Method for optimizing wheat gene editing efficiency
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SYNGENTA PARTICIATIONS AG
- Publication Date
- 2019-01-04
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
Technical field
[0001] This application relates to the field of gene editing, particularly to gene editing in wheat. This application improves the efficiency of gene editing in wheat. Background technique
[0002] Wheat is the world's main food crop and has an important economic position. From the analysis of chromosomes and genome, wheat has more complex characteristics than crops such as corn and rice. First, wheat is an allohexaploid and has three sets of genomes (AABBDD); second, the wheat genome is about 17Gb. Both maize and rice are diploid (AA), and their genomes are much smaller than those of wheat. The maize genome is 2.5Gb and the rice genome is only 430Mb. At the same time, the wheat genome has more repetitive sequences and transposable originals. These characteristics increase the difficulty of wheat genome improvement to a certain extent [1] .
[0003] Clustered regularly spaced short Palindromic Repeats and its related system (CRISPR / Cas system, Clustered Regular...