32results about How to "Effective extraction and separation" patented technology

The invention discloses a method for extracting a cesium salt from brine. The cesium salt extraction method comprises the following steps: mixing the brine with an adsorbent, taking out the adsorbent after complete adsorption, and desorbing the adsorbent by adopting a desorption agent to obtain a first pregnant solution; carrying out evaporative concentration on the first pregnant solution, carrying out cooling crystallization, and separating to obtain a first crystal solid and a second pregnant solution; and adjusting the alkalinity of the second pregnant solution, carrying out fractioning extraction, separating to obtain a loaded organic phase and a raffinate, stripping the loaded organic phase by using a stripping agent to obtain a stripping solution which is a cesium salt solution. The invention also discloses a method for extracting a rubidium salt. Compared with traditional methods for extracting the rubidium salt and the cesium salt from the brine, the methods disclosed in the invention adopting adsorption enrichment, fractioning extraction and stripping to obtain the cesium salt solution and a rubidium salt solution have the advantages of simple process, effective extraction and separation of the rubidium salt and the cesium salt, and prevention of the loss of the rubidium salt and the cesium salt.

The invention relates to a phosphine oxide-modified pillar (5) arene derivative and an application thereof, belonging to the technical field of selective separation and extraction agents for thorium (IV) and uranium (VI) from rare earth elements. The phosphine oxide-modified pillar (5) arene derivative is diphenyl phosphine oxide-substituted pillar (5) arene, is used as an extraction agent for selectively separating thorium and uranium from rare earth elements, and can realize the selective separation of uranium to thorium in actinide elements to a certain extent. The extraction agent, namely the phosphine oxide-modified pillar (5) arene in the invention has the advantages of single composition, good chemical stability, simple synthesis and easiness in obtainment, and is suitable for industrial production; furthermore, the using quantity of the extraction agent is low, and the thorium and the uranium can be selectively separated from the rare earth elements under high acidity; and in addition, the extraction agent further has the advantages of high extraction speed, high phase splitting speed and the like. The product disclosed by the invention has great economic benefits and practical application prospects.

The invention discloses a multi-step swelling and polymerization-solid-phase extraction method for extracting genistein from plant nepeta japonica maxim, which comprises the following steps: (1) a multi-step swelling and polymerization method is adopted to prepare a genistein molecularly imprinted polymer with uniform size; (2) a genistein molecularly imprinted solid-phase extraction post is prepared; (3) and the genistein molecularly imprinted solid-phase extraction post extracts and separates the genistein in plant nepeta japonica maxim. The novel globular molecule imprinted polymer with uniform grain diameter (3 to 5 mum) has high selectivity to the genistein. The obtained polymer is prepared into the solid-phase extraction post which can be used for extraction, separation and purification of the genistein in the plant nepeta japonica maxim to obtain products with higher purity. The operation process is simple with greatly reduced use amount of solvent, so that the extracting process is safer and more environmental-friendly, and the purpose of impurity removal, purifying and enriching target genistein can be rapidly achieved.

The invention discloses a method for preparing high-purity norbixin, which comprises the following steps of: adding annatto seeds into aqueous solution of ethanol or acetone, stirring the solution, and performing solid-liquid separation to obtain bixin extract; enriching and desorbing the extract through adsorbent resin; and concentrating the desorption solution, drying the concentrate to obtain bixin crystal, adding aqueous solution of ethanol and alkali into the bixin crystal to dissolve the crystal, adjusting the pH value, separating out norbixin crystal, and performing solid-liquid separation to obtain the high-purity norbixin. Most impurities with high polarity can be removed by adopting macroporous absorption resin enrichment so as to improve the clarity and quality of the extract. Because a discontinuous gradient desorption method is adopted in the desorption process, a low-concentration ethanol flushing resin column can remove polysaccharide and high-polarity impurities; and the adopted high-concentration ethanol desorption greatly improves the quality of the desorption solution and is favorable for subsequent crystallization. By detecting, the HPLC content is more than 95percent, and the yield reaches over 80 percent; and the method provides safer technical guarantee for producing medicinal raw materials, has low extraction cost and is suitable for industrialized production.

