Liposome drug in-vivo biological analysis method
A technology of bioanalysis and liposome, which is applied in the field of medicine, can solve the problems of not effectively reflecting the bioavailability of drugs, and achieve the effect of precise release degree
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[0036] Example 1: Total multi-soft extraction process (low concentration quantitative range)
[0037] 1. All samples to be tested are completely melted, mix well for 10 to 30 seconds.
[0038] 2. Make 50 μl of the sample to be tested to 1.5 ml of the centrifuge tube, add 50 μl of concentration of 100 ng / ml of IS solution, mix uniform, to obtain a sample to be tested in the internal standard; 50 μl of blank sample (ie 50 μl of blank plasma) to 1.5 In the ml centrifuge tube, 50 μl of the concentration of 100 ng / ml of IS solution was added, mixed uniform, gave a blank internal standard control; 50 μl of whitesample (ie 50 μl of blank plasma) to 1.5 ml of centrifuge tube, add 50 μl of methanol: water (1) : 1), mix well, get a blank control.
[0039]3. 800 μl of chloroform is added to the inner standard, and 800 μl of chloroform is added to the blank internal standard, and the vortex was centrifuged for 3 minutes at 10,000 × g, 4 ° C.
[0040] 4. Remove the underlying organic phase...
Example Embodiment
[0043] Example 2: Total multi-soft extract (high concentration quantitative range)
[0044] 1. All samples to be tested are completely melted, mix well for 10 to 30 seconds.
[0045] 2. Remove 50 μL to be tested to 1.5 ml of the centrifuge tube, add 50 μl of the concentration of 500 ng / ml of IS solution, mix well, to obtain a sample to be tested in the internal standard; 50 μl blank sample (ie 50 μl of blank plasma) to 1.5 In the ml centrifuge tube, 50 μl of concentration of 500 ng / ml is added, mix uniform, gave a blank internal standard control; 50 μl of blank sample (ie 50 μl of blank plasma) to 1.5 ml of centrifugal tube, add 50 μl of methanol: water (1) : 1), mix well, get a blank control.
[0046] 3. 800 μl of chloroform is added to the inner standard, and 800 μl of chloroform is added to the blank internal standard, and the vortex was centrifuged for 3 minutes at 10,000 × g, 4 ° C.
[0047] 4. Remove the underlying organic phase 200 μL to 96-well plates, dry samples at 3...
Example Embodiment
[0050] Example 3: The extraction process of the multirodynamic packed star wrapped in liposomes (low concentration quantitative range)
[0051] 1. All samples to be tested are completely melted, mix well for 10 to 30 seconds.
[0052] 2. Add 100 μl of water to a 50 μl of the sample, mix well, and mixed samples.
[0053] 3. 1 ml of methanol and water activated SPE solid phase extraction plate (Strata C18-E) were added sequentially.
[0054] 4. Sequentially transfer all of the above mixed samples and 150 μl of water to an activated solid phase extraction plate, and the effluent is collected.
[0055] 5. Add 50 μl of the concentration of 100 ng / ml of IS solution to the effluent, mix well, to obtain a sample to be tested in the inner standard. 50 μl of blank sample (i.e., 50 μl of blank plasma) alternatively operated the 50 μl of the sample to be tested in step 5 to step 5, resulting in a blank internal standard control; 50 μl blank sample (ie, 50 μl of blank plasma) is replaced by ...
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