A kind of probiotic starter and its application in the fermentation preparation of fermented milk with ethanol degradation function
A starter and probiotic technology, applied in the field of probiotic starter and its application in fermentation to prepare ethanol-degradable functional fermented milk, to achieve the effect of high live bacteria content
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[0032] The present invention also provides the preparation method of described probiotic starter, comprising the following steps:
[0033] 1) Inoculate Lactobacillus acidophilus, Streptococcus thermophilus and Lactobacillus bulgaricus into MRS liquid medium after activation, domestication and proliferation, respectively, and carry out seed liquid culture to obtain seed liquid of Lactobacillus acidophilus and Streptococcus thermophilus and the seed liquid of Lactobacillus bulgaricus;
[0034] 2) mixing the seed liquid of the Lactobacillus acidophilus, the seed liquid of the Streptococcus thermophilus and the seed liquid of the Lactobacillus bulgaricus into the MRS liquid medium for fermentation and culture to obtain a mixed fermentation liquid;
[0035] 3) centrifuge the mixed fermentation broth described in step 2), and collect the solid phase components as probiotic starter.
[0036] In the present invention, firstly, the Lactobacillus acidophilus, Streptococcus thermophilus...
Embodiment 1
[0051] Preparation of probiotic starter
[0052] Proceed as follows:
[0053] 1) Domestication and proliferation of probiotics: Inoculate the lyophilized powder of three strains of Lactobacillus acidophilus, Streptococcus thermophilus, and Lactobacillus bulgaricus into 10 mL of sterilized MRS broth medium, vortex and mix them at a constant temperature of 37°C for activation After 24 hours, the activated strains were inoculated into skim milk medium at an inoculum size of 2% by volume for domestication and proliferation, and cultured at a constant temperature of 40°C for 10 hours until curdling;
[0054] 2) Preparation of probiotic starter: the culture fluids of the three domesticated and multiplied strains were respectively inserted into the MRS liquid culture with an inoculum volume ratio of 2% and cultivated at a constant temperature of 37°C for 24 hours to obtain seed fluids. Bacillus: Streptococcus thermophilus: Lactobacillus bulgaricus with a volume ratio of 2:1:1 was mi...
Embodiment 2
[0061] Preparation of probiotic starter
[0062] 1) Domestication and proliferation of probiotics: Inoculate the lyophilized powder of three strains of Lactobacillus acidophilus, Streptococcus thermophilus, and Lactobacillus bulgaricus into 10 mL of sterilized MRS broth medium, vortex and mix them at a constant temperature of 37°C for activation After 24 hours, inoculate the three activated strains into the skim milk medium with an inoculum size of 4% by volume to acclimatize and proliferate, and culture at a constant temperature of 40°C for 8 hours until the milk curds;
[0063] 2) Preparation of probiotic starter: the culture fluids of the three domesticated and multiplied strains were respectively inserted into the MRS liquid culture with an inoculum volume ratio of 2% and cultivated at a constant temperature of 37°C for 24 hours to obtain seed fluids. Bacillus: Streptococcus thermophilus: Lactobacillus bulgaricus with a volume ratio of 2:1:1 was mixed, and then the inoculu...
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