Medium and method for cultivating inonotus hispidus through utilizing cottonseed hulls

A technology of cottonseed hulls and P. pilofus is applied in the field of medium for cultivating P. pilosicus, which can solve the problems of inability to use P. pilosicus in industrialized cultivation, unfavorable for industrialization, automated production, resource and environmental consumption, and the like. , to achieve the effect of facilitating factory cultivation, shortening the cultivation period and expanding production benefits

Inactive Publication Date: 2019-01-11
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional cultivation of Porpus pilosa is mostly inoculated with solid strains and cultivated with soil, and its growth cycle can reach one year, which is not conducive to industrialization and automatic production; the choice of cultivation medium is mostly segmented wood, which is troublesome and expensive to produce
Today, when the contradiction between bacteria and forests is becoming more and more prominent, the huge consumption of resources and environment caused by the soil-covered cultivation of segment trees makes it no longer suitable for the industrial cultivation of Poria pilosa.
Long cultivation period and high input cost have become the key issues restricting the improvement of economic benefits of the industrial cultivation of P. gracilis

Method used

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  • Medium and method for cultivating inonotus hispidus through utilizing cottonseed hulls

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Effect test

Embodiment 1

[0020] The method of using 100% cottonseed hulls and nutrient solution to cultivate Ciliomycetes pilosula, the material-liquid ratio of cottonseed hull to nutrient solution is 1:1.4, and the specific nutrient solution components are calculated by mass percentage: glucose 2%, yeast extract powder 2% , The balance is water, the sum of each component is 100%, and the pH is natural.

[0021] The specific implementation steps are as follows:

[0022] (1) Preparation of nutrient solution: Weigh glucose and yeast extract powder according to the above nutrient solution formula and dissolve it in water to prepare a nutrient solution.

[0023] (2) Preparation of culture medium: Weigh a certain amount of cottonseed hulls, then add the above-prepared nutrient solution according to the material-to-liquid ratio of 1:1.4, mix well and wait for the nutrient solution to be fully absorbed, and then dispense it into the cultivation bottle. Sterilize in an autoclave at 121°C for 20 minutes and take it ...

Embodiment 2

[0030] The method of using 80% cotton seed hulls and 20% sawdust with nutrient solution to cultivate Ciliomyces pilaris, the ratio of the culture material to the nutrient solution is 1:1.6, and the specific nutrient solution components are calculated by mass percentage as: glucose 1.8%, Yeast extract powder is 0.15%, the balance is water, the sum of each component is 100%, and the pH is natural.

[0031] The specific implementation steps are as follows:

[0032] (1) Preparation of nutrient solution: Weigh glucose and yeast extract powder according to the above nutrient solution formula and dissolve it in water to prepare a nutrient solution.

[0033] (2) Preparation of culture medium: Weigh a certain amount of cottonseed hulls and sawdust in proportion, then add the above-prepared nutrient solution according to the material-to-liquid ratio of 1:1.4, mix evenly, and then dispense into the cultivation bottle after the nutrient solution is fully absorbed , Sterilize the cultivation bot...

Embodiment 3

[0040] Using 80% cottonseed husks and 20% corn cobs with nutrient solution to cultivate Ciliomyces hirsuta, the ratio of the culture medium to the nutrient solution is 1:1.5, and the specific nutrient solution components are calculated by mass percentage as: glucose 2.2% , Yeast extract powder is 0.25%, the balance is water, the sum of each component is 100%, and the pH is natural.

[0041] The specific implementation steps are as follows:

[0042] (1) Preparation of nutrient solution: Weigh glucose and yeast extract powder according to the above nutrient solution formula and dissolve it in water to prepare a nutrient solution.

[0043] (2) Preparation of culture medium: Weigh a certain amount of cottonseed husks and corn cobs in proportion, then add the above-prepared nutrient solution according to the material-to-liquid ratio of 1:1.4, mix well and wait for the nutrient solution to be fully absorbed, and then dispense to the cultivation bottle In the middle, sterilize the cultivat...

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Abstract

The invention provides a culture medium and method for cultivating inonotus hispidus through utilizing cottonseed hulls. The culture medium is characterized in that the cottonseed hulls are taken as amain cultivation substrate, 0-20% of cultivation auxiliary materials (corn core, sawdust, wheat or rice) are added according to a certain ratio, the nutrient solution with the material-liquid mass ratio of 1:1.4-1:1.6 is then mixed, after sterilization, the medium is obtained. The nutrient solution comprises, by mass percentage, 1.8-2.2% glucose, 0.15-0.25% yeast extract powder, the balance is water, the sum of the components is 100%, the pH is natural, after uniformly mixing each 40 parts of cultivation materials, 55-65 parts of the nutrient solution are added. The medium is advantaged in that properties of low cost and high biological conversion rate are achieved, and the medium is simple to prepare, is convenient to operate and is easy for factory realization.

Description

Technical field [0001] The invention relates to the field of edible fungus cultivation, in particular to a culture medium and method for cultivating Ciliomycetes pilosula by using cotton seed hulls. Background technique [0002] Inonotus hispidus belongs to Basidiomycota, Agaricomycetes, Hvmenochaetales, Hymenochaeetaceae, Inonotus. Ciliomyces lasicum is a facultative saprophytic bacteria, mainly parasitic. It is used to treat gastric diseases such as indigestion in Northeast China, and it is widely used in the treatment of various difficult diseases in southern Xinjiang. The decoction of this bacteria is mainly used to treat various cancers. , Diabetes, gout, arthritis, etc. It is reported that the bacteria has anti-cancer effects, and the inhibition rates of mouse sarcoma S180 and Ehrlich's carcinoma are 80% and 70%, respectively. The bacteria also has medicinal functions such as hemostasis and dispelling wind. The medicinal value of Ciliomyces lasicum has attracted more and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/50A01G18/00A01G18/20
Inventor 傅俊生任亚倩刘鑫吴小平郑明锋
Owner FUJIAN AGRI & FORESTRY UNIV
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