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A kind of methionine oligopeptide and its preparation method and application

A technology of methionine and methionine ethyl ester hydrochloride, applied in the field of methionine oligopeptide, can solve the problems of many reagents, low yield, complicated reaction and the like, and achieve the effects of easy operation, high yield and simple preparation process

Active Publication Date: 2020-12-01
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the methionine oligopeptide prepared by this preparation method is a mixture of dipeptide to octapeptide, and the yield is low, and the reaction process uses more reagents, and the reaction is more loaded down with trivial details

Method used

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  • A kind of methionine oligopeptide and its preparation method and application
  • A kind of methionine oligopeptide and its preparation method and application
  • A kind of methionine oligopeptide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] This embodiment is a preparation method of methionine oligopeptide, which preparation method comprises the following steps:

[0062] (a) dissolving papain in 1.2M potassium phosphate buffer solution with pH7.2, centrifuging at 10000r / min for 6min at 23°C to obtain a papain solution with a concentration of 28mg / ml;

[0063] (b) Dissolve L-Met-OEt.HCl in 1.2M potassium phosphate buffer at pH 7.2, and bathe in water at 45°C for 0.5-1.5min to obtain L-Met-OEt.HCl with a concentration of 0.24-0.26M solution;

[0064] (c) Mix the protease solution and the L-Met-OEt.HCl solution at a volume ratio of 1:1.2, and react at 52°C for 10 hours, and then heat the reaction solution to 90°C for 6 minutes;

[0065] (d) cooling the inactivated mixed solution, centrifuging to collect the precipitate, washing the precipitate with water, and freeze-drying to obtain the methionine oligopeptide.

[0066] Weigh the methionine oligopeptide prepared by the above method, and calculate the yield ...

Embodiment 2

[0068] This embodiment is a preparation method of methionine oligopeptide, which preparation method comprises the following steps:

[0069] (a) dissolving papain in 0.8M potassium phosphate buffer solution with pH7.0, centrifuging at 15000r / min for 4min at 27°C to obtain a papain solution with a concentration of 22mg / ml;

[0070] (b) Dissolve L-Met-OEt.HCl in 0.8M potassium phosphate buffer solution with pH 7.0, and bathe in water at 55°C for 0.5-1.5min to obtain a 0.26M L-Met-OEt.HCl solution;

[0071] (c) Mix the protease solution and the L-Met-OEt.HCl solution at a volume ratio of 1:0.8, and react at 48°C for 10 hours, and then heat the reaction solution to 92°C for 4 minutes;

[0072] (d) cooling the inactivated mixed solution, centrifuging to collect the precipitate, washing the precipitate with water, and freeze-drying to obtain the methionine oligopeptide.

[0073] According to the yield calculation method in Example 1, the yield of methionine oligopeptide prepared by ...

Embodiment 3

[0075] This embodiment is a preparation method of methionine oligopeptide, which preparation method comprises the following steps:

[0076] (a) dissolving papain in 1M potassium phosphate buffer solution with pH 7.0, centrifuging at 12000r / min for 5min at 25°C to obtain a papain solution with a concentration of 25mg / ml;

[0077] (b) Dissolve L-Met-OEt.HCl in 1M potassium phosphate buffer solution with pH 7.0, and bathe in water at 50°C for 1 min to obtain a 0.25M L-Met-OEt.HCl solution;

[0078] (c) Mix the protease solution and the L-Met-OEt.HCl solution at a volume ratio of 1:1, and react at 50°C for 12 hours, then heat the reaction solution to 90°C for 5 minutes;

[0079] (d) cooling the inactivated mixed solution, centrifuging to collect the precipitate, washing the precipitate with water, and freeze-drying to obtain the methionine oligopeptide.

[0080] According to the yield calculation method in Example 1, the yield of methionine oligopeptide prepared by the above meth...

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Abstract

The invention relates to a methionine oligopeptide, a preparation method and an application thereof, wherein the preparation method of the methionine oligopeptide comprises the following steps: the protease is dissolved in a buffer solution and centrifuged to obtain a concentration of 22-28mg / ml protease solution; L-Met-OEt. HCl was dissolved in buffer and bathed in water to give a concentration of 0.24-0.26 M L-Met-OEt. HCl solution; Subject that protease solution to L-Met- The OEt. HCl solution was mixed at a volume ratio of 1: (0.8-1.2), and mixed at 48-52 DEG C, and then inactivate that protease in the reaction solution; Cooling the inactivated mixture, centrifugally collecting the precipitate, washing the precipitate with water, and freeze-drying to obtain methionine oligopeptide; A methionine oligopeptide contain any one or more components of that methionine dipeptide to the decapeptide can be obtain by the preparation method, and the yield is high; In addition, less raw materials are used, the preparation process is simple and easy to operate.

Description

technical field [0001] The invention relates to the technical field of methionine oligopeptides, in particular to a methionine oligopeptide and its preparation method and application. Background technique [0002] Methionine is an important limiting essential amino acid in plant protein sources. It is widely used in livestock and poultry aquatic feed. In order to achieve the purpose of amino acid balance, it is often supplemented in the form of crystal amino acid salt or embedding; Crystalline methionine is easily dissolved and lost in water, and the absorption of crystalline methionine and protein-bound methionine is not synchronized; the utilization rate of methionine is reduced. [0003] For example, the Chinese patent application with the publication number CN102429109A discloses a methionine oligopeptide. The preparation method of the methionine oligopeptide is as follows: first, methionine ethyl ester hydrochloride (H-Met-OEt.HCl) is formulated into 300mg / ml The pH is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/16A23K20/147
CPCA23K20/147C07K1/16C12P21/06
Inventor 张文兵谢方丽徐玮麦康森
Owner OCEAN UNIV OF CHINA
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