Method for preparing high-purity norbixin

Abstract

The invention discloses a method for preparing high-purity norbixin, which comprises the following steps of: adding annatto seeds into aqueous solution of ethanol or acetone, stirring the solution, and performing solid-liquid separation to obtain bixin extract; enriching and desorbing the extract through adsorbent resin; and concentrating the desorption solution, drying the concentrate to obtain bixin crystal, adding aqueous solution of ethanol and alkali into the bixin crystal to dissolve the crystal, adjusting the pH value, separating out norbixin crystal, and performing solid-liquid separation to obtain the high-purity norbixin. Most impurities with high polarity can be removed by adopting macroporous absorption resin enrichment so as to improve the clarity and quality of the extract. Because a discontinuous gradient desorption method is adopted in the desorption process, a low-concentration ethanol flushing resin column can remove polysaccharide and high-polarity impurities; and the adopted high-concentration ethanol desorption greatly improves the quality of the desorption solution and is favorable for subsequent crystallization. By detecting, the HPLC content is more than 95 percent, and the yield reaches over 80 percent; and the method provides safer technical guarantee for producing medicinal raw materials, has low extraction cost and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a method for preparing high-purity annatto from annatto seeds. Background technique [0002] Annatto (mahogany pigment) is a non-toxic and tasteless natural pigment extracted from the aril of the tropical plant annatto. It has good dyeing properties and can provide three shades of yellow, orange, and orange. Not only It can be widely used as a coloring agent for daily necessities, food, and cosmetics, and it also has certain pharmacological effects. It has been used in Western countries for thousands of years. It is an international functional food coloring. It has passed the review of FAO / WHO and JECFA, and its product marketing is 167 country or region. In our country, it has also been included in the national standard food additive use hygienic standard (GB2760-1996), and many domestic businesses have already been operating and using mahogany pigment. [0...

AI Technical Summary

Problems solved by technology

There are many shortcomings in this method: (1) due to the direct use of alkaline water as the extraction solvent, the extract contains a large amount of sugar, protein and other polar impurities; (2) the extract is not purified by column chromatography, but is directly acidified. Precipitation and crystallization, resulting in low purity of the obtained nor annatto

[0007] The development of annatto pigment in my country is still in its infancy, and the technical level is backward. Due to the limitations of resources, information, technology, etc., although many universities and research institutes have conducted research on annatto pigment, the level and depth of research It is still in its infancy, and the obtained pigment products are basically crude products, which limits its application range

At present, there is no annatto product that has entered the commercialization stage in China, and we completely rely on imports

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