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Immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens and application thereof

A porcine circovirus, immunogenicity technology, applied in the direction of virus antigen components, medical preparations containing active ingredients, viruses, etc., to achieve the effect of broad-spectrum protection

Active Publication Date: 2022-06-14
PU LIKE BIO ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005]The systemic disease caused by PCV2 had broken out sporadically as early as 1985. Due to neglect, it led to a large-scale outbreak of the disease in the late 1990s. The new porcine circovirus has similar pathogenic characteristics to PCV2 in terms of PNDS and reproductive disorders, and the protein homology between PCV2 and PCV3 is very low, and the cross-protection of PCV2 vaccine cannot effectively prevent PCV3

Method used

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  • Immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens and application thereof
  • Immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens and application thereof
  • Immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens and application thereof

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Embodiment approach

[0041] As an embodiment of the present invention, in the immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens of the present invention, the pharmaceutically acceptable carrier includes an adjuvant, and the adjuvant Includes white oil, Drake oil, and animal, vegetable, or mineral oils; or aluminum hydroxide, aluminum phosphate, and metal salts; or Montanide TM Gel, carbomer, squalane or squalene, ISA206 adjuvant, saponin, water-in-oil emulsion, oil-in-water emulsion, water-in-oil-in-water emulsion.

[0042] As a preferred embodiment of the present invention, in the immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens of the present invention, the adjuvant is Montanide TM Gel.

[0043] The amount of adjuvants suitable for use in the compositions of the invention is preferably an effective amount. The "effective amount" refers to the amount necessary or sufficient for the adjuvants to...

Embodiment 1

[0065] Example 1 Isolation and identification of porcine circovirus type 3

[0066] 1. Source of disease material

[0067] In a commercial pig farm in China, compared with the historical average, the mortality rate of sows increased by 9.4%, the conception rate decreased by 1.2%, and the number of mummies increased by 8.2%. Clinically, affected sows are anorexic with signs of multifocal papules, macules and superficial dermatitis. Aborted litters contained mummified fetuses of different gestational ages, consistent with symptoms of PCV2-related miscarriages. Although the overall clinical presentation and signs of abortion observed in sows were consistent with reproductive failure caused by porcine circovirus type 2, different tissues in all sows, including kidney, lymph nodes, lungs, skin, and stillbirth, were detected by immunohistochemistry. Both PCR and quantitative PCR were negative for PCV2, PRRSV, PPV, CSFV, and Mycoplasma hyopneumoniae. In order to further investigate ...

Embodiment 2

[0072] Embodiment 2 Construction of pET28a-PCV3-Cap expression vector

[0073] 1. Extraction of PCV3 viral DNA

[0074] The plasmid extraction kit was purchased from Tiangen Biotech; T4DNA Ligase was purchased from BioLab; the pET28a plasmid was purchased from Novagen; the agarose gel recovery kit was purchased from Tianze Biotech, and other reagents were of analytical grade.

[0075] According to the instructions of the virus DNA extraction kit, take 0.2ml porcine circovirus type 3 virus liquid into a sterile 1.5ml centrifuge tube, add 0.4ml VB to the virus liquid, vortex and mix well, and let stand at room temperature for 10 minutes. Add 0.45ml AD buffer to the virus solution and mix vigorously. Put the VB column into a 2ml collection tube, take 0.6ml of the virus solution mixed with the above reagents and add it to the VB column, centrifuge at 14000g for 1 minute, then add the remaining virus solution mixed with the reagents above to the VB column, and centrifuge at 14000g...

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Abstract

The invention relates to an immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens, wherein, the immunogenic composition comprises an immune amount of porcine circovirus type 3 antigen, an immune amount of Porcine circovirus type 2 new gene subtype antigen and a pharmaceutically acceptable carrier. The immunogenic composition has good immunogenicity, and can completely protect the mixed infection of porcine circovirus type 3 and porcine circovirus type 2 in one immunization.

Description

technical field [0001] The invention belongs to the field of veterinary medicine, in particular to an immunogenic composition containing porcine circovirus type 3 and porcine circovirus type 2 antigens and application thereof. Background technique [0002] Porcine circoviruses (Porcine circoviruses, PCV) are single-stranded circular DNA viruses with a genome length of about 1.7 kb, and are one of the smallest animal DNA viruses. Two types of PCV have been identified, porcine circovirus type 1 (PCV1) and porcine circovirus type 2 (PCV2). PCV1 was first identified as a contaminant in PK cell cultures in 1974 and is not pathogenic in pigs. PCV2 was first reported in 1998. It can cause porcine circovirus associated diseases (PCVAD) in pigs under clinical conditions, mainly causing multisystem wasting syndrome, pneumonia, porcine dermatitis and nephrotic syndrome in weaned piglets. And reproductive disorders, mainly manifested as respiratory, urinary, intestinal, lymphatic, car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12A61P31/20
CPCA61K39/12A61K2039/70A61K2039/552A61K2039/5258C12N2750/10034A61P31/20C07K14/01C12N15/62
Inventor 田克恭袁于人李向东殷波肖燕陆洲胡冬学孙进忠莫小兵张许科
Owner PU LIKE BIO ENG
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