191results about How to "Combined realization" patented technology

The invention provides a nucleic acid extraction lysate for cast-off cells in human feces. The nucleic acid extraction lysate comprises 1-8 mol/L guanidinium, a 1-20 mM metal ion chelating agent and a100 mM Tris-HCl buffer solution with the pH of 7.5-8.0, wherein guanidinium is selected from at least one of guanidine hydrochloride, guanidinium isothiocyanate and guanidine thiocyanate; the metal ion chelating agent is selected from at least one edetic acid and water soluble salt of edetic acid. Preferably, the nucleic acid extraction lysate further comprises a surfactant with the volume of 1-20%, the surfactant is a non-ionic detergent, and the non-ionic detergent is selected from at least one of Tween-20, Tween-80, Brij-35, NP40 and Triton-100. The invention further provides a related preparation method and application. The nucleic acid extraction lysate can quickly and efficiently realize cell lysis, fully releases nucleic acid, meets the consumption demand for follow-up nucleic aciddetection and is suitable for large-scale popularization.

The invention provides a magnetic field generation device based on a magnetic metamaterial and a wireless electric energy transmission system. The magnetic field generation device mainly consists of an energy emitting end and a magnetic metamaterial assembly, wherein the magnetic metamaterial assembly is loaded onto the energy emitting end and has the sub-wavelength characteristic; the magnetic metamaterial assembly comprises a plurality of magnetic metamaterial units. The magnetic field generation device has the advantages that the magnetic metamaterial unit of which the frequency tuning range is controlled within the kilohertz frequency band is excited by an emitting coil, so that each magnetic metamaterial unit can be coupled by a magnetic field to form a special channel band; the different magnetic field distribution can be correspondingly excited at different frequency points within the channel band, so that a magnetic field which is relatively uniformly distributed in a large area can be obtained through regulating and controlling the frequency, thereby realizing the magnetic field generation device capable of performing wireless electric energy transmission with multiple targets, and the wireless electric energy transmission containing the magnetic field generation device.

The invention provides a pipe machining platform for building construction and a pipe bending equipment of the platform. The machining platform comprises a workbench and the pipe bending equipment fixedly connected onto the workbench; the pipe bending equipment comprises a casing as well as a pipe fixing mechanism, a pipe bending mechanism and an angle adjusting mechanism which are arranged on thecasing; the pipe fixing mechanism is used for clamping and fixing pipes to the equipment for subsequent pipe bending operation; and the pipe bending mechanism is used for performing pipe bending machining on the fixed pipes. According to the machining platform, the pipe bending angle can be controlled by the aid of the pipe bending equipment without intentional observation, so that the working efficiency can be improved, and the mis-operation rate can be reduced.

The invention provides a cultivation method for organic rice. Green manure, rape seed cakes and a biological bacterial fertilizer are used as production fertilizer sources. Preferably, weeds and insects are killed manually. Rational close planting is adopted. The method further comprises a step of improving soil fertility, wherein green manure is applied to soil. The method further comprises a step of cultivating rice seedlings, specially comprises the steps of: selecting and treating rice seeds; and then sowing the rice seeds in a proper period. The method further comprises a step of applying a fertilizer, wherein rape seed cakes and the biological bacterial fertilizer are applied. The method further comprises a step of field management. The invention further provides related organic rice. The cultivation method for organic rice provided by the invention can better use the biological bacterial fertilizer and green manure. Soil is planted while being cultivated, so that the activity of soil is improved, the fertility of soil is improved, and the residual amount of heavy metal ions and pesticides is reduced. The cultivation method is skillful in design, simple to use, safe and environmental-friendly and low in production cost. The obtained organic rice is low in pesticide residual amount, does not harm human health, and is high in yield and good in quality, the economical benefit is increased, and the organic rice is suitable for being popularized and applied on a large scale.

The invention discloses a pulse load genset, comprising a prime motor, a generator, a flywheel body, a common base and multiple flywheel pieces with different inertias, wherein the prime motor, the generator and the flywheel body are integrated with bearings into a whole in an aligned positioning manner through an elastic coupling, a connecting flange and a transmission shaft to form the pulse load genset which is arranged on the common base; and the flywheel pieces of each inertia can be arranged on and integrated with the flywheel body into a whole to form flywheels with different inertias. The invention can realize combined variations of multiple schemes for flywheel inertias on the premise of ensuring that each equipment and structure of the genset do not change basically, and has low cost, convenient alternation and good reliability.

