Bacteriophage composition and application in inactivating antibiotic-resistant pathogenic bacteria

Abstract

A bacteriophage composition and its application in inactivating antibiotic-resistant pathogenic bacteria, comprising three bacteriophages deposited in China Typical Culture Collection on Aug. 1, 2018,respectively phi. YSZKA, with the accession number of CCTCC M 2018513; Phage [phi] YSZKP, deposited as CCTCC M 2018514; phage [phi] YSZPA, preservation number is CCTCC M 2018515. A method and apparatus for treat contaminated soil Adding specific bacteriophage solution to plant system, directionally infecting and inactivating the system of resistant pathogenic bacteria combined pollution to remove the resistance gene. After the restoration, the diversity and stability of soil ecological environment function was significantly restored.

Description

technical field [0001] The invention belongs to the technical field of remediation of multiple antibiotic-resistant pathogenic bacteria compound polluted soil, and in particular relates to a method for multivalent phage therapy targeting inactivation of antibiotic-resistant pathogenic bacteria in a soil-vegetable system. Background technique [0002] Bacteriophages (phages for short) are a type of organisms that exclusively prey on living host bacteria and are widely distributed in soil, water, air, and even on the surface or intestines of humans and animals. It is estimated that the total amount reaches 10 31 Order of magnitude; Polyvalent Phage Therapy (Polyvalent Phage Therapy) refers to artificially accelerating the expression of its broad host spectrum after isolating, screening, purifying and enriching the exclusive phage of the host bacterium, and screening for high titer, short lysis period, and stress resistance Strong multivalent phage, and then add specific phag...

AI Technical Summary

Problems solved by technology

[0004] Through relevant literature review and patent search, no public publication and acceptance of multivalent phage therapy on complex high-abundance antibiotic-resistant pathogenic bacteria and resistant gene-contaminated soil-vegetable system bioremediation technology has been found, which is the closest to the present invention The existing method is phage therapy for mammalian pathogenic bacterial infection and the application of phage therapy related to bacterial wilt in crops. Highly specific phages attack and inactivate Pseudomonas aeruginosa, Escherichia coli, Xanthomonas and Ralstonia solanacearum. The current phage therapy is mainly to specifically attack the inactivation process of a host bacterium, however The remediation technology for combined pollution of resistant pathogenic bacteria in the soil-vegetable system is very rare, and the application number: CN201580008049.3 / CN201580008049.4 respectively provides a phage combination agent against Pseudomonas aeruginosa / Escherichia coli infection The treatment method, the invention uses specific pathogenic bacteria as host bacteria to screen a large number of phages in environmental samples, and selects a combination of highly active phages as raw materials for pharmaceutical preparation, which can perform "multiple pairs" against Pseudomonas aeruginosa / Escherichia coli One" high-efficiency inactivation; application number: CN201610924016.0 provides an application technology of Salmonella bacteriophage and its mixture in food systems, the method is mainly to screen the exclusive Potent phage, using the mixture of these two phages to prevent and control the spread of Salmonella bacteria in the field of food safety; application number: CN201610014708.1 provides a biological control technology for rice leaf blight by phage therapy, the method It is mainly to screen out a virulent phage that inactivates Xanthomonas rice leaf blight with high specificity from the soil environment, aiming at the prevention and treatment of rice leaf blight; application number: CN201510008569.7 provides a phage Therapy is a technical method for the prevention and treatment of tobacco bacterial wilt. The method is mainly to inject the prepared phage suspension into the tobacco stem with a sterile syringe, and cover it with mineral oil on the outside

These four methods apply phages to the diseased site to specifically attack and inactivate pathogenic bacteria, and lack the overall repair effect on the soil-crop (vegetable) system to remove pathogenic bacteria synergistically

In addition, the existing patents have never involved the use of phage therapy to inactivate pathogenic bacteria carrying antibiotic resistance genes in soil-vegetable systems

[0005] The main disadvantages of the existing technology are: most of the existing phage therapy applications select specific and highly specific phages as raw materials, and one strain of phage corresponds to "predating" a host bacterium, the treatment system is too single, and the preparation of medicaments is cumbersome and complicated. Biocontrol technology for synchronous inactivation of multiple antibiotic-resistant pathogenic bacteria co-contamination in soil-vegetable systems, while the associated ecological risk assessment for the functional stability and diversity of microbial communities in soil-vegetable systems after application has received little attention

[0006] The main reasons for the defects are as follows: In recent years, the academic community has gradually recognized that the soil-vegetable system is the "source" and "sink" for the accumulation and occurrence of various antibiotic-resistant bacteria and resistance genes, and this type of new resistance causes Pathogenic bacteria and genes will seriously threaten human health and ecological environment safety through the transmission of food chains; however, most of the existing research is that one or more phages specifically attack a certain "species" of host bacteria, and have a high-abundance in the soil-vegetable system. Contamination of compound pathogenic bacteria is less concerned, therefore, it is urgent to carry out research and development of biological targeted inactivation technology to reduce and eliminate the risk of accumulation of antibiotic-resistant pathogenic bacteria in soil-vegetable systems

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