The invention discloses a novel technology for extracting threonine. Animal hair is used as raw materials, the animal hair is washed and cleaned up, immersed in 1%-2% of sodium hydroxide solutions for 4 hours and filtered, and filter liquor is used for preparing soluble protein. Acidolysis is conducted on solid substances in 5%-20% hydrochloric acid for 20-60 minutes, and the solid substances are stirred continuously. Precipitate is filtered out after standing for 60 minutes, and the threonine id obtained after the precipitate is dried.

The invention provides an oil extraction process for camellia oleifera seeds, belonging to the field of oil extraction. The oil extraction process for the camellia oleifera seeds comprises the following steps: collection and storage of the camellia oleifera seeds, preliminary pretreatment, secondary pretreatment, redrying, cold pressing, oil-residue separation, degumming, dewaxing, deodorizing and physical deacidifying. According to the invention, through a unique production process, degumming, dewaxing, deodorizing and deacidifying of crude oil are realized, so precious skin-protection components like squalene, Vitamin E (VE) and phytosterol are effectively retained, and natural skin-moisturizing and skin-beautifying functions are obtained; meanwhile, beneficial substances in the crude oil may not be destroyed in the process of extraction.

The invention discloses a method for preparing high-purity annatto. The method comprises the following steps of: adding annatto seeds to an alcohol or acetone aqueous solution; stirring at 30-60 DEG C and separating solid and liquid to obtain an annatto extracting solution; enriching and desorbing the annatto extracting solution through absorption resin; and decompressing, concentrating, crystallizing and drying an obtained desorbing solution to obtain an annatto crystal. In the invention, because macroporous absorption resin is adopted to enrich the annatto, most high-polarity impurities canbe removed, therefore, the clarity and the quality of the extracting solution are improved; a discontinuous gradient desorbing method is adopted in the desorbing process, and a low-concentration alcohol sluicing resin column can be used for removing most polysaccharides and strong-polarity impurities adsorbed by the resin; and then the high-concentration alcohol is used for desorbing to ensure that the desorbing solution quality is greatly improved, which is beneficial to subsequent crystallization. An obtained product has high purity, wherein shown as detection, the content of HPLC (High Performance Liquid Chromatography) is larger than 95 percent, and the yield reaches higher than 80 percent, thus the invention provides a safer technical guarantee for producing officinal grade raw materials, has low extracting cost and is suitable for industrial production.

The invention discloses a method for extracting a-arbutin from fermentation liquor based on membrane separation technology. The method comprises the following steps: (1) microfiltering fermentation liquor; (2) adsorbing and desorbing microfiltrate; (3) concentrating and decolorating desorbed solution; and (4) concentrating and catalyzing the decolorated solution. The ceramic membrane separation technology is applied into the pretreatment of the a-arbutin fermentation liquor, and the method is simple and convenient to operate, high in safety coefficient, low in production cost, high in yield and good in purity and quality compared with the method of flocculating and extracting arbutin in the fermentation liquor with alcohol.

The invention relates to a method for extracting and separating a compound ethyl-alpha-D-furan arabinose from a Nigella sativa seed, which comprises the following steps: selecting a dry Nigella sativaseed as a raw material, pulverizing, degreasing with petroleum ether, extracting with ethanol at room temperature, adding methanol into the extract, dissolving, stirring with silica gel, loading, eluting with dichloromethane and methanol to obtain seven components Fr1-Fr7, and continuously separating and purifying to obtain a compound pure product. The method disclosed by the invention is beneficial to better development and utilization of the medicinal plant, namely the Nigella sativa, and is beneficial to sustainable utilization of the plant; the extraction process is simple, the ethyl-alpha-D-furan arabinose compound monomer is easy to obtain, the solvent can be recycled, the extraction amount is large, and industrial production can be achieved.

The invention relates to application of a cyclophane amide compound containing three central hydrogen bonds as an extracting agent in separation of rare-earth elements, wherein, the cyclophane amide compound containing the three central hydrogen bonds is applied to the separation of the rare-earth elements. The cyclophane amide extracting agent containing the three central hydrogen bonds has single composition, good chemical stability and less consumption; the extracting agent can carry out extraction in neutral solution; the dissolubility of the extracting agent in different solvents can be adjusted through an R group; and the extracting agent further has the advantages of fast extraction speed, fast phase separation speed and the like while extracting the rare-earth elements. Therefore, the cyclophane amide containing the three central hydrogen bonds is taken as a novel extracting agent for extracting and separating the rare-earth elements has practical application prospect.

