Digestion-promoting compound microbial agent for biological feed and its application
A technology of biological feed and compound bacterial agent, which is applied in animal feed, animal feed, biochemical equipment and methods, etc., can solve the problems of decreased digestion and absorption efficiency, poor prospects and high processing costs
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Embodiment 1
[0020] Embodiment 1: the preparation of promoting digestion biological feed
[0021] (1) Preparation of cellulosome microbial bacterial agent:
[0022] According to the solid-liquid weight volume ratio of 1:20, the corn stalks and glucose medium after alkaline pretreatment were added to the reaction device, and the inoculum of Clostridium thermocellum was inoculated according to the inoculation amount of 5% volume fraction, and then the Mix evenly at a speed of 1 min; check the pressure in the container regularly and deflate, and end the cultivation when there is no positive pressure in the container; centrifuge for solid-liquid separation, and the obtained solid phase is the cellulosome-producing microbial bacterial agent.
[0023] Described glucose culture medium is that dipotassium hydrogen phosphate 2.9g / L, potassium dihydrogen phosphate 1.5g / L, urea 0.8g / L, calcium chloride 0.1g / L, magnesium chloride 1.8g / L, sulfuric acid are added in every liter of water. Ferrous iron 0...
Embodiment 2
[0028] Embodiment 2: the preparation of promoting digestion biological feed
[0029] Different from Example 1,
[0030](1) Preparation of cellulosome-producing microbial bacterial agents: according to the solid-liquid weight-to-volume ratio of 1:20, add corn cob and glucose medium pretreated by sulfonation method to the reaction device, and inoculate according to 5% volume fraction Xanthocellulolyticus was inoculated in a large amount, and then mixed evenly at 60°C and 100r / min; regularly check the pressure in the container and release the air, and stop the cultivation when there is no positive pressure in the container; centrifuge for solid-liquid separation, and the obtained The solid phase is the cellulosome-producing microbial inoculum.
[0031] (2) Preparation of composite inoculum: according to dry weight, weigh 50 parts by weight of cellulosome microbial inoculum obtained in step (1), 37 parts by weight of lactobacillus inoculum, and 13 parts by weight of Bacillus subt...
Embodiment 3
[0033] Embodiment 3: the preparation of promoting digestion biological feed
[0034] Different from Example 1,
[0035] (1) Preparation of cellulosome-producing microbial bacterial agents: according to the solid-liquid weight-to-volume ratio of 1:30, add corn bran and glucose medium after hydrothermal pretreatment to the reaction device, and inoculate according to 10% volume fraction Inoculate a large amount of Pseudomonas cellulolyticus, and then mix evenly at 55°C and 150r / min; regularly check the pressure in the container and release the air, and stop the cultivation when there is no positive pressure in the container; centrifuge for solid-liquid separation, and the obtained The solid phase is the cellulosome-producing microbial inoculum.
[0036] (2) Preparation of composite inoculum: according to dry weight, weigh 60 parts by weight of cellulosome microbial inoculum obtained in step (1), 20 parts by weight of lactobacillus inoculum, and 20 parts by weight of Bacillus sub...
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