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A strain of Bacillus Velez for controlling rice blast and its application

A technology of Bacillus Velez and Bacillus, applied in the field of microorganisms, to achieve the effects of broad-spectrum anti-bacteria, good antagonism, and good control effect

Active Publication Date: 2020-01-10
INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and application of bacillus to control rice blast is the need for sustainable development of rice production, and can meet the requirements of the United Nations World Food Organization for the development of food security. Bacillus strains with high production of active substances

Method used

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  • A strain of Bacillus Velez for controlling rice blast and its application
  • A strain of Bacillus Velez for controlling rice blast and its application
  • A strain of Bacillus Velez for controlling rice blast and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The isolation and screening methods of Bacillus veleisi E69 strain are as follows:

[0035] (1) Sample collection: The sample collected is rice variety Ningjing No. 50, and the location is Wutongshu Township, Lingwu City, Ningxia Hui Autonomous Region. It is pulled out together with the root system of the rice, packed in a collection bag, and brought back to the laboratory in an ice box. separate.

[0036] (2) Separation method: select healthy rice plant roots, stems and leaves, weigh 1 g, soak in 1% sodium hypochlorite for 10 minutes, soak in 70% alcohol for 1 minute, add sterile water and wash repeatedly (at least 10 times). Take 100 μL of the last washing solution and spread it on the beef extract peptone agar medium (NA) plate, incubate in a constant temperature incubator at 28°C for 72 hours, and test the surface disinfection effect. The root, stem and leaf segments after surface disinfection are ground into a homogenate in a sterile mortar (adding a small amount ...

Embodiment 2

[0043] Determination of biological activity of Bacillus Velez E69 strain:

[0044] (1) Bacteriostatic assay of Bacillus E69 fermented culture liquid on rice blast fungus: Place blast fungus P131 fungus cake in the center on the tomato oat plate medium, place a sterile Oxford cup at a distance of 3 cm from both sides of the fungus cake, and place in the cup respectively The fermentation broth of Bacillus was added for confrontation culture, and the sterile LB liquid medium was used as a control, and it was cultured in the dark at 28°C in an incubator. Each treatment was repeated in 10 dishes. After 5 days, the diameter of the pathogenic bacteria colony and the zone of inhibition were measured, and the results are shown in Table 1 and figure 2 .

[0045] (2) Determination of the antibacterial effect of Bacillus E69 sterile supernatant on Magnaporthe grisea: Place the Pyricularia grisea P131 bacterial cake in the center on the tomato oat plate medium, place a sterile Oxford cu...

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Abstract

The invention discloses a strain of Bacillus velezensis for preventing and treating rice blast and its application, belonging to the technical field of microorganisms. The Bacillus velezensis E69 of the present invention includes: preserved in the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee, the preservation date is September 5, 2018, and the preservation number is CGMCC NO.16427. The Bacillus Velez E69 provided by the present invention can prevent and treat rice blast, open up a new field of related applications of Bacillus Velez, have good control effects on rice blast, and have significant antibacterial effects on various plant pathogenic bacteria, The control efficiency is high, and it has a good control effect on rice blast of different varieties of rice in the south and north of my country, and can greatly reduce the yield loss caused by sudden diseases in the rice industry near the harvesting period.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Bacillus velezensis for controlling rice blast and its application. Background technique [0002] Rice is one of the most important food crops in the world. Rice is the staple food that more than half of the world's population depends on. Rice production accounts for more than a quarter of the world's total grain output, and more than half of China's total grain output. Rice blast (RiceBlast) is caused by the ascomycete Magnaporthe oryzae (T.T.Hebert) Yaegashi&Udagawa (asexual state: Pyricularia oryzae), which is a fungal disease that is sudden and easy to spread. It is widely distributed and occurs in 85 countries around the world. It is one of the most serious rice diseases in my country's northern and southern rice regions. Rice blast can cause damage to rice all year round, and its damage spreads to all parts of rice, including seedling blast, leaf blast, l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 沙月霞任晓利黄泽阳沈瑞清哈学虎叶利国李玉宝
Owner INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL
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