[0041] (1) Wash the required capacity bottle (ultrasound treatment), the capacity bottle is washed, and the flavonoid dye and methanol are formulated into a concentration of 10 in a capacity bottle. -3 Mother liquor of Mol / L, diluted with methanol to concentrate 10 -4 MOL / L to 10 - 5 Mol / L solution.
[0042](2) Preparation of the metal ion solution: Weigh the corresponding inorganic salt, formulated into the concentration required to experiment in the aqueous solution. The seal is stored.
[0043] (3) Put the mixed probe solution into 10 2 ml of bacteria (bacteria bottle is prepared in advance), add a good ion (such as Al) in the bacteria bottle (such as Al 3+ NA + LI + K + Mn 2+ Mg 2+ , Zn 2+ Cu 2+ , PB 2+ Hg 2+ Ni 2+ , CO 2+ , CD 2+ SR 2+ , Cr 3+ , Fe 3+ NO 3 - H 2 PO 4 - , CL - BR - I - , CLO - ), When the drop is added, shake evenly, carefully observe the color of all bacteria bottles, and Al 3+...
Embodiment 3
[0045] Fluorescence emission spectrum for various interfering species reactions in Formula (I)
[0046] like image 3 Down, add 5 equivalent of Al 3+ Promoting the probe (Equation (I)) The methanol solution of the formation of a significant fluorescence enhancement occurs at 462 nm, while the fluorescence at 462 nm was not changed when adding 10 equivalents of other ions. But here you need to explain Zn 2+ , CD 2+ And MG 2+ There is a weaker fluorescence increasing in 482 nm. In addition, it is worth mentioning that this example is for Fe 3+ , Cr 3+ Almost no response, from this point, the synthesized probes designed in this system are better than some reported Al. 3+ The fluorescent probe (which does not distinguish between trivalent ions well), which also reflects the advantages of the present invention.
[0047] In ion competitive experiment, only H 2 PO 4 - The fluorescence of the solution system will be quenched. From this point, the probe (General (I)) is a high selective Al...
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