A method for extracting α-arbutin from fermentation broth based on membrane separation technology

Abstract

The invention discloses a method for extracting a-arbutin from fermentation liquor based on membrane separation technology. The method comprises the following steps: (1) microfiltering fermentation liquor; (2) adsorbing and desorbing microfiltrate; (3) concentrating and decolorating desorbed solution; and (4) concentrating and catalyzing the decolorated solution. The ceramic membrane separation technology is applied into the pretreatment of the a-arbutin fermentation liquor, and the method is simple and convenient to operate, high in safety coefficient, low in production cost, high in yield and good in purity and quality compared with the method of flocculating and extracting arbutin in the fermentation liquor with alcohol.

Description

technical field

[0001] The invention relates to the technical field of alpha-arbutin extraction, in particular to a method for extracting alpha-arbutin from fermentation broth based on membrane separation technology. Background technique

[0002] Arbutin belongs to hydroquinone glycoside compounds, the chemical name is 4-hydroxyphenyl-D-glucopyranoside, and natural arbutin is α-arbutin, which exists in bearberry, lingonberry and other plants. A non-irritating, non-allergenic natural whitening active substance. α-Arbutin can significantly inhibit the activity of tyrosinase, reduce the deposition of tyrosinase in the skin, and has the effects of bleaching, anti-pigmentation and freckle removal on the skin. It is an internationally recognized high-efficiency freckle-removing and whitening agent. The demand is huge and the market prospect is broad.

[0003] There are two molecular configurations of arbutin: α-arbutin and β-arbutin. Among them, the effect of α-arbutin on inhib...

Examples

Embodiment 1

[0018] Pass 100L of α-arbutin fermentation broth through a microfiltration membrane with a membrane pore size of 1000nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 8 times, 25L of dialysis water is added. The filtrate passes through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H, and the analysis solution is also passed through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H with 25% ethanol. The analysis solution was concentrated under reduced pressure, and decolorized by D315 macroporous adsorption resin at a flow rate of 2BV / H. The decolorization solution was concentrated twice and crystallized to obtain a total of 408.3g of α-arbutin product. The purity of the α-arbutin product detected by high pressure liquid chromatography is 99.57%.

[0019]

Embodiment 2

[0021] Pass 100L of α-arbutin fermentation broth through a microfiltration membrane with a membrane pore size of 500nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 10 times, 30L of dialysis water is added. The filtrate passes through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H, and the analysis solution is also passed through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H with 25% ethanol. The decolorization solution was concentrated under reduced pressure, and decolorized by D315 macroporous adsorption resin at a flow rate of 2BV / H. The decolorization solution was concentrated twice and crystallized, and a total of 438.7g of α-arbutin product was obtained. The purity of the α-arbutin product detected by high-pressure liquid chromatography is 99.76%.

[0022]

Embodiment 3

[0024] Pass 100L of α-arbutin fermentation broth through a microfiltration membrane with a membrane pore size of 200nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 10 times, 25L of dialysis water is added. The filtrate passes through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H, and the analysis solution is also passed through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H with 25% ethanol. The decolorization solution was concentrated under reduced pressure, and decolorized by D315 macroporous adsorption resin at a flow rate of 2BV / H. The decolorization solution was concentrated twice and crystallized to obtain a total of 416.4g of α-arbutin product. The purity of the α-arbutin product detected by high pressure liquid chromatography is 99.62%.

[0025]

[0026]

technical field

[0001] The invention relates to the technical field of alpha-arbutin extraction, in particular to a method for extracting alpha-arbutin from fermentation broth based on membrane separation technology. Background technique

[0002] Arbutin belongs to hydroquinone glycoside compounds, the chemical name is 4-hydroxyphenyl-D-glucopyranoside, and natural arbutin is α-arbutin, which exists in bearberry, lingonberry and other plants. A non-irritating, non-allergenic natural whitening active substance. α-Arbutin can significantly inhibit the activity of tyrosinase, reduce the deposition of tyrosinase in the skin, and has the effects of bleaching, anti-pigmentation and freckle removal on the skin. It is an internationally recognized high-efficiency freckle-removing and whitening agent. The demand is huge and the market prospect is broad.

[0003] There are two molecular configurations of arbutin: α-arbutin and β-arbutin. Among them, the effect of α-arbutin on inhib...

Examples

Embodiment 1

[0018] Pass 100L of α-arbutin fermentation broth through a microfiltration membrane with a membrane pore size of 1000nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 8 times, 25L of dialysis water is added. The filtrate passes through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H, and the analysis solution is also passed through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H with 25% ethanol. The analysis solution was concentrated under reduced pressure, and decolorized by D315 macroporous adsorption resin at a flow rate of 2BV / H. The decolorization solution was concentrated twice and crystallized to obtain a total of 408.3g of α-arbutin product. The purity of the α-arbutin product detected by high pressure liquid chromatography is 99.57%.

[0019]

Embodiment 2

[0021] Pass 100L of α-arbutin fermentation broth through a microfiltration membrane with a membrane pore size of 500nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 10 times, 30L of dialysis water is added. The filtrate passes through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H, and the analysis solution is also passed through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H with 25% ethanol. The decolorization solution was concentrated under reduced pressure, and decolorized by D315 macroporous adsorption resin at a flow rate of 2BV / H. The decolorization solution was concentrated twice and crystallized, and a total of 438.7g of α-arbutin product was obtained. The purity of the α-arbutin product detected by high-pressure liquid chromatography is 99.76%.

[0022]

Embodiment 3

[0024] Pass 100L of α-arbutin fermentation broth through a microfiltration membrane with a membrane pore size of 200nm, the membrane entry pressure is 0.2MPa, the membrane exit pressure is 0.1MPa, and the operating temperature is 25°C. After the fermentation broth is concentrated 10 times, 25L of dialysis water is added. The filtrate passes through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H, and the analysis solution is also passed through the DA201 macroporous adsorption resin column at a flow rate of 2BV / H with 25% ethanol. The decolorization solution was concentrated under reduced pressure, and decolorized by D315 macroporous adsorption resin at a flow rate of 2BV / H. The decolorization solution was concentrated twice and crystallized to obtain a total of 416.4g of α-arbutin product. The purity of the α-arbutin product detected by high pressure liquid chromatography is 99.62%.

[0025]

[0026]

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