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Method for preparing high-purity annatto

An annatto, high-purity technology, applied in the field of phytochemistry, can solve the problems of low annatto yield, limited application range, backward technical level, etc., achieve safety technical guarantee, improve clarity and quality, and low extraction cost Effect

Inactive Publication Date: 2011-02-16
河北泽华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, the yield of annatto obtained is low because the extract is directly dissolved and crystallized without being purified by column chromatography.
However, the development of annatto pigment in my country is still in its infancy, and the technical level is backward. Due to the limitations of resources, information, technology, etc., although many universities and research institutes have studied annatto pigment, the research level and The depth is still in its infancy, and the obtained pigment products are basically crude products, which limits its application range
At present, there is no annatto product that has entered the commercialization stage in China, and we completely rely on imports

Method used

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  • Method for preparing high-purity annatto
  • Method for preparing high-purity annatto
  • Method for preparing high-purity annatto

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Embodiment 1: the annatto seed is added in the aqueous solution of ethanol or acetone of 2~10 times of (v / w) amount, promptly annatto seed takes weight gram or kilogram as unit of measurement, and the aqueous solution of ethanol or acetone takes volume liter or milliliter For the unit of measurement, the present embodiment gets 1000 grams of annatto seeds (annatto content is 2.5%), adds 4000 ml of ethanol with a volume concentration of 60%, and filters after stirring for 1 hour at 40° C., and the gained annatto extract filtrate is diluted with water to When the ethanol concentration is 50%, pass through the D312, XAD-16 or X-5 macroporous resin adsorption column with a height-to-diameter ratio of 3:1 and a loading capacity of 400ml at a flow rate of 1BV / h, and the adsorption flow rate is controlled at 1.5-2BV / h h, after adsorption, use a volume concentration of 70% ethanol aqueous solution to carry out saturated resin column purification, remove most of the sugar and oth...

Embodiment 2

[0021] Embodiment 2: The difference between this embodiment and Example 1 is to get 10 kilograms of annatto seeds (annatto content is 2.5%), add ethanol 50L of volume concentration 65%, filter after stirring at 45 ℃ for 1.5 hours. Gained annatto extract filtrate adds water and is diluted to ethanol concentration when being 50%, with the flow velocity of 1.5BV / h, be 4: 1 by height-to-diameter ratio, and loading capacity is the CAD-40 or LX700 macroporous resin resin adsorption column of 7500ml to carry out For the capture and enrichment of annatto, the adsorption flow rate is controlled at 1.5-2BV / h. After adsorption, use 70% ethanol aqueous solution to perform saturated resin column purification. After removing most of the sugar and other polar impurities, 95% ethanol aqueous solution (or methanol or acetone) adopt the discontinuous gradient desorption method to carry out resin desorption, the desorption flow rate is controlled at 0.5BV / h, when HPLC detects that annatto flows o...

Embodiment 3

[0022] Embodiment 3: The difference between the present embodiment and Example 1 is to get 100 kilograms of annatto seeds (annatto content is 2.5%), add ethanol 600L of volume concentration 70%, filter after stirring 2 hours under 50 ℃. Gained annatto extract filtrate adds water and dilutes to ethanol concentration when being 50%, with the flow velocity of 2BV / h, be that the adsorption column that HZ818 or HZ801 or D1300 macroporous resin loading capacity is 120L with the flow rate of 2BV / h is 4: 1 carries out annatto extraction. For orange capture and enrichment, the adsorption flow rate is controlled at 1.5-2BV / h. After adsorption, use 70% ethanol aqueous solution to perform saturated resin column purification, remove most of the sugar and other polar impurities, and then use 95% ethanol aqueous solution (or methanol or acetone) adopt the discontinuous gradient desorption method to carry out resin desorption, the desorption flow rate is controlled at 0.5BV / h, when HPLC detect...

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Abstract

The invention discloses a method for preparing high-purity annatto. The method comprises the following steps of: adding annatto seeds to an alcohol or acetone aqueous solution; stirring at 30-60 DEG C and separating solid and liquid to obtain an annatto extracting solution; enriching and desorbing the annatto extracting solution through absorption resin; and decompressing, concentrating, crystallizing and drying an obtained desorbing solution to obtain an annatto crystal. In the invention, because macroporous absorption resin is adopted to enrich the annatto, most high-polarity impurities canbe removed, therefore, the clarity and the quality of the extracting solution are improved; a discontinuous gradient desorbing method is adopted in the desorbing process, and a low-concentration alcohol sluicing resin column can be used for removing most polysaccharides and strong-polarity impurities adsorbed by the resin; and then the high-concentration alcohol is used for desorbing to ensure that the desorbing solution quality is greatly improved, which is beneficial to subsequent crystallization. An obtained product has high purity, wherein shown as detection, the content of HPLC (High Performance Liquid Chromatography) is larger than 95 percent, and the yield reaches higher than 80 percent, thus the invention provides a safer technical guarantee for producing officinal grade raw materials, has low extracting cost and is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a method for preparing high-purity annatto from mahogany seeds. Background technique [0002] Annatto (mahogany pigment) is a non-toxic and tasteless natural pigment extracted from the aril of the tropical plant annatto. It has good dyeing properties and can provide three shades of yellow, orange, and orange. Not only It can be widely used as a coloring agent for daily necessities, food, and cosmetics, and it also has certain pharmacological effects. It has been used in Western countries for thousands of years. It is an international functional food coloring. It has passed the review of FAO / WHO and JECFA, and its product marketing is 167 country or region. In our country, it has also been included in the national standard food additive use hygienic standard (GB2760-1996), and many domestic businesses have already been operating and using mahogany pigment. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07C69/587C07C67/48C07C69/602
Inventor 王辉樊书旗杨贵国
Owner 河北泽华生物科技有限公司
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