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Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling

A technology for medicinal compositions and young fruits, applied in the field of plant extracts, can solve problems such as skin damage and influence

Inactive Publication Date: 2019-01-04
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to achieve special effects, these products add too many carcinogenic chemicals or environmental hormones, so that consumers use ingredients that will damage the skin and affect health without knowing it

Method used

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  • Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling
  • Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling
  • Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 The preparation method of Cucurbitaceae melon young fruit extract of the present invention

[0024] Watermelon (Citrullus lanatus), known as cold melon in ancient times, is a plant or its fruit of the genus Watermelon in the family Cucurbitaceae. Native to Africa, it is a dicotyledonous flowering plant that is shaped like a vine and has feathery leaves. The fruit it bears is a pseudofruit and belongs to a class botanists call pseudoberries. The outer skin of the fruit is smooth, green or yellow with dark green patterns, and the juicy flesh is red or yellow.

[0025]Jiabao melon (Citrullus lanatus (Thunb.) Matsum et Nakai) is one of the early watermelon varieties in Taiwan, China, formerly known as Jiabao Binggua, a pioneer variety with orange flesh. It is only economically cultivated in Penghu County in Taiwan, China and has a long history; Crisp and special flavor, it is favored by Penghu residents and tourists. Therefore, its output value and farmers' w...

Embodiment 2

[0028] Embodiment 2 The effect of promoting the metabolism of keratinocytes of the young watermelon fruit extract or the young fruit extract of Jiabao melon of the present invention

[0029] The present invention performs assays that promote metabolism in keratinocytes. Human keratinocytes (No. PCS-200-010TM) were purchased from the American Type Culture Collection (ATCC). The cells were cultured in skin basal medium (dermal cell basal medium) (ATCC, U.S., No. PCS-200-030TM), and keratinocyte growth factor (ATCC, U.S., No. PCS-200-040TM) was added, which contained: 2.0 mL of 0.4% bovine pituitary extract (bovine pituitary extract, BPE), 0.5 mL of 0.5 ng / mL recombinant human transforming growth factor α (rh TGF-α), 15.0 mL of 6 mM L-glutamine (L-glutamine ), 0.5mL of 100ng / mL hemisuccinate cortisol (hydrocortisone hemisuccinate), 0.5mL of 5mg / mL recombinant human insulin (rhinsulin), 0.5mL of 1.0mM and 0.5mL of 5mg / mL transferrin (apo-Transferrin ).

[0030] The analysis was...

Embodiment 3

[0032] Example 3 The young watermelon fruit extract or Jiabao melon young fruit extract of the present invention inhibits the secretion of interleukin-8 (IL-8)

[0033] Since it is known that interleukin-8 (interleukin-8, IL-8) has the function of regulating the inflammatory response, therefore, the present invention further detects the effect of young watermelon fruit extract or Jiabao melon young fruit extract on the pro-inflammatory cytokine IL-8. 8 inhibition capacity.

[0034] Using the keratinocytes as in the aforementioned Example 2, the present invention uses the human interleukin 8 (IL-8 / CXCL8) ELISA assay kit (R&D systems company, USA) for analysis. First, in a 24-well culture plate, inoculate 5×10 4 keratinocytes were cultured at 37°C for 24 hours; the medium was carefully removed without disturbing the attached cells, and then 120 μL of 0.25 mg / mL 0.25 mg / mL Jabao melon young fruit extract, Jabao melon Ripe fruit extract or young watermelon fruit extract was used...

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Abstract

The invnetion relates to the field of a plant extract, and particularly to use of a young watermelon fruit and a young chiapao melon fruit in preparing a medical composition for gene controlling. Thecomposition which comprises a young watermelon fruit extract, a young chiapao melon fruit extract or combination thereof can promote keratinocyte metabolism and suppression interleukin-8 secretion through up-regulating expression amounts of nitricoxide synthase3 (NOS3) and von Willebrand factor (vWF) gene and down-regulating the expression amounts of interleukin-8 (IL-8), endothelin1 (EDN1), tissue-type plasminogenactivator (PALT), human coagulation factor (F3), serine protease inhibitor1 (SERPINE1) and intercellular adhesion molecule1 (ICAM1) gene, thereby finally realizing effects of anti-inflammation, anti-acne and reducing blood pressure and blood fat.

Description

technical field [0001] The invention relates to the field of plant extracts, and particularly relates to the use of young watermelon fruit and Jiabao melon young fruit for preparing a pharmaceutical composition for regulating genes. Background technique [0002] Modern people live in a busy green, stressful environment, irregular meals or eat processed food, and face the biggest problem of over-exuberant sebaceous glands in the skin, and men's skin often has more oil than women's skin, especially People over 25 years old with oily skin with large pores, long-term exposure to ultraviolet rays, long-term travel, habit of going to bed late, habit of drinking alcohol and coffee, smoking or being exposed to second-hand smoke and working in air-conditioned environments for a long time, There are often problems such as oily skin, large and clogged pores, and acne. [0003] At the same time, with age, cholesterol will accumulate in blood vessels, causing endothelial cell dysfunctio...

Claims

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Application Information

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IPC IPC(8): A61K36/42A61P29/00A61P17/10A61P9/12A61P3/06
CPCA61K36/42A61P3/06A61P9/12A61P17/10A61P29/00A61K2300/00
Inventor 林咏翔姚采涵
Owner 百岳特生物技术(上海)有限公司
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