Extraction and separation method of nicotiflorin in rose flower

A separation method, rose flower technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problem of extraction and separation of pyroside from rose flower, and achieve easy elution and regeneration, wide biological activity , good selectivity of organic matter

Active Publication Date: 2019-07-02
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there is no report about the extraction and isolation of pyroside from rose flowers

Method used

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  • Extraction and separation method of nicotiflorin in rose flower
  • Extraction and separation method of nicotiflorin in rose flower

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for extracting and separating nicotinic glycosides from rose flowers, comprising the steps of:

[0026] 1) Take 2kg of dried rose flowers, crush them, and degrease them with petroleum ether for 3 times at room temperature. Extraction 3 times, each cold soaking extraction time is 7 days, 3 days, 3 days respectively, the extracts are combined and concentrated to obtain 600 g of total ethanol extract; among them, the addition ratio of dried rose flower and petroleum ether is 1kg: 5L , the addition ratio of residue and ethanol is 1kg:5L;

[0027] 2) Dissolve the total ethanol extract (600 g) with an appropriate amount of methanol and mix the sample with a D101 macroporous resin column (600 g). After chromatography on a D101 macroporous resin column, wash with water, 20% ethanol, and 40% ethanol in sequence. Remove and evaporate the solvent to obtain 40% ethanol elution fraction;

[0028] 3) The fraction eluted with 40% ethanol was eluted by atmospheric silica gel...

Embodiment 2

[0034] A method for extracting and separating nicotinic glycosides from rose flowers, comprising the steps of:

[0035] 1) Take 2kg of dried rose flowers, crush them, and degrease them twice with petroleum ether at room temperature. Extracted 2 times, each cold soaking extraction time was 7 days and 3 days respectively, the extracts were combined and concentrated to obtain 490 g of total ethanol extract; among them, the addition ratio of dried rose flower and petroleum ether was 1 kg: 6 L, and the residue The addition ratio with ethanol is 1 kg: 6 L;

[0036]2) Dissolve the total ethanol extract (490 g) with an appropriate amount of methanol and mix the sample with a D101 macroporous resin column (490 g). After chromatography on a D101 macroporous resin column, elute with water, 20% ethanol, and 40% ethanol in sequence , evaporate the solvent to obtain 40% ethanol elution fraction;

[0037] 3) The fraction eluted with 40% ethanol was eluted by atmospheric silica gel column c...

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Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to an extraction and separation method of nicotiflorin in rose flower. The method comprises the following steps: 1) taking dried rose flowers, pulverizing, degreasing the material with petroleum ether at room temperature, then adding ethanol to extract by cold soaking, and extracting an extract to obtain a total ethanol extract; 2) dissolving the total ethanol extract by methanol and mixing the materials, eluting a mixture through water, 20% ethanol, and 40% ethanol in order through a D101 macroporous resin column, and then drying a solvent to obtain a 40% ethanol elution component; 3) performing gradient elution on the 40% ethanol elution component with dichloromethane-methanol by normal pressure silica gel column chromatography, and combining the materials by silica gel thin layer chromatography to obtain 12 components which are marked as component Fr. 4-1 to Fr. 4-12 according to the polarityof the obtained components from small to large; and 4) obtaining the eleventh component Fr. 4-11 by gel column chromatography and high performance liquid chromatography. The method of the invention can quickly and effectively extract and isolate the nicotiflorin contained in the rose flower, and the extraction yield of the nicotiflorin is high.

Description

technical field [0001] The invention belongs to the technical field of plant extraction and separation, and in particular relates to a method for effectively and rapidly extracting and separating pyroside from rose flowers. Background technique [0002] Chinese rose is Rosaceae rose plant rose Rosa chinensis Dried flowers of Jacq. The main components of rose flower are flavonoids such as quercetin and kaempferol; other steroid components such as oleanolic acid, β -Sitosterol, campesterol, cycloeucalyptol, etc., and volatile oil components such as gallic acid, etc. Rose has a variety of pharmacological effects, including inhibiting platelet aggregation, reducing vascular permeability, antifungal and antiviral, antioxidative, and enhancing the body's immunity. [0003] At present, there is no report on the extraction and isolation of pyridoxine from rose flowers. Contents of the invention [0004] The purpose of the present invention is to overcome the defects of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
CPCC07H17/07C07H1/08
Inventor 刘振花康文艺魏金凤马常阳李昌勤呼谧允牛云
Owner HENAN UNIVERSITY
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