Method for extracting genistein from plant nepeta japonica maxim

A technology of genistein and sophora japonicus, applied in separation methods, chemical instruments and methods, solid solvent extraction, etc., can solve the problems of low column efficiency, irregular product shape, low chromatographic efficiency, etc., and achieve the usage of solvent The effect of reduction, small amount of solvent used, and simple operation process

Inactive Publication Date: 2011-08-31
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bulk polymer obtained by bulk polymerization technology needs to be crushed, ground, and sieved before it can be used. The processing process is long and time-consuming, and the polymer is lost during the grinding and sieving process. low efficiency
The in-situ method is to directly synthesize continuous porous rod-shaped MIPs in the column tube, but the column efficiency is still not high, and there is a problem of high column pressure.
In addition, the limited binding capacity of the synthesized polymer also limits its application in industrial production.

Method used

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  • Method for extracting genistein from plant nepeta japonica maxim
  • Method for extracting genistein from plant nepeta japonica maxim
  • Method for extracting genistein from plant nepeta japonica maxim

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The specific method of this embodiment is performed according to the following steps:

[0038] (1) Preparation of genistein molecularly imprinted polymer:

[0039] 1) Multi-step swelling polymerization:

[0040] a. First, add 0.012g of polystyrene microspheres with a particle size of 1 μm to each milliliter of microemulsion A, and react for 15 hours in a thermostatic magnetic stirrer at a rotational speed of 150 rpm at room temperature to obtain the first step swelling liquid;

[0041] Each milliliter of microemulsion A contains 0.002g of sodium dodecyl sulfonate, 0.048g of dibutyl phthalate, and the rest is tri-distilled water;

[0042] b. Then add 2.4mL of microemulsion B to each milliliter of the first step swelling liquid, and at room temperature, react for 2h in a constant temperature magnetic stirrer at a speed of 150 rpm to obtain the second step swelling liquid;

[0043] Among them, each milliliter of microemulsion B contains 0.017g of azobisisobutyronitrile, 0.099g of chl...

Embodiment 2

[0053] The specific method of this embodiment is performed according to the following steps:

[0054] (1) Preparation of genistein molecularly imprinted polymer:

[0055] 1) Multi-step swelling polymerization:

[0056] a. First, add 0.006 g of polymethacrylic acid microspheres with a particle size of 1 μm to each milliliter of microemulsion A, and react for 16 hours in a thermostatic magnetic stirrer at a rotational speed of 130 rpm at room temperature to obtain the first step swelling liquid;

[0057] Each milliliter of microemulsion A contains 0.003g of sodium dodecyl sulfonate, 0.05g of dibutyl phthalate, and the rest is tri-distilled water;

[0058] b. Then add 1.8 mL of microemulsion B to each milliliter of the first step swelling solution, and react for 2.5 hours in a thermostatic magnetic stirrer at 130 rpm at room temperature to obtain the second step swelling solution;

[0059] Each milliliter of microemulsion B contains 0.02g of azobisisobutyronitrile, 0.100g of chloroform and ...

Embodiment 3

[0068] The specific method of this embodiment is performed according to the following steps:

[0069] (1) Preparation of genistein molecularly imprinted polymer:

[0070] 1) Multi-step swelling polymerization:

[0071] a. First, add 0.010g of fluorine-containing acrylate copolymer microspheres with a particle size of 1μm to each milliliter of microemulsion A, and react for 14h in a thermostatic magnetic stirrer at a rotating speed of 100rpm at room temperature to obtain the first step swelling liquid ;

[0072] Each milliliter of microemulsion A contains 0.002g of sodium dodecyl sulfonate, 0.04g of dibutyl phthalate, and the rest is tri-distilled water;

[0073] b. Then add 2.0 mL of microemulsion B to each milliliter of the first step swelling liquid, and at room temperature, react for 1.5 hours at a rotating speed of 120 rpm in a thermostatic magnetic stirrer to obtain the second step swelling liquid;

[0074] Each milliliter of microemulsion B contains 0.015g of azobisisobutyronitril...

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Abstract

The invention discloses a multi-step swelling and polymerization-solid-phase extraction method for extracting genistein from plant nepeta japonica maxim, which comprises the following steps: (1) a multi-step swelling and polymerization method is adopted to prepare a genistein molecularly imprinted polymer with uniform size; (2) a genistein molecularly imprinted solid-phase extraction post is prepared; (3) and the genistein molecularly imprinted solid-phase extraction post extracts and separates the genistein in plant nepeta japonica maxim. The novel globular molecule imprinted polymer with uniform grain diameter (3 to 5 mum) has high selectivity to the genistein. The obtained polymer is prepared into the solid-phase extraction post which can be used for extraction, separation and purification of the genistein in the plant nepeta japonica maxim to obtain products with higher purity. The operation process is simple with greatly reduced use amount of solvent, so that the extracting process is safer and more environmental-friendly, and the purpose of impurity removal, purifying and enriching target genistein can be rapidly achieved.

Description

Technical field [0001] The invention belongs to the field of medical processing and production, and relates to a method for extracting genistein, in particular to a method for extracting genistein from a plant Sophora japonica. Background technique [0002] At present, the commonly used extraction techniques for genistein are as follows: [0003] (1) Solvent method and column separation method; [0004] (2) Microwave extraction method; [0005] (3) Molecular imprinting technology. [0006] Among the common extraction methods of genistein mentioned above: solvent reflux extraction, multiple extractions, extractions, decolorization, etc. are required, and column chromatography is also required for separation and purification. This type of technology is cumbersome to operate, low extraction efficiency, high cost, and the amount of extraction is also limited. [0007] The research on microwave extraction is still in its infancy: the parameters in the extraction process, such as the physica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/40B01D15/34B01J20/30B01D11/02B01J20/26B01J20/286C07D311/36
Inventor 傅强肖爱萍郭琦艾力江·阿木提刘荣常春
Owner XI AN JIAOTONG UNIV
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