Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling

A technology for medicinal compositions and young fruits, applied in the field of plant extracts, can solve problems such as skin damage and influence

Inactive Publication Date: 2019-01-04
百岳特生物技术(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in order to achieve special effects, these products add too many carcinogenic chemicals or environmen

Method used

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  • Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling
  • Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling
  • Use of young watermelon fruit and young chiapao melon fruit in preparing medical composition for gene controlling

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 The preparation method of Cucurbitaceae melon young fruit extract of the present invention

[0024] Watermelon (Citrullus lanatus), known as cold melon in ancient times, is a plant or its fruit of the genus Watermelon in the family Cucurbitaceae. Native to Africa, it is a dicotyledonous flowering plant that is shaped like a vine and has feathery leaves. The fruit it bears is a pseudofruit and belongs to a class botanists call pseudoberries. The outer skin of the fruit is smooth, green or yellow with dark green patterns, and the juicy flesh is red or yellow.

[0025]Jiabao melon (Citrullus lanatus (Thunb.) Matsum et Nakai) is one of the early watermelon varieties in Taiwan, China, formerly known as Jiabao Binggua, a pioneer variety with orange flesh. It is only economically cultivated in Penghu County in Taiwan, China and has a long history; Crisp and special flavor, it is favored by Penghu residents and tourists. Therefore, its output value and farmers' w...

Embodiment 2

[0028] Embodiment 2 The effect of promoting the metabolism of keratinocytes of the young watermelon fruit extract or the young fruit extract of Jiabao melon of the present invention

[0029] The present invention performs assays that promote metabolism in keratinocytes. Human keratinocytes (No. PCS-200-010TM) were purchased from the American Type Culture Collection (ATCC). The cells were cultured in skin basal medium (dermal cell basal medium) (ATCC, U.S., No. PCS-200-030TM), and keratinocyte growth factor (ATCC, U.S., No. PCS-200-040TM) was added, which contained: 2.0 mL of 0.4% bovine pituitary extract (bovine pituitary extract, BPE), 0.5 mL of 0.5 ng / mL recombinant human transforming growth factor α (rh TGF-α), 15.0 mL of 6 mM L-glutamine (L-glutamine ), 0.5mL of 100ng / mL hemisuccinate cortisol (hydrocortisone hemisuccinate), 0.5mL of 5mg / mL recombinant human insulin (rhinsulin), 0.5mL of 1.0mM and 0.5mL of 5mg / mL transferrin (apo-Transferrin ).

[0030] The analysis was...

Embodiment 3

[0032] Example 3 The young watermelon fruit extract or Jiabao melon young fruit extract of the present invention inhibits the secretion of interleukin-8 (IL-8)

[0033] Since it is known that interleukin-8 (interleukin-8, IL-8) has the function of regulating the inflammatory response, therefore, the present invention further detects the effect of young watermelon fruit extract or Jiabao melon young fruit extract on the pro-inflammatory cytokine IL-8. 8 inhibition capacity.

[0034] Using the keratinocytes as in the aforementioned Example 2, the present invention uses the human interleukin 8 (IL-8 / CXCL8) ELISA assay kit (R&D systems company, USA) for analysis. First, in a 24-well culture plate, inoculate 5×10 4 keratinocytes were cultured at 37°C for 24 hours; the medium was carefully removed without disturbing the attached cells, and then 120 μL of 0.25 mg / mL 0.25 mg / mL Jabao melon young fruit extract, Jabao melon Ripe fruit extract or young watermelon fruit extract was used...

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Abstract

The invnetion relates to the field of a plant extract, and particularly to use of a young watermelon fruit and a young chiapao melon fruit in preparing a medical composition for gene controlling. Thecomposition which comprises a young watermelon fruit extract, a young chiapao melon fruit extract or combination thereof can promote keratinocyte metabolism and suppression interleukin-8 secretion through up-regulating expression amounts of nitricoxide synthase3 (NOS3) and von Willebrand factor (vWF) gene and down-regulating the expression amounts of interleukin-8 (IL-8), endothelin1 (EDN1), tissue-type plasminogenactivator (PALT), human coagulation factor (F3), serine protease inhibitor1 (SERPINE1) and intercellular adhesion molecule1 (ICAM1) gene, thereby finally realizing effects of anti-inflammation, anti-acne and reducing blood pressure and blood fat.

Description

technical field [0001] The invention relates to the field of plant extracts, and particularly relates to the use of young watermelon fruit and Jiabao melon young fruit for preparing a pharmaceutical composition for regulating genes. Background technique [0002] Modern people live in a busy green, stressful environment, irregular meals or eat processed food, and face the biggest problem of over-exuberant sebaceous glands in the skin, and men's skin often has more oil than women's skin, especially People over 25 years old with oily skin with large pores, long-term exposure to ultraviolet rays, long-term travel, habit of going to bed late, habit of drinking alcohol and coffee, smoking or being exposed to second-hand smoke and working in air-conditioned environments for a long time, There are often problems such as oily skin, large and clogged pores, and acne. [0003] At the same time, with age, cholesterol will accumulate in blood vessels, causing endothelial cell dysfunctio...

Claims

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Application Information

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IPC IPC(8): A61K36/42A61P29/00A61P17/10A61P9/12A61P3/06
CPCA61K36/42A61P3/06A61P9/12A61P17/10A61P29/00A61K2300/00
Inventor 林咏翔姚采涵
Owner 百岳特生物技术(上海)有限公司
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