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Device and method for preparation of serum rich in alpha 2 macroglobulin by improved ultrafiltration centrifugation process

A macroglobulin and centrifugation technology, which is applied in the field of devices for preparing α2 macroglobulin-rich serum by an improved ultrafiltration centrifugation method, can solve the problems of increasing the probability of contamination of blood products, reducing protein activity, cumbersome extraction steps, and the like. High recovery rate, good reproducibility, easy to detect the desired effect

Pending Publication Date: 2019-01-15
王小虎 +3
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Problems solved by technology

[0026] In order to solve the problems that the existing α2 macroglobulin purification method has cumbersome extraction steps, the protein activity gradually decreases during the extraction process, and the contamination probability of blood products is also increased in the complex extraction process, an improved method is provided. Device and method for preparing serum rich in α2 macroglobulin by ultrafiltration and centrifugation

Method used

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  • Device and method for preparation of serum rich in alpha 2 macroglobulin by improved ultrafiltration centrifugation process
  • Device and method for preparation of serum rich in alpha 2 macroglobulin by improved ultrafiltration centrifugation process
  • Device and method for preparation of serum rich in alpha 2 macroglobulin by improved ultrafiltration centrifugation process

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Embodiment

[0058] Example: Clinically, patients who meet the treatment conditions will collect blood through the cubital vein, then extract 50ml of venous blood through the first suction device 2, inject it into a 50ml ordinary centrifuge tube 5, and centrifuge for 20min under a centrifugal force of 2000g;

[0059] Through the second suction device 3, the supernatant (serum) 7 is collected and injected into the ultrafiltration centrifuge tube 8, and then at a low temperature of 4 degrees, with a centrifugal force of 5000g, and a centrifugation time of 90min; -M serum, and injected into the patient's own joint cavity.

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Abstract

The invention relates to the technical field of blood products, in particular to a device and method for preparation of serum rich in alpha 2 macroglobulin by an improved ultrafiltration centrifugation process, and solves the problems of tedious extraction steps, progressively decreased protein activity in the extraction process and increased blood product contamination probability caused by the complicated extraction process in the existing alpha 2 macroglobulin purification method. The device consists of a centrifuge pipe and suction equipment, the centrifuge pipe consists of a common centrifuge tube and an ultrafiltration centrifuge tube, two ends of a first suction apparatus are respectively connected to target blood and the common centrifuge tube, two ends of a second suction apparatus are respectively connected to the common centrifuge tube's part accommodating supernatant and the bottom of the ultrafiltration centrifuge tube through a duct, and two ends of a third suction apparatus are respectively connected to the ultrafiltration centrifuge tube's part accommodating supernatant and an alpha 2 macroglobulin-rich serum collector through a duct. According to the invention, theseparation process does not destroy the activity of biological molecules, the recovery rate is high and the reproducibility is good.

Description

technical field [0001] The invention relates to the technical field of preparation of blood products, in particular to a device and method for preparing serum rich in α2 macroglobulin by an improved ultrafiltration centrifugation method. Background technique [0002] Post-traumatic Osteoarthritis (PTOA), as a major type of osteoarthritis (OA), is mainly caused by trauma, with articular cartilage degeneration and secondary cartilage ossification as the main pathological changes, and joint pain , activity dysfunction as the main clinical manifestation of a disease. Anterior cruciate ligament (ACL) injury is one of the most common knee ligament injuries in young adults, with an incidence of approximately 0.1% in the United States. Domestic scholars have reported that the overall incidence of ACL injuries in active training athletes in my country is 0.47%. A large number of studies have shown that the risk of PTOA in patients with ACL injury is significantly increased. [000...

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Application Information

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IPC IPC(8): C07K14/81C07K1/34
CPCC07K14/81
Inventor 王小虎魏垒卫小春李鹏翠
Owner 王小虎
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