A kit and method for detecting swine-derived ingredients

A kit and porcine-derived technology, applied in the field of molecular biology, can solve the problems of poor primer binding efficiency, false positives, and poor amplification curves, and achieve the effects of broad application prospects, high sensitivity and rapid detection.

Pending Publication Date: 2019-01-25
成都市食品药品检验研究院 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Part 8 of the existing standard "SN/T 3730-2013 Identification Methods of Common Animal Breeds in Food and Feed" uses real-time fluorescent PCR method to detect pig-derived components in food and feed, but we found during the detection process that This method may cause false positives during detection. For example, according to the Ct value of goose components, it may be judged as pig-derived components, and the accuracy of the determination results cannot be guaranteed.
[0005] The PCR method for the detection of pig-derived components reported by Wang Ying (Wang Ying, etc., fluorescent quantitative PCR method for detecting pig-derived components in livestock meat [J], Journal of Food Safety and Quality Inspection, 2013, 5:1...

Method used

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  • A kit and method for detecting swine-derived ingredients
  • A kit and method for detecting swine-derived ingredients
  • A kit and method for detecting swine-derived ingredients

Examples

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Embodiment 1

[0050] Example 1 The present invention detects the kit and detection method of pig-derived components

[0051] One, kit composition of the present invention

[0052] Including the primer pair shown in SEQ ID NO.1, SEQ ID NO.2 and the probe shown in SEQ ID NO.3; as shown in Table 1:

[0053] Table 1 Primers and probes of the present invention

[0054]

[0055] Note: F is the 5' end primer, R is the 3' end primer, and P is the probe.

[0056] Two, adopt the kit of the present invention to detect pig-derived components

[0057] 1. DNA extraction

[0058] After the sample is homogenized, weigh 0.1g to 0.2g, put it in a 2mL centrifuge tube, add 600μL CTAB lysate and 20μL proteinase K, shake and mix, incubate at 65°C for 1h to 2h, and shake and mix every 10min during the period; add 500 μL of phenol: chloroform: isoamyl alcohol (25:24:1), shake well, centrifuge at 12 000 g for 5 min; carefully pipette the supernatant into a clean 1.5 mL centrifuge tube, add an equal volume of...

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Abstract

A kit for detect a swine-derived component comprising a prim pair shown in SEQ ID NO. 1 to SEQ ID NO. 2 and a probe shown in SEQ ID NO. 3, and a method for detecting a swine-derived component. The kitand the method of the invention can accurately detect pig-derived ingredients in meat and meat products, and the kit and the method have the advantages of fast detection, accuracy, low cost, good specificity, high sensitivity, and wide application prospect.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a kit and a method for detecting components derived from pigs. Background technique [0002] In recent years, with the rapid expansion of the meat market, incidents of meat adulteration have occurred frequently. There are more and more ways of adulteration, and the forms are becoming more and more complicated. It has become a prominent problem in the global food industry. The adulteration of meat products will not only cause individual health risks, including disease infection, metabolic disorders, allergic reactions, etc., but also more likely to cause economic, religious, and moral problems. For example, halal food mixed with pig-derived ingredients that are not listed in the ingredient list will seriously violate the religious beliefs and ethnic habits of ethnic minorities in my country and Islamic countries such as Arab countries, and infringe consumers' right to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888
Inventor 张玉董娟梁恒兴杜凤段庆梓陈浩东尚柯王巍吴文林黄春燕张彪
Owner 成都市食品药品检验研究院
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