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Analysis apparatus and focusing method

An analysis device and a technology for the direction of the optical axis, applied in the direction of analysis materials, image analysis, measurement devices, etc., can solve the problems of low focus accuracy, low contrast, discomfort, etc., and achieve the effect of high focus accuracy

Active Publication Date: 2019-01-18
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the contrast method, there is a problem that the focus accuracy decreases when the contrast is low and the object is relatively small relative to the imaging range.
Sediment components in urine are small objects with low contrast with urine, so they are not suitable subjects for the contrast method

Method used

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  • Analysis apparatus and focusing method

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no. 1 approach

[0028] figure 1 It is a diagram showing a schematic configuration of the imaging device 1 of the first embodiment. The imaging device 1 images, for example, urine as a sample, and is used as an analysis device for analyzing, for example, formed components in urine by analyzing the imaged images. However, the imaging device 1 can also be applied to the analysis of formed components in liquid samples other than urine, such as cells and body fluids.

[0029] The imaging device 1 has a camera 11 for imaging a sample, a light source 12 for imaging, and a flow cell unit 13 . The flow cell unit 13 has a stage 13B on which a flow cell 13A through which a sample flows is fixedly arranged. The flow cell 13A is also detachable with respect to the stage 13B. Additionally, the figure 1 In the illustrated imaging device 1 , the longitudinal direction is defined as the X-axis direction in the rectangular coordinate system, the width direction is defined as the Y-axis direction, and the h...

no. 2 approach

[0089]In the second embodiment, focus point determination processing is different from that in the first embodiment. Other devices, controls, and the like are the same as those of the first embodiment, and thus description thereof will be omitted. Here, regarding the focal point determination processing described in the first embodiment, the processing is simple and the calculation speed is increased, but the robustness against images with a low signal-to-noise ratio is low. Therefore, in the second embodiment, the robustness is improved by comparing the inside and outside of the mask image.

[0090] Figure 11 It is a diagram for explaining focus point determination processing. The creation of the mask image 32 , the creation of the blurred image 33 , and the creation of the difference image 34 are the same as those in the first embodiment. In addition, the following will Figure 11 The range corresponding to the covered range (blackened range) in the mask image 32 is cal...

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Abstract

The invention provides an analysis apparatus and a focusing method. Images of tangible components in a detection body with high focusing precision are acquired. An analysis apparatus comprises a flowcell which has a flow passage for a liquid containing a tangible component; an image pickupper which picks up images of the liquid flowing through the flow passage; an adjuster which adjusts a relative position of the flow cell with respect to the image pickupper in relation to an optical axis direction of the image pickupper and a direction in which the liquid flows through the flow passage; anda controller which determines an image pickup position of the flow cell in at least one of the optical axis direction and the direction in which the liquid flows, on the basis of a number of pieces ofthe tangible component in focusing states existing in the images of the liquid picked up by the image pickupper at a plurality of positions at which the relative position differs.

Description

technical field [0001] The invention relates to an analysis device and a method of focusing. Background technique [0002] In the examination of collected urine, there is known a method in which the urine sample is photographed while the specimen is flowing in a flow cell, and the sediment components (blood cells, epithelial cells) in the urine are analyzed by analyzing the photographed image. , columns, bacteria, crystals, and other tangible (solid) components in urine) (for example, refer to Patent Document 1). [0003] Patent Document 1: Japanese Patent Application Laid-Open No. 10-104229 [0004] When photographing the formed components in the sample flowing in the flow cell, it is considered to perform autofocus control by the contrast method, which is one of the focusing methods. However, in the contrast method, there is a problem that focus accuracy decreases when the contrast is low and the object is relatively small relative to the imaging range. The sediment com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H04N5/232
CPCH04N23/67G01N2015/1452G01N2015/1413G01N15/1404G01N15/147G01N15/1484G01N15/1434G01N33/493H04N23/675H04N23/683G06T7/0016G06T2207/30024
Inventor 增田茂树中西健二
Owner ARKRAY INC
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