A method for collecting microorganisms on the surface of aged tobacco leaves
A technology for microorganisms and tobacco leaves, applied in the field of applied microbiology, can solve the problems of low collection efficiency of microorganisms on the surface of aged tobacco leaves, incomplete microorganisms on the surface of aged tobacco leaves, and inability to analyze the 16sRNA of microorganisms on the surface of aged tobacco leaves, etc. Low cost, good emulsification effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] The method for collecting microorganisms on the surface of aged tobacco leaves of the present embodiment comprises the following steps:
[0026] Step (1), equipped with 10% SDS solution for standby
[0027] Weigh 10g of SDS solid and dissolve it in 100mL of distilled water to make a 10% SDS solution, and place it at room temperature after sterilization.
[0028] Step (2), extraction
[0029] Weigh 10g of aged tobacco leaves, cut into an area of 1cm 2 The fragments were transferred to an Erlenmeyer flask filled with 100mL of sterile 0.85% sodium chloride solution, added 1‰ volume (ie 100μL) of 10% SDS solution, and placed in a shaker at 15°C and 150rpm / min Shake for 30 minutes, then filter with gauze to remove debris such as aged tobacco leaves, then centrifuge at 11,000 g for 20 minutes, discard the supernatant to retain the precipitate.
[0030] Step (3), ultrasonic treatment
[0031] Add 2.5ml of 0.85% sodium chloride solution to the above precipitate, and ultra...
Embodiment 2
[0036] The method for collecting microorganisms on the surface of aged tobacco leaves of the present embodiment comprises the following steps:
[0037] Step (1), equipped with 10% SDS solution for standby
[0038] Weigh 10g of SDS solid and dissolve it in 100mL of distilled water to make a 10% SDS solution, and place it at room temperature after sterilization.
[0039] Step (2), extraction
[0040] Weigh 10g of aged tobacco leaves, cut into an area of 1cm 2 The fragments were transferred to an Erlenmeyer flask containing 100 mL of sterile 0.85% sodium chloride solution, 2‰ volume (200 μL) of 10% SDS solution was added, and placed in a shaker at 16°C and 160 rpm / min Shake for 25 minutes, then filter with gauze to remove debris such as aged tobacco leaves, and then centrifuge at 11000g for 18 minutes, discard the supernatant to retain the precipitate.
[0041] Step (3), ultrasonic treatment
[0042] Add 3ml of 0.85% sodium chloride solution to the above precipitate, and ul...
Embodiment 3
[0047] The method for collecting microorganisms on the surface of aged tobacco leaves of the present embodiment comprises the following steps:
[0048] Step (1), equipped with 10% SDS solution for standby
[0049] Weigh 10g of SDS solid and dissolve it in 100mL of distilled water to make a 10% SDS solution, and place it at room temperature after sterilization.
[0050] Step (2), extraction
[0051] Weigh 10g of aged tobacco leaves, cut into an area of 1cm 2 The fragments were transferred to an Erlenmeyer flask filled with 100mL of sterile 0.85% sodium chloride solution, added 3‰ volume (ie 300μL) of 10% SDS solution, and placed in a shaker at 16°C and 160rpm / min Shake for 35 minutes, then filter with gauze to remove debris such as aged tobacco leaves, then centrifuge at 11,000g for 22 minutes, discard the supernatant to retain the precipitate.
[0052] Step (3), ultrasonic treatment
[0053]Add 2ml of 0.85% sodium chloride solution to the above precipitate, and ultrasoni...
PUM
| Property | Measurement | Unit |
|---|---|---|
| mass | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More