The invention discloses a composition for stably and efficiently regulating the immune function, which comprises the following raw materials in parts by weight: 50 to 1000 parts of pollen, 5 to 40 parts of vitamin C and 0.5 to 5 parts of lycopene. In the preparation process of the composition, firstly, lipid separation is carried out on the pollen to obtain degreased pollen and an oily component; then separation is carried out by utilizing the degreased pollen to obtain a pollen cytocyst shell material and a pollen polysaccharide and protein mixed component; and finally, the lycopene is dissolved by using the oily component, then the obtained product is mixed with thepollen polysaccharide and protein mixed component and the vitamin C to prepareemulsion, and emulsion nanoparticles are loaded into the pollen cytocyst shell material to carry out microencapsulation recombination embedding. Moreover, the invention discloses a specific preparation method and application of the composition. The composition disclosed by the invention is high in stability and high in delivery efficiency, and can effectively improve the immune function.

The invention relates to a phosphine oxide-modified pillar (5) arene derivative and an application thereof, belonging to the technical field of selective separation and extraction agents for thorium (IV) and uranium (VI) from rare earth elements. The phosphine oxide-modified pillar (5) arene derivative is diphenyl phosphine oxide-substituted pillar (5) arene, is used as an extraction agent for selectively separating thorium and uranium from rare earth elements, and can realize the selective separation of uranium to thorium in actinide elements to a certain extent. The extraction agent, namely the phosphine oxide-modified pillar (5) arene in the invention has the advantages of single composition, good chemical stability, simple synthesis and easiness in obtainment, and is suitable for industrial production; furthermore, the using quantity of the extraction agent is low, and the thorium and the uranium can be selectively separated from the rare earth elements under high acidity; and in addition, the extraction agent further has the advantages of high extraction speed, high phase splitting speed and the like. The product disclosed by the invention has great economic benefits and practical application prospects.

The invention provides an in-vivo biological analysis method for a liposome drug. The total content of the liposome drug is detected by adopting a liquid-liquid extraction method and LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry); a solid-phase extraction method, a liquid-liquid extraction method and LC-MS / MS are adopted to detect the content of the liposome wrapped medicine; and the content of the free liposome drug is obtained by subtracting the content of the liposome-coated drug from the total liposome drug content. According to the invention, after the liposome drug enters the body, the in-vivo free drug and the liposome wrapping part are effectively measured, and reference is provided for the in-vivo pharmacokinetic behavior of the liposome and the in-vivo release degree of the preparation. Compared with a traditional method for measuring the sum of the in-vivo lipidosome and the free medicine through protein precipitation, the method has the advantage that the release degree of the in-vivo lipidosome can be reflected more accurately.

The invention discloses a method for extracting a-arbutin from fermentation liquor based on membrane separation technology. The method comprises the following steps: (1) microfiltering fermentation liquor; (2) adsorbing and desorbing microfiltrate; (3) concentrating and decolorating desorbed solution; and (4) concentrating and catalyzing the decolorated solution. The ceramic membrane separation technology is applied into the pretreatment of the a-arbutin fermentation liquor, and the method is simple and convenient to operate, high in safety coefficient, low in production cost, high in yield and good in purity and quality compared with the method of flocculating and extracting arbutin in the fermentation liquor with alcohol.

The invention discloses a steady-state high-efficiency immune function regulating composition, which comprises the following raw materials in parts by weight: 50-1000 parts of pollen, 5-40 parts of vitamin C, and 0.5-5 parts of lycopene; when the composition is prepared Lipid separation of pollen is carried out first to obtain defatted pollen and oily components; then the pollen cell capsule material and mixed components of pollen polysaccharide and protein are obtained by separating defatted pollen; finally, oily components are used to dissolve lycopene, and then combined with pollen polysaccharides It is mixed with protein mixing components and vitamin C to make an emulsion, and the emulsion nanoparticles are loaded into the shell material of pollen cells for microencapsulation, recombination and embedding. And the specific preparation method and application of the composition are disclosed. The composition of the invention has strong stability, high delivery efficiency and can effectively improve immune function.

