Trichoderma and application thereof

A technology of Trichoderma and Trichoderma aculeatus, which is applied in the field of microorganisms, can solve the problems of low sporulation of Trichoderma and restrict large-scale production, and achieves the effects of overcoming low sporulation, easy availability and low price.

Active Publication Date: 2019-01-01
ZHEJIANG FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Microbial liquid fermentation is the basis of low cost and easy industrial production, but Trichoderma produces less spores in liquid culture, and solid-state culture restricts large-scale production, and the living bacteria preparation of Trichoderma strains uses conidia, that is, The number of conidia is directly proportional to the drug effect

Method used

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  • Trichoderma and application thereof
  • Trichoderma and application thereof
  • Trichoderma and application thereof

Examples

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Embodiment Construction

[0023] The following specific examples are further descriptions of the methods and technical solutions provided by the present invention, but should not be construed as limiting the present invention.

[0024] 1. Classification and identification of Trichoderma strains

[0025] Trichoderma aculeatus HTTA-Z0002 strain, in solid MPDA medium (potato juice 200-250ml, peptone 1-2g, glucose 25-30g, NaCl 5-8g, MgCl 2 6H 2 O 0.1-0.3g, KCl 0.1g, agar 18g, water 1000ml), 20°C-23°C, culture for 3-5 days, continuously observe and record changes in colony shape, color, etc., pick mycelium and observe the sporulation structure under an optical microscope . The strain grows vigorously on the MPDA artificial medium, the sporogenous layer and the conidiophores are densely felt-like, green, or white, the conidiophores have septa, and produce lateral branches, which are vertically opposite and have a wider base , the end is thinner, bottle-shaped, conidia monospore, spherical to ovoid, colorl...

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Abstract

The invention discloses trichoderma and application. The trichoderma is Trichoderma asperellum; the strain number is HTTA-Z0002; the preservation number is CGMCC No.15677. The trichoderma can be applied to plant disease prevention and treatment, bacteriostat and pesticide. The strain has stable biological features and pesticide activity; the culture is easy; the culture period is short; the operation method is simple, convenient and feasible; great improvement is realized in aspects of characteristics of bacteriostatic activity, spore generation through liquid culture and the like through being compared with that of wild strains; the defect of small spore production quantity of the trichoderma strain liquid culture is overcome; no pollution is caused on the farm products and environment; wide and strong inhibition effects are realized on various pathogenic bacteria of plants.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a trichoderma and its application in pesticides. Background technique [0002] The pollution of cultivated plants by chemical pesticides is a worldwide hot spot. Along with popularizing day by day of protected cultivation vegetable melon and fruit technology, this problem appears more prominent. The high-temperature and high-humidity environment of the greenhouse meets people's needs for vegetables and fruits in four seasons, and also causes serious diseases regardless of season. Excessive pesticides and hormones cause serious pollution to vegetables and fruits, and their toxicity and potential carcinogenic effects pose a great threat to human health. Biological pesticides meet the requirements of modern society for agricultural production and pesticides due to their outstanding advantages of relatively safe to non-target organisms and good environmental comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 陈杰袁静黄晓慧
Owner ZHEJIANG FORESTRY UNIVERSITY
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