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Reaction container and biochemical analysis method

A reaction vessel, biochemical technology, applied in the direction of biochemical equipment and methods, chemical instruments and methods, biochemical cleaning devices, etc., to achieve the effect of sufficient brightness

Pending Publication Date: 2019-01-04
TOPPAN PRINTING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quantification using real-time PCR (polymerase chain reaction) has been carried out in the past, but it is known that changes in the total amount of nucleic acid used in the test will affect the quantification, so now we have developed a method that does not affect the quantification by the total amount of nucleic acid Digital PCR technology

Method used

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  • Reaction container and biochemical analysis method
  • Reaction container and biochemical analysis method
  • Reaction container and biochemical analysis method

Examples

Experimental program
Comparison scheme
Effect test

experiment example

[0082] The following shows an example of an experiment in which the degree of light transmission of the cover member 4 affects bright-field observation and fluorescence observation. In the experimental examples shown below, the components corresponding to the reaction vessel 1 of the present embodiment are given corresponding symbols.

[0083] In this experimental example, two transparent resin plate-shaped members were used to manufacture the experimental reaction container 10 . The base material 2 is formed of one of the two plate-shaped members. As another plate-shaped member, a partition wall portion 5 is formed with double-sided tape and bonded to the base material 2 as a substitute for the above-mentioned cover member 4 .

[0084] A liquid in which fluorescently labeled beads are dispersed is injected between the two plate-shaped members, and the plurality of recesses 3 are individually sealed with a sealing liquid. The state of the fluorescently-labeled beads accommod...

Embodiment 1

[0098] Example 1 of the present invention is shown below.

[0099] In the laser transmission welding method, a transmissive material that transmits laser light having a specific wavelength and an absorbing material that absorbs the laser light are combined, and pressure is applied from both sides. The boundary surface is irradiated so that the absorber material melts. Accordingly, heat is conducted from the absorbing material to the transmissive material simultaneously with the melting of the absorbing material, and the transmissive material is also melted because the transmissive material is heated beyond the melting temperature of the transmissive material. As a result, a transmissive material having a low absorption rate with respect to the wavelength of irradiated laser light can be welded by the laser transmissive welding method.

[0100] In this example, for a cover member that functions as an absorbing material when a reaction container is manufactured using a laser tr...

Embodiment 2

[0113] Example 2 of the present invention is shown below.

[0114] In this example, for the cover member that functions as an absorbing material when the reaction container is produced by the laser transmission welding method, it is shown that the lid member can be reliably welded to the base material by the laser transmission welding method while using the reaction vessel. A specific example of fluorescence observation using the fluorescence transmitted through the cover member in the biochemical reaction of the container.

[0115] The material of the substrate in this embodiment is cycloolefin polymer (COP) (with a thickness of 0.3mm˜1mm). As the material of the cover member in this example, a carbon-added COP material (black: transmittance 0.01%, 0.1%, 0.8%, 6%, 24%, 47%) was used to produce the cover member. The carbon-added (carbon-containing) COP material can be selected from commercially available carbon materials for resin (plastic) coloring, and can be mixed when mak...

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Abstract

This reaction container is provided with: a transparent base material having a plurality of recessed portions forming openings in a first surface of the transparent base material; and an infrared rayabsorbent cover member which is welded to the base material at the outside of a region including the plurality of recessed portions in the first surface such that a gap between the cover member and the first surface is formed within the region, wherein light having a wavelength within at least a partial range of a visible light wavelength range can pass through the cover member.

Description

technical field [0001] The invention relates to a reaction vessel and a biochemical analysis method. [0002] this application claims priority based on Japanese Patent Application No. 2016-089362 for which it applied in Japan on April 27, 2016, The content is used here. Background technique [0003] It has been known to diagnose diseases and constitutions by analyzing biomolecules. [0004] For example, constitution diagnosis by analysis of Single Nucleotide Polymorphism (SNP) recorded in DNA, judgment of administration of anticancer drugs by analysis of somatic mutations, and analysis of viral proteins are known. Infectious disease response, etc. [0005] For example, for cancer therapeutic drugs, it is taught that by quantifying the amplification amount (copy number) of EGFR (epidermal growth factor receptor) gene mutation before and after administration of EGFR-TKI (tyrosine kinase inhibitor), it is possible to become an indicator of treatment efficacy. Quantification...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00C12M1/00C12N15/09C12Q1/68G01N21/64G01N33/50G01N33/68G01N37/00
CPCC12M1/00C12Q1/68C12N15/09G01N37/00G01N33/50G01N33/68B01L3/50853B01L3/50851B01L2300/0819B01L2300/0645B01L2300/0654B01L2300/04B01L2300/12B01L2300/168G01N2201/0686G01N2021/6482G01N21/0303G01N2021/0389G01N21/645G01N2021/0325G01N21/6452G01N21/00G01N21/64B01L3/502B01L2200/0689C12M1/3446C12N15/1013C12Q1/6844G01N21/6428
Inventor 松川昌洋牧野洋一星野昭裕
Owner TOPPAN PRINTING CO LTD
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