The invention discloses a method for preparing high-purity norbixin, which comprises the following steps of: adding annatto seeds into aqueous solution of ethanol or acetone, stirring the solution, and performing solid-liquid separation to obtain bixin extract; enriching and desorbing the extract through adsorbent resin; and concentrating the desorption solution, drying the concentrate to obtain bixin crystal, adding aqueous solution of ethanol and alkali into the bixin crystal to dissolve the crystal, adjusting the pH value, separating out norbixin crystal, and performing solid-liquid separation to obtain the high-purity norbixin. Most impurities with high polarity can be removed by adopting macroporous absorption resin enrichment so as to improve the clarity and quality of the extract. Because a discontinuous gradient desorption method is adopted in the desorption process, a low-concentration ethanol flushing resin column can remove polysaccharide and high-polarity impurities; and the adopted high-concentration ethanol desorption greatly improves the quality of the desorption solution and is favorable for subsequent crystallization. By detecting, the HPLC content is more than 95 percent, and the yield reaches over 80 percent; and the method provides safer technical guarantee for producing medicinal raw materials, has low extraction cost and is suitable for industrialized production.

The invention belongs to the technical field of plant extraction, and particularly relates to an extraction and separation method of nicotiflorin in rose flower. The method comprises the following steps: 1) taking dried rose flowers, pulverizing, degreasing the material with petroleum ether at room temperature, then adding ethanol to extract by cold soaking, and extracting an extract to obtain a total ethanol extract; 2) dissolving the total ethanol extract by methanol and mixing the materials, eluting a mixture through water, 20% ethanol, and 40% ethanol in order through a D101 macroporous resin column, and then drying a solvent to obtain a 40% ethanol elution component; 3) performing gradient elution on the 40% ethanol elution component with dichloromethane-methanol by normal pressure silica gel column chromatography, and combining the materials by silica gel thin layer chromatography to obtain 12 components which are marked as component Fr. 4-1 to Fr. 4-12 according to the polarityof the obtained components from small to large; and 4) obtaining the eleventh component Fr. 4-11 by gel column chromatography and high performance liquid chromatography. The method of the invention can quickly and effectively extract and isolate the nicotiflorin contained in the rose flower, and the extraction yield of the nicotiflorin is high.

The invention discloses a multi-step swelling and polymerization-solid-phase extraction method for extracting genistein from plant nepeta japonica maxim, which comprises the following steps: (1) a multi-step swelling and polymerization method is adopted to prepare a genistein molecularly imprinted polymer with uniform size; (2) a genistein molecularly imprinted solid-phase extraction post is prepared; (3) and the genistein molecularly imprinted solid-phase extraction post extracts and separates the genistein in plant nepeta japonica maxim. The novel globular molecule imprinted polymer with uniform grain diameter (3 to 5 mum) has high selectivity to the genistein. The obtained polymer is prepared into the solid-phase extraction post which can be used for extraction, separation and purification of the genistein in the plant nepeta japonica maxim to obtain products with higher purity. The operation process is simple with greatly reduced use amount of solvent, so that the extracting process is safer and more environmental-friendly, and the purpose of impurity removal, purifying and enriching target genistein can be rapidly achieved.

The invention discloses a novel technology for extracting leucine. According to the novel technology for extracting the leucine, animal hair is used as a raw material. The novel technology for extracting the leucine includes the following steps: firstly, the animal hair is washed cleanly and then soaked in sodium hydroxide solution with the concentration of 1-2% for four hours; and secondly, the animal hair and the sodium hydroxide solution are filtered to obtain filtrate, and the filtrate is used for preparing soluble protein. Solid matter is subjected to acidolysis for 20 to 60 minutes in hydrochloric acid with the concentration of 5-20%, the solid mater and the hydrochloric acid are stirred continuously, after standing for 60 minutes, sediment in the solid matter and the hydrochloric acid is filtered to obtain filtrate, and then the filtrate is dried to obtain the leucine.

