Alcohol-soluble beta-glucan extract, preparing method and application thereof and health-care wine

A glucan and alcohol-soluble technology, applied in the field of extraction of plant components, can solve the problems of long production cycle, long extraction time and low product purity, and achieve the effects of short production cycle, good alcohol solubility and simple process

Active Publication Date: 2019-01-04
劲牌持正堂药业有限公司
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AI Technical Summary

Problems solved by technology

The β-glucan extracted by hot water method has high purity, but the extraction amount is low, the extraction time is long, the production cost is high, and it is not suitable for industrial production; the acid extraction method is easy to cause excessive hydrolysis of grain starch, and a large amount of glucose will be mixed into β-glucan In sugar products, the purity of the product is low, which increases the difficulty of the separation process
Alkaline extraction hydrolysis can hydrolyze part of the seed coat cellulose, allowing more β-glucan bound to cellulose to be released into the aqueous solution, increasing the yield of β-glucan, but it takes a long time to extract and the production cycle is long
Moreover, the highland barley β-glucan prepared by the prior art is insoluble in ethanol, which limits the scope of application of β-glucan

Method used

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  • Alcohol-soluble beta-glucan extract, preparing method and application thereof and health-care wine

Examples

Experimental program
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Effect test

preparation example Construction

[0031] The object of the present invention is to provide a kind of preparation method of alcohol-soluble β-glucan extract, it comprises the following steps:

[0032] (1) Acid extraction: take highland barley medicinal materials, add acid solution, and carry out acid extraction at 40°C to 60°C to obtain acid extract. In this embodiment, the method of adding the acid solution is generally: add 15-25 times the amount of water of the highland barley medicinal material to the highland barley medicinal material, and then add citric acid to adjust the pH to 4-6; the acid extraction method is generally: Extract at ~60°C for 2 to 3 times, each time for 1 to 2 hours.

[0033] (2) Enzymolysis: add 0.05% to 0.2% of α-amylase and 0.2% to 0.6% of glucoamylase to the acid extract, and enzymolyze at 40°C to 60°C, and the enzymolysis time is generally 1 to 2 hours. After the enzymatic hydrolysis is completed, the enzymatic deactivation treatment is carried out. The general method of inactivat...

Embodiment 1

[0049] This example provides an alcohol-soluble β-glucan extract, which is extracted by the following preparation method:

[0050] (1) Extraction: Take 20kg of highland barley medicinal material, add acid solution to extract twice, add 300L of water each time, then add citric acid to adjust pH to 4, extract at 40°C for 2 hours, combine the two extracts to obtain acid extract.

[0051] (2) Enzymolysis: Add 0.05% α-amylase and 0.2% glucoamylase to the acid extract, and enzymolyze it at 40°C for 1 hour. Minutes to inactivate the enzyme to obtain the enzymolysis extract.

[0052] (3) Centrifugation: Pass the enzymolysis extract through a decanter centrifuge and a disc centrifuge in turn to collect the supernatant.

[0053] (4) Concentration: Concentrate the clear liquid to 1 time of the quality of the highland barley medicinal material to obtain a concentrated liquid.

[0054] (5) Alcohol precipitation: add 95% ethanol to the concentrated solution, control the ethanol concentrat...

Embodiment 2

[0059] This example provides an alcohol-soluble β-glucan extract, which is extracted by the following preparation method:

[0060] (1) Extraction: Take 20kg of highland barley medicinal material, add acid solution to extract twice, add 500L of water each time, then add citric acid to adjust pH to 6, extract three times at 60°C, each time for 2 hours, combine the two extracts, An acid extract is obtained.

[0061] (2) Enzymolysis: Add 0.2% α-amylase and 0.6% glucoamylase to the acid extract, and enzymolyze at 60°C for 2 hours. Minutes to inactivate the enzyme to obtain the enzymolysis extract.

[0062] (3) Centrifugation: Pass the enzymolysis extract through a decanter centrifuge and a disc centrifuge in turn to collect the supernatant.

[0063] (4) Concentration: Concentrate the centrifuged supernatant to 3 times the quality of the highland barley medicinal material to obtain a concentrated solution.

[0064] (5) Alcohol precipitation: add ethanol to the concentrated soluti...

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Abstract

The invention discloses an alcohol-soluble beta-glucan extract, a preparing method and application thereof and health-care wine, and relates to the technical field of extraction of plant components. The preparing method of the alcohol-soluble beta-glucan extract comprises the steps of taking a medicinal material of hordeum vulgar L.var.nudumHook.f, adding an acid solution, and conducting acid extraction; adding alpha-amylase and glucoamylase into an acid extraction solution for enzymolysis, and then conducting enzyme denaturing to obtain an enzymolysis extraction solution; centrifuging and concentrating the enzymolysis extraction solution to obtain a concentrated solution; adding an ethanol solution into the concentrated solution for alcohol precipitation to obtain an alcohol precipitationsolution; filtering and drying the alcohol precipitation solution. The technology is simple, the operation is convenient, the production period is short, the efficiency is high, and the alcohol-soluble beta-glucan extract is suitable for industrial mass production; the alcohol solublility of the prepared alcohol-soluble beta-glucan extract is good, the alcohol-soluble beta-glucan extract can be dissolved into ethanol of different concentrations and is suitable for producing hordeum vulgar L.var.nudumHook.f beta-glucan products; the alcohol-soluble beta-glucan extract is applied to preparing the health-care wine; the health-care wind contains a large amount of hordeum vulgar L.var.nudumHook.f beta-glucan.

Description

technical field [0001] The invention relates to the technical field of extraction of plant components, and in particular to an alcohol-soluble β-glucan extract, its preparation method, application and health wine. Background technique [0002] Highland barley (Hordeum vulgar L.var.nudumHook.f) belongs to the Gramineae barley crop. Because its seeds have no shell, it is also called naked barley, rice barley, Huai barley and yuan barley. It is mainly distributed in Qinghai and Tibet in my country. , Ganzi Prefecture in Sichuan, Diqing in Yunnan, Gannan in Gansu and other places. Highland barley is the only crop in the alpine region at an altitude of 4200-4500m. It has the characteristics of high protein, high fiber, high vitamin, low fat, low sugar, etc., and is rich in trace elements, β-glucan, etc. [0003] Highland barley contains more β-glucans. Studies have found that β-glucans have the functions of clearing the intestines, lowering blood fat, lowering cholesterol, regula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C12G3/04
CPCC08B37/0003C08B37/0024C12G3/04
Inventor 江丹王鹏陈志元胡力飞
Owner 劲牌持正堂药业有限公司
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