Preparation method and detection method of SERS substrate for detection of dopamine in serum
A dopamine and serum technology, applied in the field of SERS substrate preparation, can solve the problems of poor sensitivity and low selectivity, and achieve the effect of simple pretreatment method, improved sensitivity and enhanced SERS detection signal
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[0029] Step one, Fe 3 o 4 Preparation of micro-nanosphere template:
[0030] 1.35g FeCl 3 ·6H 2 O and 1.0 g of polyethylene glycol (relative molecular mass: 10,000) were dissolved in 40 mL of ethylene glycol, magnetically stirred at room temperature, and then 3.6 g of sodium acetate was added to dissolve completely, and the above mixture was transferred to a reaction kettle at a constant temperature of 200 ° C The reaction was carried out for 8 hours, and the obtained product was washed twice with water and ethanol successively, dried at 60° C. for 8 hours, and then used for later use. Get Fe 3 o 4 The diameter of the micro-nanosphere is about 300nm.
[0031] Step 2, the seed growth method to synthesize gold nanoparticles:
[0032] 1) The preparation method of gold nano-seeds is: 99mL secondary water and 1mL chloroauric acid (mass fraction is 1%) successively join in the three-neck flask of 250mL, after 140 ℃ of oil bath heating 1 hour, add 4mL sodium citrate (mass frac...
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