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Preparation method of animal antiserum

An antiserum, animal technology, applied in the direction of serum immunoglobulin, etc., can solve the problems of low quality, complicated operation, low yield, etc., and achieve the effect of low cost and simple process

Inactive Publication Date: 2019-02-12
天津市正江现代生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the method for preparing antiserum has the characteristics of complicated operation, low yield and low quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] Below in conjunction with embodiment the present invention will be further described.

[0019] A method for extracting animal antiserum, comprising the following steps:

[0020] (1) Choose a 3-year-old, healthy, non-infected red deer, and immunize the animals. During the immunization process, the nutritional status of the animals and the sanitary conditions of the animals must be maintained in good condition;

[0021] (2) After 10 days of the last immunization, collect blood samples from the animals to detect their antibody titers. After the antibody titers meet the requirements, select the arterial blood of the animals in the morning when the animals are fasting with sterile blood collection instruments, and add the original Blood volume 10% anticoagulant is 4% sodium citrate solution, stir to mix the anticoagulant evenly;

[0022] (3) Put the blood in a sterilized centrifuge tube and centrifuge at 2000rpm at 4°C for 10 minutes, take the supernatant into a sterilized ...

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Abstract

The invention provides a preparation method of animal antiserum. The method comprises the steps of selecting an immune animal, detecting antibody titers in an immunologic process, collecting blood, extracting antiserum by means of two-time centrifugal separation, purifying the serum by means of membrane filtration, and carrying out inactivation treatment on viruses. Compared with the prior art, the extraction method, provided by the invention of the animal antiserum is low in cost and simple in process, does not introduce other impurities, and can be used for stably extracting the high-qualityantiserum.

Description

technical field [0001] The invention relates to the field of therapeutic biological products, in particular to a method for preparing animal antiserum. Background technique [0002] With the development of science and technology, new technologies and products in the field of veterinary biological products continue to emerge and have been widely used. Animal antiserum belongs to the field of therapeutic biological products. Antiserum is also called hyperimmune serum or anti-disease serum. It is an animal serum containing high titers of specific antibodies. In the prior art, the method for preparing antiserum has the characteristics of complicated operation, low yield and low quality. Contents of the invention [0003] In order to solve the technical problems in the prior art, the invention provides a preparation method of animal antiserum. [0004] To achieve the above object, the present invention is achieved through the following technical solutions: [0005] A method ...

Claims

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Application Information

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IPC IPC(8): C07K16/06
CPCC07K16/06
Inventor 陈燕
Owner 天津市正江现代生物技术有限公司