The invention discloses a method for cutting, intensive trenching and returning-to-field of crop straw or green manure crop. The method includes the steps of (1), digging a back-filling trench in thefield and placing trenched soil at the edge of the back-filling trench; (2), cutting crop straw or green manure crop in the field; (3), conveying the cut crop straw or green manure crop into the back-filling trench; and (4), filling the trenched soil back to the back-filling trench while compacting the soil. The invention further provides associated equipment for implementing the method. The method is unique and novel, can realize improvement of soil fertility through returning-to-field of crop straw or green manure crop, can also overcome troubles in tillage, fertilizer applying, seeding, germinating and the like due to returning-to-field of crop straw or green manure crop by turning or covering; the associated equipment is of skillful design, easy and convenient to operate, low in cost and suitable for promotion and application in large scale.

The invention provides a special indoor construction plateand an installation methodthereof. The construction plate comprises a hollow outer frame plate, a hollow inner frame plate, a damping mechanism and a sound adsorption sponge layer, wherein the hollow outer frame plate and the hollow inner frame plate are connected in a sleeving mode; the damping mechanism is arranged in the outer frame plate and the inner frame plate, and the sound adsorption sponge layer is arranged outside the construction plate; the problem of insufficient performance of an existing construction plate protection wallspace is solved, and a construction plate with the function of sound absorption and damping simultaneously is achieved. In addition, concave and convex matched mechanisms are arranged on the oppositelateral sides of the circumferential side of the construction plate, so that an initial positioning structure is formed; fixing plates are arranged on two sides of the diagonal positions, screw holesare formed in the other two sides of the diagonal positions, and therefore a fixed installation structure is formed; the problem that an existing construction plate is not easy to install repeatedlyis solved; and the construction plate and the installation method with high installation convenience are realized.

The invention discloses a gymnadenia conopsea extraction method, which comprises the following steps of:(1) soaking roots of the gymnadenia conopsea in water, fully soaking the roots, until the rootsof the gymnadenia conopsea are sampled and observed without white cores;(2) carrying out wet superfine grinding on the roots of the gymnadenia conopsea to obtain dispersion slurry;(3) adding additional water into the dispersion slurry to obtain diluted dispersion liquid, heating to make the temperature rise, then adding neutral protease, and carrying out circulation homogenization extraction through a homogenizing pump to obtain extract liquid;(4) carrying out heat preservation and enzyme inactivation;(5) carrying out crude filtration to obtain crude filtrate; and (6) carrying out fine filtration to obtain refined filtrate. By means of the method, the related extract of gymnadenia conopsea is also provided. The method for extracting the gymnadenia conopsea is unique and novel, the extracted gymnadenia conopsea extract has safety and unique application value in the aspects of skin care, wound repair, wrinkle resistance, sun protection, oxidation resistance, allergy resistance, skin moisture retention and the like, has good application prospect in the field of cosmetics, and is ingenious in process design, simple and convenient to operate and low in cost.

The invention discloses a method for measuring a rice amylose content through singles grain of rice. The method comprises the following steps that: a plurality of rice varieties with different amylose contents are selected as reference samples; the amylose contents are measured separately; the rice of the reference samples and the rice of to-be-tested samples are dried until the weight of the rice of the reference samples and the rice of the to-be-tested samples is constant, a single grain of rice is selected from the reference samples and the to-be-tested samples respectively, and husked and weighed, and then, each husked grain of rice is added into a NaOH solution, and the NaOH solution with the husked grain of rice is vibrated; water is added into the NaOH solution until the total volume of the NaOH solution and the water reaches first volume; centrifugation is performed, and a supernate is obtained; and a glacial acetic acid solution and an iodine-potassium iodide solution are added into a centrifuge tube where the supernate is arranged, the volume of the glacial acetic acid solution and the volume of the iodine-potassium iodide solution are equal to the volume of the supernate; water is added into the centrifuge tube until the total volume of the supernate, the glacial acetic acid solution, the iodine-potassium iodide solution and the water reaches second volume, an obtained mixture stands still, a color developing solution is obtained; absorbance values is measured, with the amylose contents of the reference samples adopted as vertical coordinates, and the absorbance values of a grain of rice of 0.025g converted from the above absorbance values adopted as abscissas, a standard curve is drawn, or a regression equation is obtained; and the amylose contents of the to-be-tested samples are calculated according to the absorbance values of the grain of rice of 0.025g converted from the absorbance values of the to-be-detected samples. The method is unique and novel. With the method adopted, the rice amylase contents can be measured by single grains of rice, and measurement is accurate and reliable.

