Compound with immunity-enhancing effect and application thereof in preparation of medicine for improving immunity

A compound and immunity technology, applied in the field of preparation of immunity-enhancing drugs, can solve the problems of poor water solubility, unstable chemical sites, and far-reaching efficacy of andrographolide, and achieve the purpose of improving immunity. Active application, extraction The separation method is simple and has no toxic and side effects

Inactive Publication Date: 2019-01-22
ZHUZHOU QIANJIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, further studies have found that andrographolide has poor water solubility, multiple unstable chemical sites, less absorption and low bioavailability in the body, which greatly limits its application.
Moreover, the composition of diterpene lactones in Andrographis paniculata is complex, about 40 kinds have been found so far, and the subtle differences in the structure of different compounds will also make their efficacy vary greatly
[0006] At present, the active mechanism of diterpene lactones in Andrographis paniculata has not yet had definite research results. At the same time, there is an urgent need in this field for new, high-efficiency, plant-derived compounds that improve immunity

Method used

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  • Compound with immunity-enhancing effect and application thereof in preparation of medicine for improving immunity
  • Compound with immunity-enhancing effect and application thereof in preparation of medicine for improving immunity
  • Compound with immunity-enhancing effect and application thereof in preparation of medicine for improving immunity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] S1. According to the method under the Fuke Qianjin Capsule in the Pharmacopoeia of the People's Republic of China [1] Extract from Zhuzhou Qianjin Pharmaceutical Co., Ltd. and prepare 1.5kg of Fuke Qianjin Prescription Extract Dry Cream (FKQJ). This dry paste was extracted 8 times with 3 times the volume of EtOAc to obtain an EtOAc extract (FKQJE, 156g);

[0033] S2. Take the EtOAc extract (FKQJE, 142.2g) and mix the sample with silica gel, then go through silica gel (1kg, 200-300 mesh) column chromatography, cyclohexane-EtOAc (9:1, 8:2, 7:3, 6:4 , 5:5, v / v) gradient elution, TLC detection and merging of similar fractions, a total of 10 fractions were obtained, respectively named: Fr.1, Fr.2, Fr.3, Fr.4, Fr. 5. Fr.6, Fr.7, Fr.8, Fr.9, Fr.10, spare;

[0034] S3. the fraction Fr.5 (11g) collected in step S2 is removed pigment (MeOH:H by MCI column chromatography) 2 O=5:5, 6:4, 7:3, 8:2, 9:1, v / v), TLC detection and merging of similar fractions, a total of 3 sub-fractio...

Embodiment 2

[0043] Embodiment 2 The effect of the compound of the present invention on mouse spleen lymphocyte proliferation

[0044] 1. Experimental method

[0045] (1) Spleen lymphocyte culture

[0046] Balb / c pure line mice, male or female, 8 weeks old. The mice were killed by cervical dislocation, the spleens of the mice were aseptically removed, cut into pieces and ground in RPMI1640 complete medium, and filtered through a 180-mesh sieve to obtain a single-cell suspension, and the number of cells was adjusted to 1×10 6 / mL. Add the cell suspension into a sterilized 96-well cell culture plate, 100 μl per well;

[0047] (2) Experimental grouping

[0048] The experiment was divided into blank group, lipopolysaccharide group and concanavalin A group, all of which were added with RPMI1640 complete medium containing different components, with a volume of 100 μl.

[0049] Blank group: add 100 μl RPMI1640 complete medium;

[0050] Lipopolysaccharide (LPS) group: add LPS to a final conc...

Embodiment 3

[0061] Embodiment 3 The effect of the compounds of the present invention on the phagocytosis of macrophages

[0062] 1. Experimental method

[0063] (1) Experimental grouping

[0064] 70 Balb / c pure-line mice, weighing 18-22 g, half male and half male, were randomly divided into 7 groups, 10 mice per group. The experiment set up a blank control group, a cyclophosphamide group (CY group), and 5 drug administration groups.

[0065] The blank control group was not injected;

[0066] CY group: inject cyclophosphamide 50mg / kg twice;

[0067] 5 administration groups: intragastric administration of drugs Fr.5-110mg / kg, 50mg / kg, 100mg / kg, 200mg / kg, 500mg / kg every day.

[0068] (2) Experimental treatment

[0069] The blank control group and CY group were intragastrically administered distilled water for 2 consecutive weeks (14 days), once a day; the treatment group was intragastrically administered for 2 consecutive weeks (14 days), once a day; The drug group was intraperitoneall...

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PUM

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Abstract

The invention discloses a compound with immunity-enhancing effect and application thereof in preparation of an antibacterial medicine. The structural formula of the compound is as shown in a formula (I) in the description. The compound has a good immunity-enhancing effect. The compound is obtained through extraction, isolation and purification from a traditional Chinese medicine Fuke Qianjin tablet or Fuke Qianjin capsule by means of modern technology. Experiments prove that the compound shows obvious effects of improving immunity, can significantly promote proliferation of lymphocytes, increase the phagocytic function of macrophages, and / or increase the number of white blood cells.

Description

technical field [0001] The present invention relates to the field of efficacy of compounds, more specifically, relates to a compound capable of improving immunity and its application in the preparation of medicines for improving immunity. Background technique [0002] Immunity is the body's own defense mechanism. It is the body's ability to recognize and eliminate any foreign bodies (viruses, bacteria, etc.) Ability is the physiological response of the human body to recognize and eliminate "dissidents". [0003] Immunity can be divided into non-specific immunity and specific immunity: 1. Innate immunity, which is present at birth; 2. Acquired immunity, which is acquired naturally in the course of life after birth, or artificially assisted. The method is passively obtained. [0004] In modern society, environmental problems are becoming more and more prominent. People are under high work pressure and lack of exercise, which leads to the deterioration of human immunity. Impr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/365A61P37/04A23L33/105
CPCA23V2002/00A61K31/365A23L33/105A61P37/04A23V2200/324A23V2250/21
Inventor 张鹏袁红波王康华杨秀伟龚云凌勇根白璐郑元青赵毅
Owner ZHUZHOU QIANJIN PHARMA
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