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A method for the extraction, inspection and content determination of triterpenoids in sorbifolium sorbifolium

A technology of compounds and triterpenoids, which is applied in the field of extraction, inspection and content determination of biologically active substances in sorbifolium sorbifolium, can solve the cumbersome operation of extraction inspection and content determination, the cumbersome operation steps of the extraction and inspection method, and the easy interference of content measurement results and other problems, to achieve the effect of easy operation, convenient operation and fast deployment speed

Inactive Publication Date: 2021-02-23
LULIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many types of triterpenoids, but the extraction and testing methods generally have disadvantages such as cumbersome operation steps, large amount of organic solvents, not conducive to environmental protection, residual solvents and resin monomers, etc.
[0004] In order to solve the cumbersome extraction, inspection and content determination of triterpenoids in sorbifolium sorbifolium, the process of manual scraping, extraction, recombination, and injection analysis on the mass spectrometer to obtain the mass spectrum is time-consuming and labor-intensive, and the content determination results are easily affected. The problem of interference and inaccuracy, the present invention provides a time-saving, labor-saving, easy-to-operate method that can quickly extract and detect triterpenoids from X. sorbifolium and determine the content

Method used

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  • A method for the extraction, inspection and content determination of triterpenoids in sorbifolium sorbifolium
  • A method for the extraction, inspection and content determination of triterpenoids in sorbifolium sorbifolium
  • A method for the extraction, inspection and content determination of triterpenoids in sorbifolium sorbifolium

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Embodiment

[0046] Step a, the extraction of triterpenoids:

[0047] Pretreatment: Dry the sorbifolia husk, twigs, young leaves, and seed kernels in the shade, crush them, pass through a 100-mesh sieve, add petroleum ether at 60-90°C, and the ratio of solid to liquid is 1:10 (w / v). After soaking for several hours, filter and dry, repeat the above steps 3 times to obtain skimmed powder.

[0048] Ethanol extraction: Weigh 10g each of the degreased fruit shell powder and young leaf defatted powder and add 80% ethanol, weigh 10g each of the defatted twig powder and seed kernel defatted powder and add 70% ethanol, the ratio of solid to liquid is 1:30 (w / v), stirring with a constant temperature magnetic stirrer to form a suspension.

[0049] Microwave ultrasonic extraction: Ultrasonic microwave extraction is divided into three stages of extraction, the time is 6min, 7min, 8min, the temperature is 50°C, 55°C, 60°C, the ultrasonic power is constant, the ultrasonic power is 750W, 850W, 950W, and...

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Abstract

The invention discloses a method for extracting, inspecting and measuring the content of triterpenoids in X. sorbifolium, and relates to the field of extracting, inspecting and measuring the content of biologically active substances in X. sorbifolium. Different parts of X. , add ethanol, ultrasonic microwave extractor to extract in stages, centrifuge filter, concentrate, add methanol to dissolve, filter, and set the volume to 25mL, respectively obtain sample solutions of Xantho sorbifolium fruit shell, twigs, young leaves, and seed kernels for use, and use ultraviolet The spectrophotometer measures the absorbance of the standard at different concentrations, draws a standard curve, and calculates the content and extraction rate of triterpenoids in the sample, uses a mass spectrometer to measure the molecular weight information of the sample, uses infrared to detect its functional group, and uses a polarimeter to measure the optical rotation And specific rotation, evaporation crystallization determination of melting point. The method of the invention saves time and effort, has good separation effect, high purity and accurate results, and is a method for extraction inspection and content determination with simple operation and high efficiency.

Description

technical field [0001] The invention relates to the field of extraction test and content determination of biologically active substances in sorbifolia, in particular to a method for extraction test and content determination of triterpenoids in sorbifolia. Background technique [0002] Xantho sorbifolium, also known as papaya, Wendengge, etc., is a deciduous tree or large shrub of Sapindaceae Xanthocarpus genus. It is produced in northern my country and grows in barren hillsides, valleys and hilly areas at an altitude of 52 to 2260 meters. Its kernels contain 55% to 65% oil, 23% to 26% protein, 8% to 9% total carbohydrates, 2% to 3% total sugar, 5% triterpene saponins, 18 trace elements, etc. Its heartwood, The stems and fruits can be used as medicine. It is a commonly used medicinal material of the Mongolian people. It can dispel rheumatism, reduce swelling and relieve pain, and is mainly used to treat rheumatism. Studies have shown that sorbetia oil has certain curative ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/062
Inventor 杨卫民赵青红秦永其杜京旗张利军
Owner LULIANG UNIV
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