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Bacillus velezensis for preventing and treating rice blast and application of bacillus velezensis

A technology of Bacillus velesi and Bacillus, which is applied in the field of microorganisms to achieve the effects of efficient antibacterial, good antagonism, and broad-spectrum antibacterial

Active Publication Date: 2019-01-04
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and application of bacillus to control rice blast is the need for sustainable development of rice production, and can meet the requirements of the United Nations World Food Organization for the development of food security. Bacillus strains with high production of active substances

Method used

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  • Bacillus velezensis for preventing and treating rice blast and application of bacillus velezensis
  • Bacillus velezensis for preventing and treating rice blast and application of bacillus velezensis
  • Bacillus velezensis for preventing and treating rice blast and application of bacillus velezensis

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Experimental program
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Effect test

Embodiment 1

[0034] The isolation and screening methods of Bacillus veleisi E69 strain are as follows:

[0035] (1) Sample collection: The sample collected is rice variety Ningjing No. 50, and the location is Wutongshu Township, Lingwu City, Ningxia Hui Autonomous Region. It is pulled out together with the root system of the rice, packed in a collection bag, and brought back to the laboratory in an ice box. separate.

[0036] (2) Separation method: select healthy rice plant roots, stems and leaves, weigh 1 g, soak in 1% sodium hypochlorite for 10 minutes, soak in 70% alcohol for 1 minute, add sterile water and wash repeatedly (at least 10 times). Take 100 μL of the last washing solution and spread it on the beef extract peptone agar medium (NA) plate, incubate in a constant temperature incubator at 28°C for 72 hours, and test the surface disinfection effect. The root, stem and leaf segments after surface disinfection are ground into a homogenate in a sterile mortar (adding a small amount ...

Embodiment 2

[0043] Determination of biological activity of Bacillus Velez E69 strain:

[0044] (1) Bacteriostatic assay of Bacillus E69 fermented culture liquid on rice blast fungus: Place blast fungus P131 fungus cake in the center on the tomato oat plate medium, place a sterile Oxford cup at a distance of 3 cm from both sides of the fungus cake, and place in the cup respectively The fermentation broth of Bacillus was added for confrontation culture, and the sterile LB liquid medium was used as a control, and it was cultured in the dark at 28°C in an incubator. Each treatment was repeated in 10 dishes. After 5 days, the diameter of the pathogenic bacteria colony and the zone of inhibition were measured, and the results are shown in Table 1 and figure 2 .

[0045] (2) Determination of the antibacterial effect of Bacillus E69 sterile supernatant on Magnaporthe grisea: Place the Pyricularia grisea P131 bacterial cake in the center on the tomato oat plate medium, place a sterile Oxford cu...

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Abstract

The invention discloses bacillus velezensis (Bacillus velezensis) for preventing and treating rice blast and the application of the bacillus velezensis, and belongs to the technical field of microorganisms. The bacillus velezensis (Bacillus velezensis) E69 disclosed by the invention is preserved in China General Microbiological Culture Collection Center (CGMCC) of China Committee for Culture Collection of Microorganisms, the preservation date is 5 September, 2018, and the preservation number is CGMCC NO.16427. The bacillus velezensis E69 provided by the invention can prevent and treat rice blast of rice, the new field of application of the bacillus velezensis is opened up, and the bacillus velezensis has good preventing effects on the rice blast of the rice, has significant bacterium restraining effects on various plant pathogenic bacteria, is high in preventing and treating efficiency, has good effects of preventing and treating the rice blast of the rice having different varieties inthe south and the north of China, and can reduce the yield loss of rice industry caused by plant diseases which arise suddenly near the collection period of the rice to a larger extent.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus velezensis for controlling rice blast and its application. Background technique [0002] Rice is one of the most important food crops in the world. Rice is the staple food that more than half of the world's population depends on. Rice production accounts for more than a quarter of the world's total grain output, and more than half of China's total grain output. Rice blast (RiceBlast) is caused by the ascomycete Magnaporthe oryzae (T.T.Hebert) Yaegashi&Udagawa (asexual state: Pyricularia oryzae), which is a fungal disease that is sudden and easy to spread. It is widely distributed and occurs in 85 countries around the world. It is one of the most serious rice diseases in my country's northern and southern rice regions. Rice blast can cause damage to rice all year round, and its damage spreads to all parts of rice, including seedling blast, leaf blast, l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 沙月霞任晓利黄泽阳沈瑞清杜玉宁张怡哈学虎叶利国高立原李玉宝马建华
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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