The invention discloses a novel process of extracting tryptophan. Animal hair is used as raw materials, the animal hair is cleaned up and soaked in 1-2% of sodium hydroxide solutions for four hours and filtered, wherein filtrate is used for preparing soluble protein. Acidolysis is conducted on solid substances through 5-20% of hydrochloric acid for 20-60 minutes, and the solid substances are constantly stirred. After standing for 60 minutes, precipitates are filtered. After the precipitates are dried, the tryptophan is formed.

The invention relates to application of a cyclophane amide compound containing three central hydrogen bonds as an extracting agent in separation of rare-earth elements, wherein, the cyclophane amide compound containing the three central hydrogen bonds is applied to the separation of the rare-earth elements. The cyclophane amide extracting agent containing the three central hydrogen bonds has single composition, good chemical stability and less consumption; the extracting agent can carry out extraction in neutral solution; the dissolubility of the extracting agent in different solvents can be adjusted through an R group; and the extracting agent further has the advantages of fast extraction speed, fast phase separation speed and the like while extracting the rare-earth elements. Therefore,the cyclophane amide containing the three central hydrogen bonds is taken as a novel extracting agent for extracting and separating the rare-earth elements has practical application prospect.

The invention discloses a method for preparing high-purity annatto. The method comprises the following steps of: adding annatto seeds to an alcohol or acetone aqueous solution; stirring at 30-60 DEG C and separating solid and liquid to obtain an annatto extracting solution; enriching and desorbing the annatto extracting solution through absorption resin; and decompressing, concentrating, crystallizing and drying an obtained desorbing solution to obtain an annatto crystal. In the invention, because macroporous absorption resin is adopted to enrich the annatto, most high-polarity impurities canbe removed, therefore, the clarity and the quality of the extracting solution are improved; a discontinuous gradient desorbing method is adopted in the desorbing process, and a low-concentration alcohol sluicing resin column can be used for removing most polysaccharides and strong-polarity impurities adsorbed by the resin; and then the high-concentration alcohol is used for desorbing to ensure that the desorbing solution quality is greatly improved, which is beneficial to subsequent crystallization. An obtained product has high purity, wherein shown as detection, the content of HPLC (High Performance Liquid Chromatography) is larger than 95 percent, and the yield reaches higher than 80 percent, thus the invention provides a safer technical guarantee for producing officinal grade raw materials, has low extracting cost and is suitable for industrial production.

The invention discloses a solvent separation method for a coating and soft rubber. The method comprises steps as follows: (1) a soft rubber sample sheared into a piece is weighed; (2) the sample is placed in a paint coating dissolution extraction device and an extracting agent is added for dissolution; (3) the paint coating dissolution extraction device is placed in an ultrasonic water bath for ultrasonic treatment, and an extracted sample and an extracting agent solution with paint dissolved are obtained; (4) the extracted sample is weighed; (5) the extracting agent solution is evaporated to dryness and placed in a graphite digester, aqua regia is added and evaporated to dryness, and separated paint is obtained; (6) the paint is cooled to the room temperature to be measured after volume metering. With the adoption of the method, solvent extraction separation can be performed on the paint coating and the soft rubber effectively, independence of the two different substances, namely, the paint coating and the soft rubber, can be guaranteed, and the detection requirement for plastic toys at home and abroad can be met completely. The method has the advantages that the process is simple, the operation is convenient, secondary pollution can be avoided and the like.

The invention provides a method using rose fermentation to prepare fragrant rose wine and the wine prepared by the method and belongs to the technical field of wine processing. The method includes thesteps of firstly, preparing a fermentation base fluid containing a rose fragrance component, wherein the refraction sugar content of the fermentation base fluid is 10-30%; secondly, inoculating yeastinto the fermentation base fluid to perform alcoholic fermentation for 5-7 days; thirdly, during the alcoholic fermentation, extracting the rose fragrance component in the fermentation base fluid, and guiding the rose fragrance component into a wine body to obtain the fragrant rose wine. The method has the advantages that rose fragrance and wine fragrance can be synergistically fused, the original wine body structure and the fine and smooth and mellow taste of the wine are kept, and the method is simple in process flow and operation, capable of achieving year-round production and good in actual application value.

