Method for extracting genistein from plant nepeta japonica maxim

A technology of genistein and sophora japonicus, applied in separation methods, chemical instruments and methods, solid solvent extraction, etc., can solve problems such as low column efficiency, low chromatographic efficiency, irregular product shape, etc., and achieve solvent usage The effect of reduction, simple operation process, and small amount of solvent used

Inactive Publication Date: 2009-09-30
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bulk polymer obtained by bulk polymerization technology needs to be crushed, ground, and sieved before it can be used. The processing process is long and time-consuming, and the polymer is lost during the grinding and sieving process. low efficiency
The in-situ method is to directly synthesize continuous porous rod-shaped MIPs in the column tube, but the column efficiency is still not high, and there is a problem of high column pressure.
In addition, the limited binding capacity of the synthesized polymer also limits its application in industrial production.

Method used

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  • Method for extracting genistein from plant nepeta japonica maxim
  • Method for extracting genistein from plant nepeta japonica maxim
  • Method for extracting genistein from plant nepeta japonica maxim

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The specific method of this embodiment is carried out according to the following steps:

[0039] (1) Preparation of genistein molecularly imprinted polymer:

[0040] 1) Multi-step swelling polymerization:

[0041] a. First, add 0.012 g of polystyrene microspheres with a particle size of 1 μm to each milliliter of microemulsion A, and react at a constant temperature magnetic stirrer at a speed of 150 rpm for 15 hours at room temperature to obtain the first step swelling liquid;

[0042] Wherein every milliliter of microemulsion A contains 0.002g of sodium dodecyl sulfonate, contains 0.048g of dibutyl phthalate, and the rest is triple distilled water;

[0043] b. Add 2.4 mL of microemulsion B to each milliliter of the first step swelling solution, and react for 2 hours in a constant temperature magnetic stirrer at a speed of 150 rpm at room temperature to obtain the second step swelling solution;

[0044] Wherein every milliliter microemulsion B contains 0.017g of azobi...

Embodiment 2

[0054] The specific method of this embodiment is carried out according to the following steps:

[0055] (1) Preparation of genistein molecularly imprinted polymer:

[0056] 1) Multi-step swelling polymerization:

[0057] a. First, add 0.006 g of polymethacrylic acid microspheres with a particle size of 1 μm to each milliliter of microemulsion A, and react at a constant temperature magnetic stirrer at a speed of 130 rpm for 16 hours at room temperature to obtain the first step swelling liquid;

[0058] Wherein every milliliter of microemulsion A contains 0.003g of sodium dodecyl sulfonate, contains 0.05g of dibutyl phthalate, and the rest is triple distilled water;

[0059] b. Add 1.8mL microemulsion B to each milliliter of the first step swelling solution, and react for 2.5h in a constant temperature magnetic stirrer at a speed of 130rpm at room temperature to obtain the second step swelling solution;

[0060] Wherein every milliliter microemulsion B contains 0.02g of azobis...

Embodiment 3

[0069] The specific method of this embodiment is carried out according to the following steps:

[0070] (1) Preparation of genistein molecularly imprinted polymer:

[0071] 1) Multi-step swelling polymerization:

[0072] a. First, add 0.010 g of fluorine-containing acrylate copolymer microspheres with a particle size of 1 μm to each milliliter of microemulsion A, and react at a constant temperature magnetic stirrer at a speed of 100 rpm for 14 hours at room temperature to obtain the first-step swelling solution ;

[0073] Wherein every milliliter of microemulsion A contains 0.002g of sodium dodecyl sulfonate, contains 0.04g of dibutyl phthalate, and the rest is triple distilled water;

[0074] b. Add 2.0 mL of microemulsion B to each milliliter of the first step swelling solution, and react for 1.5 h in a constant temperature magnetic stirrer at a speed of 120 rpm at room temperature to obtain the second step swelling solution;

[0075] Wherein every milliliter microemulsio...

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Abstract

The invention discloses a multi-step swelling and polymerization-solid-phase extraction method for extracting genistein from plant nepeta japonica maxim, which comprises the following steps: (1) a multi-step swelling and polymerization method is adopted to prepare a genistein molecularly imprinted polymer with uniform size; (2) a genistein molecularly imprinted solid-phase extraction post is prepared; (3) and the genistein molecularly imprinted solid-phase extraction post extracts and separates the genistein in plant nepeta japonica maxim. The novel globular molecule imprinted polymer with uniform grain diameter (3 to 5 mum) has high selectivity to the genistein. The obtained polymer is prepared into the solid-phase extraction post which can be used for extraction, separation and purification of the genistein in the plant nepeta japonica maxim to obtain products with higher purity. The operation process is simple with greatly reduced use amount of solvent, so that the extracting process is safer and more environmental-friendly, and the purpose of impurity removal, purifying and enriching target genistein can be rapidly achieved.

Description

technical field [0001] The invention belongs to the field of pharmaceutical processing and production, and relates to a method for extracting genistein, in particular to a method for extracting genistein from plant Sophora japonicus. Background technique [0002] At present, the commonly used extraction techniques of genistein are as follows: [0003] (1) solvent method and column separation method; [0004] (2) Microwave extraction method; [0005] (3) Molecular imprinting technology. [0006] Among the common extraction methods of genistein mentioned above: the solvent reflux extraction method requires multiple extractions, extractions, decolorization, etc., and column chromatography is also required for separation and purification. This type of technology is cumbersome to operate, the extraction efficiency is low, the cost is high, and the amount of extraction is also limited. [0007] Research on microwave extraction is still in its infancy: parameters in the extract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40B01D11/02B01D15/34B01J20/286B01J20/26B01J20/30
Inventor 傅强肖爱萍郭琦艾力江·阿木提刘荣常春
Owner XI AN JIAOTONG UNIV
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