Fluorescent probe used for detecting Al<3+> and kit
A technology of fluorescent probes and kits, applied in the field of rapid detection and the synthesis of Al3+ fluorescent probes, which can solve the problems of inability to distinguish trivalent ions and poor selectivity of fluorescent probes
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Embodiment 1
[0035] al 3+ The configuration method of the ion concentration standard color card is shown in the following steps.
[0036] (1) Clean the strain bottle for later use.
[0037] (2) Due to the needs of the experiment, different concentrations of Al were prepared in advance 3+ Ions, concentration from large to small, label paper on the strain bottle.
[0038] (3) to the prepared probe solution (10 -4 mol / L to 10 -5 mol / L) was added dropwise with different amounts of Al 3+ Ions, and excited with a portable ultraviolet lamp, carefully observe the color of the solution, found that with the drop of Al 3+ As the ion concentration increases gradually, the blue-green fluorescence of the solution gradually increases, thus obtaining Al 3+ Ion concentration standard color card.
Embodiment 2
[0040] The probes of the present invention are paired with Al 3+ Ions are very selective.
[0041] (1) Wash the volumetric flask to be used in advance (ultrasonic treatment), wash and dry the volumetric flask for use, and mix the flavonoid dye and methanol in the volumetric flask to a concentration of 10 -3 mol / L mother liquor, and diluted with methanol to a concentration of 10 -4 mol / L to 10 - 5 mol / L solution.
[0042] (2) Preparation of the metal ion solution: Weigh the corresponding inorganic salt and prepare it in the aqueous solution to the concentration required for the experiment. Keep it sealed for later use.
[0043] (3) Put the prepared probe solution into ten 2mL strain bottles (the strain bottles were cleaned in advance), and add prepared ions (such as Al 3+ 、Na + , Li + 、K + , Mn 2+ , Mg 2+ , Zn 2+ 、Cu 2+ , Pb 2+ , Hg 2+ 、Ni 2+ 、Co 2+ 、Cd 2 + 、Sr 2+ 、Cr 3+ , Fe 3+ , NO 3 - 、H 2 PO 4 - , Cl - 、Br - , I - , ClO - ), shake evenly after t...
Embodiment 3
[0045] The compound shown in formula (I) is used for the fluorescence emission spectrum of various interfering species reactions
[0046] Such as image 3 shown, adding 5 equivalents of Al 3+ The methanol solution system of the probe (formula (I)) has an obvious fluorescence enhancement phenomenon at 462nm, but when 10 equivalents of other ions are added, the fluorescence at 462nm does not change much. But what needs to be explained here is that Zn 2+ 、Cd 2+ and Mg 2+ There is a weaker fluorescence enhancement at a wavelength of 482nm. In addition, it is worth mentioning that the probe in this case is sensitive to Fe 3+ 、Cr 3+ There is almost no response. From this point of view, the probes designed and synthesized by this system are better than some reported Al 3+ Fluorescent probes (they cannot distinguish trivalent ions very well), which also embodies the advantages of the present invention.
[0047] In ion competition experiments, only H 2 PO 4 - Quenching the f...
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