The invention discloses a method for measuring a rice
amylose content through singles grain of rice. The method comprises the following steps that: a plurality of rice varieties with different
amylose contents are selected as reference samples; the
amylose contents are measured separately; the rice of the reference samples and the rice of to-be-tested samples are dried until the weight of the rice of the reference samples and the rice of the to-be-tested samples is constant, a single grain of rice is selected from the reference samples and the to-be-tested samples respectively, and husked and weighed, and then, each husked grain of rice is added into a NaOH solution, and the NaOH solution with the husked grain of rice is vibrated; water is added into the NaOH solution until the total volume of the NaOH solution and the water reaches first volume;
centrifugation is performed, and a supernate is obtained; and a glacial
acetic acid solution and an
iodine-
potassium iodide solution are added into a
centrifuge tube where the supernate is arranged, the volume of the glacial
acetic acid solution and the volume of the
iodine-
potassium iodide solution are equal to the volume of the supernate; water is added into the
centrifuge tube until the total volume of the supernate, the glacial
acetic acid solution, the
iodine-
potassium iodide solution and the water reaches second volume, an obtained mixture stands still, a color developing solution is obtained;
absorbance values is measured, with the amylose contents of the reference samples adopted as vertical coordinates, and the
absorbance values of a grain of rice of 0.025g converted from the above
absorbance values adopted as abscissas, a
standard curve is drawn, or a regression equation is obtained; and the amylose contents of the to-be-tested samples are calculated according to the absorbance values of the grain of rice of 0.025g converted from the absorbance values of the to-be-detected samples. The method is unique and novel. With the method adopted, the rice
amylase contents can be measured by single grains of rice, and measurement is accurate and reliable.