The invention provides a wireless power transfer system and transfer method. The wireless power transfer system mainly consists of a signal source, an exciting coil, a pair of resonant coils with thesame resonant frequency, a receiving coil and a load; the pair of resonant coils, formed by multiple winding litz wires and loading capacitors on the side surface of an organic glass plate, are used to adjust and control the coil resonant frequency; and by virtue of single-mode point physical properties of a non-Hermitian system, the work frequency can be at the resonance frequency of the resonantcoils, for controlling the distance between the receiving coil and the adjacent resonant coil to adjust the energy transmission rate coupled out of the system, so that by changing the coupling distance of the pair of resonant coils, it is ensured that the system is in the single mode point, and the disadvantages in the prior art are overcome; and therefore, efficient wireless power transfer and single-frequency efficient wireless power transfer at the kilohertz frequency band are realized in a condition of changed coupled distances by virtue of the single single-mode point fixed frequency andthe physical properties with the minimum loss.

The invention provides a support system with an underground horizontal support and a construction method thereof. A horizontal propelling device and end pipes with disc cutters at the front ends are previously arranged on pillars, the pillars are lowered to the same height positions at the bottoms of corresponding foundation pits via preset pile holes, and the end pipes are driven to move towardspillars at the other side by the horizontal propelling device, forming a space capable of being used for installing the pipe joints of steel pipes section by section, so that horizontal support is formed in soil after the installation of the steel pipes without digging the foundation pits, and the technical problem that effective control is difficult to carry out on the deformation of the foundation pits if a horizontal support construction mode is set after the foundation pits are dug is solved. The purposes of greatly improving the rigidity of the foundation pit support system, effectively reducing the bottom deformation of the foundation pits and the shift of the foundation pit support system during the digging of the foundation pits and effectively improving the safety performance of the foundation pit support system and the like are achieved.

The invention provides an assembling type steel structure residence system and a node installation method thereof. Steel column components with wide-flat shape rectangular steel pipes as main body constitution is arranged in corner positions and the middle of the residence, wherein cross section width of the steel pipes is larger than or equal to wall thickness, and cross section length of the steel pipes is longer, and a narrow column structure is formed; and the purpose of hiding steel columns in walls is achieved so that lateral support is not needed to be arranged additionally in the longitudinal direction in the residence. In addition, corbels arranged along the plane long edge extension direction of the residence are arranged on the steel column components in a matched manner, and nodes are moved outside from the steel column components by the corbels. Moreover, connection installation of steel beam components and the steel column components or connection installation of the steel beam components and the corbels is conducted through a bolt assembly, and the purpose of minimization of onsite welding work is achieved.

The invention provides a roller hot press molding manufacturing method of a shoe outsole. TPU mebrane prepared through a tape casting technology is adopted, the TPU mebrane is subjected to hot-pressing when transported through the roller of which the surface is provided with a feature die, so that a three-dimensional mebrane shell-shaped structure is formed on the TPU mebrane,a TPU mebrane outsoleis formed after die cutting, the TPU mebrane outsole can be put in a die and processed together withmidsole materials, and the condition that an integrated and molded shoe sole complex can be prepared by directly bonding and moulding after once heating in the die is achieved;the technical problem of manufacture procedures of rubber outsoles in the prior art is effectively solved, the shoe outsolemanufacturing method with the advantages ofbeing simple in manufacturing process, capable of completely recycling rough selvedge and wastes, high in manufacturing speed and the like is provided, theadvantages of shortening the manufacturing procedures of shoe soles, reducing energy consumption, reducing labor costs,satisfying smart production and the like are achieved, simultaneously the characteristics of TPU materials are used, and the shoe solesmanufactured by the roller hot press molding manufacturing method of the shoe outsolehave more excellentwear-resisting, anti-slip, soft and comfort properties compared with the rubber shoe soles in the prior art.