Prunus cerasifera pollen collecting and preserving method

Abstract

The invention discloses a Prunus cerasifera pollen collecting and preserving method. The method comprises steps: (1) mature and unbroken anther with filaments in the development stage of Prunus cerasifera is collected, and the anther is laid on paper with a drier underneath and is then sealed and dried; (2) when the filaments are dried, the anther is put in a covered plastic tube with a round bottom, the plastic tube is shaken, pollen is made to be dispersed from the anther until the anther stops dispersing the pollen, the pollen is attached to the wall of the plastic tube, the pollen is poured out, the wall of the plastic tube is flicked to enable impurities in the pollen to drop and be removed, and the plastic tube is sealed; and (3) the plastic tube is placed in a larger tube, liquid isinjected to the larger tube, and freezing and preserving are carried out. According to the Prunus cerasifera pollen collecting and preserving method disclosed in the invention, the Prunus cerasiferapollen can be collected with less time, the pollen loss is less, the pollen activity is high, the pollen extracted from the frozen state is not easy to lose activity due to a double-layer tube design,the design is ingenious, the operation is simple and convenient, and large-scale popularization and application are facilitated.

Description

technical field [0001] The invention relates to the technical field of hybrid breeding, in particular to the technical field of pollen collection and storage, in particular to a method for collecting and storing red leaf plum pollen. Background technique [0002] Hybrid breeding is the main way to cultivate new varieties of plants. It has been mastered and utilized by scientific and technological workers engaged in plant breeding for a long time. In the 1960s and 1970s in my country, many colleges and universities and scientific research institutes carried out hybrid breeding. Related research. [0003] Failure to meet the flowering period usually causes difficulties in hybrid breeding. Some trees and garden plants can be solved by prolonging the flowering period, and some have to store pollen. Pollen storage is of great significance to hybrid breeding of some garden plants. Through pollen storage, it can make some late-flowering garden plants and early-flowering garden plan...

AI Technical Summary

Problems solved by technology

[0006] However, the amount of pollen in Prunus chinensis is small, and the collection of inflorescences is time-consuming and labor-intensive due to inconsistent pollen dispersal time; direct flower picking is difficult to disperse pollen after the anthers are dried, and the above-mentioned common pollen collection and storage methods easily lead to excessive pollen loss , especially unfavorable for the pollen collection and preservation of red-leaf plums with a small amount of pollen, and it takes a long time, and the storage method is not conducive to maintaining the activity of pollen