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SNP markers for improving cotton coat content and identification and breeding methods for high-yielding cotton

A technology for labeling and cotton, which is applied in the fields of biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc. Select the effect of efficiency and accuracy

Active Publication Date: 2021-05-04
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional breeding methods, through years of multi-point trait identification, cultivate breeding materials with higher clothing content, the selection efficiency is low, the breeding period is long, time-consuming and labor-intensive, and it is easily affected by the environment, resulting in misjudgment, resulting in breeding failure

Method used

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  • SNP markers for improving cotton coat content and identification and breeding methods for high-yielding cotton
  • SNP markers for improving cotton coat content and identification and breeding methods for high-yielding cotton
  • SNP markers for improving cotton coat content and identification and breeding methods for high-yielding cotton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Tracking of the pedigree of the female parent of strong dominant hybrid cotton

[0048] The breeding and improvement process of Ekangmian 9 was sorted out, and the complete pedigree improvement information from the introduction of foreign species was obtained ( figure 1 ). During the improvement of the pedigree, the genetic components of the upland cotton lines Jinzi cotton, Daizi cotton 15, Longzi cotton, Dezi cotton, Sizi cotton 2B and Fuzi cotton were collected, and the yield, quality, resistance and environmental adaptation were realized. Synchronized improvement of sex.

[0049] 2. The ecological adaptation improvement process of the Yangtze River Basin

[0050]According to our tracking of the parent breeding process of excellent hybrids, we found that the adaptability of these excellent hybrids to the Yangtze River Basin was mainly inherited from Ejing 1. The earliest origin of Ejing 1 can be traced to sugar loaf in North Carolina (NC), USA. In 1890, King T...

Embodiment 2

[0062] Genealogical Core Material Whole Genome Resequencing

[0063] 1. Genomic DNA extraction and quality testing

[0064] The whole genome DNA (Paterson et al., 1993) of 26 test materials (see Table 1 in Example 1) was extracted by the improved CTAB method, and the samples were detected by agarose electrophoresis, Nanodrop microspectrophotometer and Qubit Fluorometer fluorescence quantitative instrument Purity, integrity, concentration, and DNA quality test results showed that all sample DNA met the requirements for library construction and sequencing. The test results are as follows:

[0065] 1.1 DNA purity and integrity test results

[0066] Agarose electrophoresis: agarose concentration: 1%; voltage: 100v; electrophoresis time: 40min. The result is as image 3 shown.

[0067] 1.2 Purity and concentration test results

[0068] For the samples with qualified agarose detection results, the purity and concentration of the samples were further detected by Nanodrop micro-...

Embodiment 3

[0079] On the basis of 26 pedigree materials, the deep sequencing data of Sizimian 2B was further collected for analysis (SRR5512449) (Fang L et al., 2017). Using 27 family materials as cultivar groups, according to the SNP detection method, the SNP variation in the 27 materials was detected to obtain the population SNP variation. At the same time, the sequencing data of 31 wild cotton varieties were downloaded from the database (Wang M et al., 2017). The SNP variations detected in the above 27 family varieties and 31 wild cotton were used to construct the population phylogenetic tree and analyze the selection and elimination.

[0080] 1. Experimental method

[0081] 1.1 SNP detection method

[0082] SAMtools was used to detect the SNP of the test materials. It mainly includes the following steps:

[0083](1) Prepare the reference genome and its index file: use the faix command in the SAMtools software to establish the reference genome index;

[0084] (2) SNP detection: u...

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Abstract

The invention relates to the field of cotton SNP markers, in particular to the SNP markers for improving the cotton clothing content and the identification and breeding methods of high-yielding cotton. The present invention identifies a SNP marker that significantly improves the traits of clothing by analyzing the genome resequencing data of the excellent parents and their pedigree materials that have significantly improved the traits of clothing. By selecting the favorable allelic variation (GG gene) type) to select materials with high clothing content, which greatly improves the selection efficiency and accuracy, and significantly shortens the cultivation period of high clothing content materials.

Description

technical field [0001] The invention relates to the field of cotton SNP markers, in particular to the SNP markers for improving the cotton clothing content and the identification and breeding methods of high-yielding cotton. Background technique [0002] my country is a big country in raw cotton production, consumption and export, and cotton plays a pivotal role in my country's national economy. Yield is an important economic trait of cotton. Improving cotton yield has always been an important goal of cotton breeding. Among them, lint yield is an important indicator to measure cotton yield, which consists of the number of plants per unit area, the number of bolls per plant, the weight of a single boll, and the percentage of clothing. . The study found that there was a significant positive correlation between clothing content and lint yield, and the clothing content was less affected by the environment and had higher heritability. Breeding practice also shows that the coat ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李威杨代刚王振玉马雄风周晓箭裴小雨刘艳改贺昆仑张飞任中英张文生
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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