Digestion-promoting compound microbial agent for biological feed and its application

A technology of biological feed and compound bacterial agent, which is applied in animal feed, animal feed, biochemical equipment and methods, etc., can solve the problems of decreased digestion and absorption efficiency, poor prospects and high processing costs

Active Publication Date: 2020-10-20
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above treatment methods still have the following problems: (1) the crude fiber is not fully hydrolyzed, and the problem of the decline in digestion and absorption efficiency has not been fundamentally solved; (2) the treatment cost is high, the economy is poor, and the prospect of practical application in the industry is still poor
For example, researchers used Clostridium thermocellum to achieve efficient hydrolysis of microcrystalline cellulose in their previous work (Zhang, J., S. Liu, R. Li, W. Hong, Y. Xiao, Y. Feng, Q. Cui and Y.-J.Liu(2017). "Efficient whole-cell-catalyzing celluloseaccharification using engineered Clostridiumthermocellum."Biotechnol Biofuels 10(1):124.), but it has not been applied to the hydrolysis of lignocellulosic substrates As well as the development and preparation of lignocellulose-based feed, and the preparation of chicken feed by using cellulosome-producing microorganisms as feed additives, whether it can promote digestion, increase egg production rate, and reduce feed-to-meat ratio still needs to be explored

Method used

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  • Digestion-promoting compound microbial agent for biological feed and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the preparation of promoting digestion biological feed

[0021] (1) Preparation of cellulosome microbial bacterial agent:

[0022] According to the solid-liquid weight volume ratio of 1:20, the corn stalks and glucose medium after alkaline pretreatment were added to the reaction device, and the inoculum of Clostridium thermocellum was inoculated according to the inoculation amount of 5% volume fraction, and then the Mix evenly at a speed of 1 min; check the pressure in the container regularly and deflate, and end the cultivation when there is no positive pressure in the container; centrifuge for solid-liquid separation, and the obtained solid phase is the cellulosome-producing microbial bacterial agent.

[0023] Described glucose culture medium is that dipotassium hydrogen phosphate 2.9g / L, potassium dihydrogen phosphate 1.5g / L, urea 0.8g / L, calcium chloride 0.1g / L, magnesium chloride 1.8g / L, sulfuric acid are added in every liter of water. Ferrous iron 0...

Embodiment 2

[0028] Embodiment 2: the preparation of promoting digestion biological feed

[0029] Different from Example 1,

[0030](1) Preparation of cellulosome-producing microbial bacterial agents: according to the solid-liquid weight-to-volume ratio of 1:20, add corn cob and glucose medium pretreated by sulfonation method to the reaction device, and inoculate according to 5% volume fraction Xanthocellulolyticus was inoculated in a large amount, and then mixed evenly at 60°C and 100r / min; regularly check the pressure in the container and release the air, and stop the cultivation when there is no positive pressure in the container; centrifuge for solid-liquid separation, and the obtained The solid phase is the cellulosome-producing microbial inoculum.

[0031] (2) Preparation of composite inoculum: according to dry weight, weigh 50 parts by weight of cellulosome microbial inoculum obtained in step (1), 37 parts by weight of lactobacillus inoculum, and 13 parts by weight of Bacillus subt...

Embodiment 3

[0033] Embodiment 3: the preparation of promoting digestion biological feed

[0034] Different from Example 1,

[0035] (1) Preparation of cellulosome-producing microbial bacterial agents: according to the solid-liquid weight-to-volume ratio of 1:30, add corn bran and glucose medium after hydrothermal pretreatment to the reaction device, and inoculate according to 10% volume fraction Inoculate a large amount of Pseudomonas cellulolyticus, and then mix evenly at 55°C and 150r / min; regularly check the pressure in the container and release the air, and stop the cultivation when there is no positive pressure in the container; centrifuge for solid-liquid separation, and the obtained The solid phase is the cellulosome-producing microbial inoculum.

[0036] (2) Preparation of composite inoculum: according to dry weight, weigh 60 parts by weight of cellulosome microbial inoculum obtained in step (1), 20 parts by weight of lactobacillus inoculum, and 20 parts by weight of Bacillus sub...

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Abstract

The invention provides a digestion-promoting compound bacterial agent for biological feed. The digestion-promoting compound bacterial agent is composed of the following components in terms of dry weight and parts by weight: 40-80 parts by weight of the microbial microbial agent, and 15-37 weight of the lactobacillus agent. Parts, 5 to 25 parts of Bacillus subtilis bacteria are mixed and uniformlymixed to obtain a composite microbial agent. The fiber-producing microorganism is Clostridium thermocellum, Clostridium solani or Pseudomonas solani. The present invention also provides a method of preparing a lignocellulosic-based biological feed using the composite microbial agent. The preparation of the lignocellulosic-based biological feed by using the composite microbial agent not only promotes digestion and absorption of food, but also significantly increases the egg production rate, reduces the ratio of feed to meat, and inhibits the growth and reproduction of pathogenic bacteria and reduces the incidence rate. In addition, the incidence of chicken (lowering), egg production rate (increased), broiler feed ratio (improvement) and other effects are significantly better than the basicchicken feed.

Description

technical field [0001] The invention belongs to the technical field of biological feed, and relates to the technology of preparing chicken feed by using lignocellulose, in particular to a digestion-promoting composite bacterial agent for biological feed and the preparation thereof by using the composite bacterial agent to promote digestion, increase egg production rate, and reduce feed consumption. Feed method of meat ratio. Background technique [0002] The key issue in the development of the breeding industry is the health level and production capacity of animals. As one of the most common poultry, chickens have become a steady source of meat and egg food for humans. Therefore, how to accelerate the growth of chicken, improve egg production capacity, and reduce the incidence of disease is a long-term focus in the field of poultry breeding. [0003] Lignocellulosic biomass is the most abundant renewable biomass resource on earth. my country is a large agricultural countr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K10/30A23K10/37A23K10/22A23K10/26A23K20/22C12R1/125C12R1/225C12R1/145C12R1/01
CPCA23K10/18A23K10/22A23K10/26A23K10/30A23K10/37A23K20/22C12N1/20Y02P60/87
Inventor 崔球初欢欢刘亚君刘世岳李仁民
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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