Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and detection method of SERS substrate for detection of dopamine in serum

A dopamine and serum technology, applied in the field of SERS substrate preparation, can solve the problems of poor sensitivity and low selectivity, and achieve the effect of simple pretreatment method, improved sensitivity and enhanced SERS detection signal

Active Publication Date: 2020-09-22
HEFEI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a surface-enhanced Raman scattering substrate capable of magnetically separating and purifying dopamine in human serum and its preparation method. Also provided is a SERS detection method that uses a magnetically separable SERS substrate to achieve selective and highly sensitive detection of dopamine molecules in human serum that has undergone simple pretreatment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and detection method of SERS substrate for detection of dopamine in serum
  • Preparation method and detection method of SERS substrate for detection of dopamine in serum
  • Preparation method and detection method of SERS substrate for detection of dopamine in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Step one, Fe 3 o 4 Preparation of micro-nanosphere template:

[0030] 1.35g FeCl 3 ·6H 2 O and 1.0 g of polyethylene glycol (relative molecular mass: 10,000) were dissolved in 40 mL of ethylene glycol, magnetically stirred at room temperature, and then 3.6 g of sodium acetate was added to dissolve completely, and the above mixture was transferred to a reaction kettle at a constant temperature of 200 ° C The reaction was carried out for 8 hours, and the obtained product was washed twice with water and ethanol successively, dried at 60° C. for 8 hours, and then used for later use. Get Fe 3 o 4 The diameter of the micro-nanosphere is about 300nm.

[0031] Step 2, the seed growth method to synthesize gold nanoparticles:

[0032] 1) The preparation method of gold nano-seeds is: 99mL secondary water and 1mL chloroauric acid (mass fraction is 1%) successively join in the three-neck flask of 250mL, after 140 ℃ of oil bath heating 1 hour, add 4mL sodium citrate (mass frac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
sizeaaaaaaaaaa
Login to View More

Abstract

A SERS substrate capable of magnetically separating and purifying dopamine in human serum, a preparation method, and a detection method using the substrate relate to the technical field of surface-enhanced Raman scattering technology and related detection methods. The SERS substrate consists of magnetic Fe3O4 micro-nanospheres and gold nanoparticles modified on the surface; charge transfer can occur between Fe3O4 in the substrate and the dopamine to be tested to form a complex to produce strong plasmon resonance; at the same time, Fe3O4 and gold can generate strong electric fields under Ramanexcitation illumination to enhance the SERS signal of dopamine. The pretreated human serum is mixed with the SERS substrate, the silicon wafer is placed in the mixed solution and separated by a magnet, the obtained silicon wafer contains the magnetic substrate and the dopamine molecule of the complex formed with the iron ions in the substrate, and a SERS detection is performed on the silicon waferto obtain a SERS spectrum of the dopamine molecule. Therefore, the sensitivity of the detection results is high.

Description

technical field [0001] The invention relates to the technical field of surface-enhanced Raman scattering technology and related detection methods, in particular to a preparation method and a detection method of a SERS substrate for detecting dopamine in serum. Background technique [0002] Dopamine is an indispensable hormone substance for the human body. It is one of the important neurotransmitters in the brain synthesized and secreted by dopaminergic neurons, and it has a regulatory effect on the emotions and behaviors of the human body. Studies have shown that the concentration imbalance of dopamine can lead to a variety of neurotic diseases: such as Parkinson's disease, schizophrenia and essential hypertension, so its concentration level in human body fluids has become a key factor in the diagnosis of these diseases in internal medicine diagnostics. One of the basis. [0003] Surface-enhanced Raman scattering (SERS) has become a biosensing analysis tool with high sensit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 吴义平李盼邓崇海汪欣程正忠
Owner HEFEI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products