The invention relates to a method for separating and extracting phenolic glycoside compounds from Nigella sativa seeds. The method comprises the following steps that dry Nigella sativa seeds are pulverized, degreasing is carried out with petroleum ether, extracting is carried out with 60-80% ethanol, and a solvent is recycled under reduced pressure to obtain an extract; 2) the extract is dissolvedwith methanol, the extract is mixed with a silica gel column, and gradient elution is carried out with dichloromethane methanol to obtain seven components Fr1-Fr7; 3) silica gel column chromatographyseparation is performed on a fourth component Fr4 to obtain five components (4-1)-(4-5); and 4) gel column chromatography and silica gel column chromatography separation are performed on a component4-3 to obtain 3,5-dihydroxyphenylethanol-3-O-beta-pyranoside, and reversed-phase column chromatography and Sephadex LH-20 sephadex column chromatography separation are performed on a component 4-2 toobtain broadleaf chrysanthemum glycoside C. The method is simple in extraction process, a product is easy to obtain, the solvent can be recycled, the extraction amount is large, and industrial production can be realized.

The invention discloses a novel process of isoleucine extracting. Animal hair is used as raw materials, and the animal hair is cleaned and soaked in 1%-2% sodium hydroxide solutions for four hours and filtered. Filtered liquor is used for preparing soluble protein. Acid hydrolysis is carried out on solid substances for 20-60 minutes by using of 5%-20% hydrochloric acid, the solid substances and the hydrochloric acid are continuously stirred, precipitate is filtered out after standing for 60 minutes, and isoleucine is obtained after the precipitate is dried.

A SERS substrate capable of magnetically separating and purifying dopamine in human serum, a preparation method, and a detection method using the substrate relate to the technical field of surface-enhanced Raman scattering technology and related detection methods. The SERS substrate consists of magnetic Fe3O4 micro-nanospheres and gold nanoparticles modified on the surface; charge transfer can occur between Fe3O4 in the substrate and the dopamine to be tested to form a complex to produce strong plasmon resonance; at the same time, Fe3O4 and gold can generate strong electric fields under Ramanexcitation illumination to enhance the SERS signal of dopamine. The pretreated human serum is mixed with the SERS substrate, the silicon wafer is placed in the mixed solution and separated by a magnet, the obtained silicon wafer contains the magnetic substrate and the dopamine molecule of the complex formed with the iron ions in the substrate, and a SERS detection is performed on the silicon waferto obtain a SERS spectrum of the dopamine molecule. Therefore, the sensitivity of the detection results is high.

The invention discloses a soluble animal protein preparation technology. According to the soluble animal protein preparation technology, animal hair is used as raw materials; firstly, the animal hair is washed cleanly and then soaked in sodium hydroxide solution with the concentration being 1-2% for four hours; secondly, the animal hair and the sodium hydroxide solution are filtered; thirdly, sodium chloride is added to filtrate, wherein the amount of the added sodium chloride is 1-5% of the mass of the filtrate, the filtrate and the sodium chloride are stirred sufficiently to enable the sodium chloride to be completely dissolved, and then still standing of the filtrate and the sodium chloride lasts for 60 minutes; and lastly, the filtrate and the sodium chloride are filtered and dried, and so that soluble animal protein can be obtained.

The invention discloses a novel technology for extracting valine. Animal hair is used as raw materials, the animal hair is washed, cleaned up, immersed in a 1%-2% sodium hydroxide solution for 4 hours and filtered, and filter liquor is used for preparing soluble protein. Acid hydrolysis is carried out on solid substances for 20-60 minutes by using of 5%-20% hydrochloric acid, the solid substances and the hydrochloric acid are stirred ceaselessly, precipitate is filtered out after standing for 60 minutes, and the valine is obtained after the precipitate is dried.

The invention discloses novel process of histidine extraction. Animal hair is used as raw materials, the animal hair is washed cleanly and soaked in 1%-2% of sodium hydroxide solutions for 4 hours, the solution is filtered, and filter liquor is used for preparing soluble protein. Acid hydrolysis is carried out on solid substances for 20-60 minutes by using of 5%-20% hydrochloric acid, the solid substances and the hydrochloric acid are stirred ceaselessly, precipitate is filtered out after standing for 60 minutes, and histidine is obtained after the precipitate is dried.